Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli BL21 | Pyrococcus horikoshii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.025 | - |
NADP+ | pH 7.6, 90°C | Pyrococcus horikoshii | |
0.037 | - |
NADPH | pH 7.6, 90°C | Pyrococcus horikoshii | |
0.083 | - |
NADH | pH 7.6, 90°C | Pyrococcus horikoshii | |
0.53 | - |
2-oxoglutarate | pH 7.6, 90°C | Pyrococcus horikoshii | |
0.6 | - |
L-glutamate | pH 7.6, 90°C | Pyrococcus horikoshii | |
2.82 | - |
NH4+ | pH 7.6, 90°C | Pyrococcus horikoshii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48000 | - |
6 * 48000, SDS-PAGE | Pyrococcus horikoshii |
290000 | - |
gel filtration | Pyrococcus horikoshii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus horikoshii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pyrococcus horikoshii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate + NADH + NH3 | no activity with NAD+ as cofactor | Pyrococcus horikoshii | L-glutamate + NAD+ + H2O | - |
ir | |
L-glutamate + NADP+ + H2O | - |
Pyrococcus horikoshii | 2-oxoglutarate + NADPH + NH3 | - |
r |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 48000, SDS-PAGE | Pyrococcus horikoshii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
- |
Pyrococcus horikoshii |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | 100 | 70°C: about 35% of maximal activity, 100°C: about 65% of maximal activity, reductive amination of 2-oxoglutarate | Pyrococcus horikoshii |
80 | 100 | 80°C: about 50% of maximal activity, 100°C: about 60% of maximal activity, oxidative amination of ketoglutarate | Pyrococcus horikoshii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
8 h, enzyme retains more than 95% of its activity | Pyrococcus horikoshii |
90 | - |
8 h, about 35% loss of activity | Pyrococcus horikoshii |
100 | - |
8 h, about 90% loss of activity. 6 h, about 50% loss of activity | Pyrococcus horikoshii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
43.8 | - |
NH4+ | pH 7.6, 90°C | Pyrococcus horikoshii | |
121.2 | - |
L-glutamate | pH 7.6, 90°C | Pyrococcus horikoshii | |
165.3 | - |
2-oxoglutarate | pH 7.6, 90°C | Pyrococcus horikoshii | |
374.5 | - |
NADH | pH 7.6, 90°C | Pyrococcus horikoshii | |
387.5 | - |
NADPH | pH 7.6, 90°C | Pyrococcus horikoshii | |
399.6 | - |
NADP+ | pH 7.6, 90°C | Pyrococcus horikoshii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
- |
Pyrococcus horikoshii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Pyrococcus horikoshii | |
NADP+ | no activity with NAD+ | Pyrococcus horikoshii | |
NADPH | - |
Pyrococcus horikoshii |