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Literature summary for 1.4.1.3 extracted from

  • Robb, F.T.; Park, J.B.; Adams, M.W.W.
    Characterization of an extremely thermostable glutamate dehydrogenase: a key enzyme in the primary metabolism of the hyperthermophilic archaebacterium, Pyrococcus furiosus (1992), Biochim. Biophys. Acta, 1120, 267-272.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.18
-
NADP+ substrate L-glutamate Pyrococcus furiosus
0.53
-
NAD+ substrate L-glutamate Pyrococcus furiosus
0.56
-
NADPH substrate 2-oxoglutarate Pyrococcus furiosus
0.56
-
NADPH substrate NH3 Pyrococcus furiosus
0.98
-
NADH substrate 2-oxoglutarate Pyrococcus furiosus
1.6
-
L-glutamate cofactor NADP+ Pyrococcus furiosus
1.66
-
L-glutamate cofactor NAD+ Pyrococcus furiosus
4
-
2-oxoglutarate cofactor NADPH Pyrococcus furiosus
4.5
-
2-oxoglutarate cofactor NADH Pyrococcus furiosus
9.5
-
NH3 cofactor NADPH Pyrococcus furiosus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46000
-
6 * 46000, SDS-PAGE Pyrococcus furiosus
270000
-
gel filtration Pyrococcus furiosus

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Pyrococcus furiosus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
32.5
-
at 85┬░C Pyrococcus furiosus

Storage Stability

Storage Stability Organism
4┬░C, 6 months, stable Pyrococcus furiosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamate + H2O + NAD(P)+
-
Pyrococcus furiosus 2-oxoglutarate + NH3 + NAD(P)H
-
r

Subunits

Subunits Comment Organism
hexamer 6 * 46000, SDS-PAGE Pyrococcus furiosus

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
95
-
-
Pyrococcus furiosus

Temperature Stability [┬░C]

Temperature Stability Minimum [┬░C] Temperature Stability Maximum [┬░C] Comment Organism
100
-
half-life: 2.3 h at 0.053 mg/ml protein concentration, 10 h at 1.06 mg/ml protein concentration Pyrococcus furiosus

Cofactor

Cofactor Comment Organism Structure
NADP+ enzyme activity with NADP+ is higher than the activity with NAD+ Pyrococcus furiosus