KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.18 | - |
NADP+ | substrate L-glutamate | Pyrococcus furiosus | |
0.53 | - |
NAD+ | substrate L-glutamate | Pyrococcus furiosus | |
0.56 | - |
NADPH | substrate 2-oxoglutarate | Pyrococcus furiosus | |
0.56 | - |
NADPH | substrate NH3 | Pyrococcus furiosus | |
0.98 | - |
NADH | substrate 2-oxoglutarate | Pyrococcus furiosus | |
1.6 | - |
L-glutamate | cofactor NADP+ | Pyrococcus furiosus | |
1.66 | - |
L-glutamate | cofactor NAD+ | Pyrococcus furiosus | |
4 | - |
2-oxoglutarate | cofactor NADPH | Pyrococcus furiosus | |
4.5 | - |
2-oxoglutarate | cofactor NADH | Pyrococcus furiosus | |
9.5 | - |
NH3 | cofactor NADPH | Pyrococcus furiosus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46000 | - |
6 * 46000, SDS-PAGE | Pyrococcus furiosus |
270000 | - |
gel filtration | Pyrococcus furiosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pyrococcus furiosus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
32.5 | - |
at 85°C | Pyrococcus furiosus |
Storage Stability | Organism |
---|---|
4°C, 6 months, stable | Pyrococcus furiosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + H2O + NAD(P)+ | - |
Pyrococcus furiosus | 2-oxoglutarate + NH3 + NAD(P)H | - |
r |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 46000, SDS-PAGE | Pyrococcus furiosus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
95 | - |
- |
Pyrococcus furiosus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
100 | - |
half-life: 2.3 h at 0.053 mg/ml protein concentration, 10 h at 1.06 mg/ml protein concentration | Pyrococcus furiosus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | enzyme activity with NADP+ is higher than the activity with NAD+ | Pyrococcus furiosus |