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Literature summary for 1.4.1.3 extracted from

  • Yamamoto, I.; Saito, H.; Ishimoto, M.
    Comparison of properties of glutamate dehydrogenases in members of the Bacteroides fragilis group (1987), J. Gen. Appl. Microbiol., 33, 429-436.
No PubMed abstract available

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.006
-
NADPH NADPH-dependent amination Phocaeicola vulgatus
0.018
-
NADPH NADPH-dependent amination Parabacteroides distasonis
0.02
-
NADPH NADPH-dependent amination Bacteroides ovatus
0.63
-
2-oxoglutarate NADPH-dependent amination Phocaeicola vulgatus
0.64
-
2-oxoglutarate NADPH-dependent amination Bacteroides ovatus
0.7
-
2-oxoglutarate NADPH-dependent amination Parabacteroides distasonis
1.5
-
NH3 NADPH-dependent amination Phocaeicola vulgatus
2.4
-
NH3 NADPH-dependent amination Parabacteroides distasonis
2.9
-
NH3 NADPH-dependent amination Bacteroides ovatus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-glutamate + H2O + NAD(P)+ Bacteroides fragilis enzyme for the main route for ammonia assimilation at low concentrations of ammonia 2-oxoglutarate + NH3 + NAD(P)H
-
r

Organism

Organism UniProt Comment Textmining
Bacteroides fragilis
-
-
-
Bacteroides ovatus
-
-
-
Parabacteroides distasonis
-
-
-
Phocaeicola vulgatus
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [Āµmol/min/mg] Specific Activity Maximum [Āµmol/min/mg] Comment Organism
0.168
-
NADH dependent activity in cells grown on medium containing 1 mM NH4Cl Bacteroides ovatus
0.257
-
NADH dependent activity in cells grown on medium containing 1 mM NH4Cl Parabacteroides distasonis
0.336
-
NADH dependent activity in cells grown on medium containing 1 mM NH4Cl Bacteroides ovatus
0.858
-
NADPH dependent activity in cells grown on medium containing 1 mM NH4Cl Bacteroides ovatus
1.07
-
NADH dependent activity in cells grown on medium containing 1 mM NH4Cl Bacteroides fragilis
1.96
-
NADPH dependent activity in cells grown on medium containing 1 mM NH4Cl Parabacteroides distasonis
2.22
-
NADPH dependent activity in cells grown on medium containing 1 mM NH4Cl Bacteroides ovatus
5.18
-
NADPH dependent activity in cells grown on medium containing 1 mM NH4Cl Bacteroides fragilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoglutarate + NH3 + NADH + H+ NH4Cl used in enzyme assay Parabacteroides distasonis L-glutamate + H2O + NAD+
-
r
2-oxoglutarate + NH3 + NADH + H+ NH4Cl used in enzyme assay Bacteroides ovatus L-glutamate + H2O + NAD+
-
r
2-oxoglutarate + NH3 + NADH + H+ NH4Cl used in enzyme assay Phocaeicola vulgatus L-glutamate + H2O + NAD+
-
r
L-glutamate + H2O + NAD(P)+
-
Bacteroides fragilis 2-oxoglutarate + NH3 + NAD(P)H
-
r
L-glutamate + H2O + NAD(P)+
-
Parabacteroides distasonis 2-oxoglutarate + NH3 + NAD(P)H
-
r
L-glutamate + H2O + NAD(P)+
-
Bacteroides ovatus 2-oxoglutarate + NH3 + NAD(P)H
-
r
L-glutamate + H2O + NAD(P)+
-
Phocaeicola vulgatus 2-oxoglutarate + NH3 + NAD(P)H
-
r
L-glutamate + H2O + NAD(P)+ enzyme for the main route for ammonia assimilation at low concentrations of ammonia Bacteroides fragilis 2-oxoglutarate + NH3 + NAD(P)H
-
r

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.95
-
NADPH-dependent amination Parabacteroides distasonis
7.95
-
NADPH-dependent amination Bacteroides ovatus
8.05
-
NADPH-dependent amination Phocaeicola vulgatus