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Literature summary for 1.4.1.3 extracted from

  • Heeschen, V.; Gerendas, J.; Richter, C.P.; Rudolph, H.
    Glutamate dehydrogenase of Sphagnum (1997), Phytochemistry, 45, 881-887.
No PubMed abstract available
Search for more literature for 1.4.1.3

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0083
-
 NADH
-
 Sphagnum fallax
0.011
-
 NADPH
-
 Sphagnum fallax
0.014
-
 NAD+
-
 Sphagnum fallax
0.7
-
 2-oxoglutarate cofactor NADH Sphagnum fallax
2.1
-
 glutamate
-
 Sphagnum fallax
6.3
-
 2-oxoglutarate cofactor NADPH Sphagnum fallax
28
-
 NH3 cofactor NADH Sphagnum fallax
41
-
 NH3 cofactor NADPH Sphagnum fallax

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Sphagnum fallax 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52000
-
 4 * 52000, SDS-PAGE Sphagnum fallax
210000
-
 gel filtration Sphagnum fallax

Organism

Organism UniProt Comment Textmining
Sphagnum fallax
-
 enhanced ammonium concentrations and a reduced carbon supply induce the enzyme activity
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, phenyl Sepharose, Superdex 200, Q Sepharose Sphagnum fallax

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
40.86
-
-
 Sphagnum fallax

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoglutarate + NH3 + NADH + H+
-
 Sphagnum fallax L-glutamate + H2O + NAD+
-
 r
2-oxoglutarate + NH3 + NADH + H+ NH4Cl used in enzyme assay Sphagnum fallax L-glutamate + H2O + NAD+
-
 r
L-glutamate + H2O + NAD(P)+
-
 Sphagnum fallax 2-oxoglutarate + NH3 + NAD(P)H
-
 r
L-glutamate + H2O + NAD+
-
 Sphagnum fallax 2-oxoglutarate + NH3 + NADH + H+
-
 r

Subunits

Subunits Comment Organism
tetramer 4 * 52000, SDS-PAGE Sphagnum fallax

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.7
-
 with NAD(P)H Sphagnum fallax
8.6
-
 with NAD+ Sphagnum fallax

Cofactor

Cofactor Comment Organism Structure
NAD+ no activity with NADP+ Sphagnum fallax
NADH ratio of NADH/NADPH-dependent activity: 1/1.6 Sphagnum fallax
NADPH
-
 Sphagnum fallax