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Literature summary for 1.4.1.3 extracted from

  • Mc Kenzie, E.A.; Copeland, L.; Lees, E.M.
    Glutamate dehydrogenase activity in developing soybean seed: kinetic properties of three forms of the enzyme (1981), Arch. Biochem. Biophys., 212, 298-305.
    View publication on PubMed
Search for more literature for 1.4.1.3

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.009
-
 NADH glutamate dehydrogenase 1, 5 mM 2-oxoglutarate Glycine max
0.009
-
 NADH glutamate dehydrogenase 2, 5 mM 2-oxoglutarate Glycine max
0.012
-
 NADH glutamate dehydrogenase 2, 80 mM NH4+ Glycine max
0.02
-
 NADH glutamate dehydrogenase 1, 40 mM NH4+ Glycine max
0.028
-
 NADPH glutamate dehydrogenase 1, 10 mM 2-oxoglutarate Glycine max
0.028
-
 NADPH glutamate dehydrogenase 2, 10 mM 2-oxoglutarate Glycine max
0.048
-
 NADH glutamate dehydrogenase 1, 400 mM NH4+ Glycine max
0.05
-
 NADH glutamate dehydrogenase 1, 12.5 mM 2-oxoglutarate Glycine max
0.069
-
 NADPH glutamate dehydrogenase 1, 40 mM NH4+ Glycine max
0.069
-
 NADPH glutamate dehydrogenase 2, 80 mM NH4+ Glycine max
0.62
-
 2-oxoglutarate glutamate dehydrogenase 2, 0.1 mM NADH Glycine max
1.1
-
 2-oxoglutarate glutamate dehydrogenase 2, 80 mM NH4+ Glycine max
1.51
-
 2-oxoglutarate glutamate dehydrogenase 1, 400 mM NH4+ Glycine max
1.51
-
 2-oxoglutarate glutamate dehydrogenase 1, 0.1 mM NADH Glycine max
2.1
-
 2-oxoglutarate glutamate dehydrogenase 1, 40 mM NH4+ Glycine max
2.6
-
 2-oxoglutarate glutamate dehydrogenase 1, 0.2 mM NADPH Glycine max
3.7
-
 2-oxoglutarate glutamate dehydrogenase 1, 0.2 mM NADH Glycine max
3.7
-
 2-oxoglutarate glutamate dehydrogenase 2, 0.2 mM NADPH Glycine max
5.8
-
 NH4+ glutamate dehydrogenase 1, 0.2 mM NADPH Glycine max
7
-
 NH4+ glutamate dehydrogenase 1, 0.2 mM NADPH Glycine max
10.4
-
 NH4+ glutamate dehydrogenase 1, 10 mM 2-oxoglutarate Glycine max
12.9
-
 NH4+ glutamate dehydrogenase 2, 5 mM 2-oxoglutarate Glycine max
15.8
-
 NH4+ glutamate dehydrogenase 1, 5 mM 2-oxoglutarate Glycine max
19
-
 NH4+ glutamate dehydrogenase 1, 10 mM 2-oxoglutarate Glycine max
21.7
-
 NH4+ glutamate dehydrogenase 1, 0.1 mM NADH Glycine max
22.8
-
 NH4+ glutamate dehydrogenase 1, 0.1 mM NADH Glycine max
106
-
 NH4+ glutamate dehydrogenase 1, 12.5 mM 2-oxoglutarate Glycine max
115.1
-
 NH4+ glutamate dehydrogenase 1, 0.2 mM NADH Glycine max

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast glutamate dehydrogenase 2 Glycine max 9507
-

Organism

Organism UniProt Comment Textmining
Glycine max
-
 glutamate dehydrogenase 1 and 2
-

Source Tissue

Source Tissue Comment Organism Textmining
seed
-
 Glycine max
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoglutarate + NH3 + NADH + H+
-
 Glycine max L-glutamate + H2O + NAD+
-
 r
L-glutamate + H2O + NAD(P)+
-
 Glycine max 2-oxoglutarate + NH3 + NAD(P)H
-
 r

Cofactor

Cofactor Comment Organism Structure
additional information 2 soybean isoenzymes with different coenzyme specificities, glutamate dehydrogenase 1: active with NADH, NAD+, NADPH and NADP+, glutamate dehydrogenase 2: active with NADH, NAD+, NADPH but not NADP+ Glycine max
NAD+ glutamate dehydrogenase 2 uses only NAD+ in the deamination direction Glycine max
NADP+ with NADP+ initial rates are much slower than with NAD+ Glycine max