Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.4.1.3 extracted from

  • Lee, W.K.; Shin, S.; Cho, S.S.; Park, J.S.
    Purification and characterization of glutamate dehydrogenase as another isoprotein binding to the membrane of rough endoplasmic reticulum (1999), J. Cell. Biochem., 76, 244-253.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.364
-
NAD+ mitochondrial enzyme Rattus norvegicus
0.443
-
NADP+ enzyme from rough ER Rattus norvegicus
0.637
-
NADP+ mitochondrial enzyme Rattus norvegicus
0.924
-
NAD+ enzyme from rough ER Rattus norvegicus
4.61
-
glutamate mitochondrial enzyme, cofactor NAD+ Rattus norvegicus
5.93
-
glutamate enzyme from rough ER, cofactor NAD+ Rattus norvegicus
20.7
-
glutamate mitochondrial enzyme, cofactor NADP+ Rattus norvegicus
23.8
-
glutamate enzyme from rough ER, cofactor NADP+ Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
rough endoplasmic reticulum peripheral membrane protein Rattus norvegicus 5791
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56000
-
x * 56000, enzymes from mitochondria and endoplasmic reticulum Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-Sepharose, ATP-agarose, Resource Q Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
29.7
-
-
Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoglutarate + NADH + NH3
-
Rattus norvegicus L-glutamate + NAD+ + H2O
-
?
L-glutamate + H2O + NAD(P)+
-
Rattus norvegicus 2-oxoglutarate + NH3 + NAD(P)H
-
r

Subunits

Subunits Comment Organism
? x * 56000, enzymes from mitochondria and endoplasmic reticulum Rattus norvegicus

Temperature Stability [┬░C]

Temperature Stability Minimum [┬░C] Temperature Stability Maximum [┬░C] Comment Organism
42
-
mitochondrial enzyme loses 80% activity after 20 min, enzyme from endoplasmic reticulum loses 20% activity Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
reductive amination Rattus norvegicus
9 9.5 glutamte deamination Rattus norvegicus

pH Range

pH Minimum pH Maximum Comment Organism
7.5 8 half activity of glutamate deamination compared to pH 9.5 Rattus norvegicus