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Literature summary for 1.4.1.28 extracted from

  • Mayol, O.; Bastard, K.; Beloti, L.; Frese, A.; Turkenburg, J.; Petit, J.; Mariage, A.; Debard, A.; Pellouin, V.; Perret, A.; de Berardinis, V.; Zaparucha, A.; Grogan, G.; Vergne-Vaxelaire, C.
    A family of native amine dehydrogenases for the asymmetric reductive amination of ketones (2019), Nat. Catal., 2, 324-333 .
No PubMed abstract available
Search for more literature for 1.4.1.28

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
 NADPH pH 9.5, 23°C Cystobacter fuscus
0.06
-
 NADPH pH 9.5, 23°C Mycolicibacterium smegmatis
0.06
-
 NADH pH 9.5, 23°C Cystobacter fuscus
0.29
-
 NADH pH 9.5, 23°C Mycolicibacterium smegmatis
0.55
-
 cyclohexanone pH 9.5, 23°C Mycolicibacterium smegmatis
2.32
-
 2-methylcyclohexanone pH 9.5, 23°C Mycolicibacterium smegmatis
2.5
-
 cyclohexanone pH 9.5, 23°C Cystobacter fuscus
98.9
-
 NH3 pH 9.5, 23°C Cystobacter fuscus
392
-
 NH3 pH 9.5, 23°C Mycolicibacterium smegmatis

Organism

Organism UniProt Comment Textmining
Cystobacter fuscus S9Q235
-
-
Cystobacter fuscus DSM 2262 S9Q235
-
-
Microbacterium sp. MA1 C3UMY1
-
-
Mycolicibacterium smegmatis A0A8B4R7U8
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3
-
 substrate 2-cyclohexen-1-one, cosubstrate NADH, pH 9.5, 23°C Mycolicibacterium smegmatis
2.3
-
 substrate cyclopentanone, cosubstrate NADH, pH 9.5, 23°C Mycolicibacterium smegmatis
2.7
-
 substrate 3-buten-2-one, cosubstrate NADH, pH 9.5, 23°C Mycolicibacterium smegmatis
3 8 substrate 3-methylcyclohexanone, cosubstrate NADH, pH 9.5, 23°C Mycolicibacterium smegmatis
3.6
-
 substrate 2-cyclohexen-1-one, cosubstrate NADPH, pH 9.5, 23°C Cystobacter fuscus
4.3
-
 substrate 2-cyclohexen-1-one, cosubstrate NADH, pH 9.5, 23°C Cystobacter fuscus
5.6
-
 substrate cyclopentanone, cosubstrate NADPH, pH 9.5, 23°C Mycolicibacterium smegmatis
7.4
-
 substrate 2-cyclohexen-1-one, cosubstrate NADPH, pH 9.5, 23°C Mycolicibacterium smegmatis
7.9
-
 substrate 3-buten-2-one, cosubstrate NADPH, pH 9.5, 23°C Mycolicibacterium smegmatis
9.3
-
 substrate 4-methylcyclohexanone, cosubstrate NADH, pH 9.5, 23°C Mycolicibacterium smegmatis
14.9
-
 substrate cyclopentanone, cosubstrate NADH, pH 9.5, 23°C Microbacterium sp. MA1
18.6
-
 substrate 3-methylbutan-2-one, cosubstrate NADH, pH 9.5, 23°C Mycolicibacterium smegmatis
18.8
-
 substrate 4-methylcyclohexanone, cosubstrate NADH, pH 9.5, 23°C Cystobacter fuscus
19.6
-
 substrate 2-methylcyclohexanone, cosubstrate NADH, pH 9.5, 23°C Cystobacter fuscus
25.1
-
 substrate cyclohexanone, cosubstrate NADPH, pH 9.5, 23°C Mycolicibacterium smegmatis
25.5
-
 substrate 2-methylcyclohexanone, cosubstrate NADPH, pH 9.5, 23°C Cystobacter fuscus
33.8
-
 substrate 3-methylcyclohexanone, cosubstrate NADH, pH 9.5, 23°C Cystobacter fuscus
34.2
-
 substrate 2-cyclohexen-1-one, cosubstrate NADH, pH 9.5, 23°C Microbacterium sp. MA1
35
-
 substrate 4-methylcyclohexanone, cosubstrate NADPH, pH 9.5, 23°C Cystobacter fuscus
37.1
-
 substrate 2-cyclohexen-1-one, cosubstrate NADPH, pH 9.5, 23°C Microbacterium sp. MA1
37.3
-
 substrate 2-methylcyclohexanone, cosubstrate NADH, pH 9.5, 23°C Mycolicibacterium smegmatis
40.1
-
 substrate cyclopentanone, cosubstrate NADH, pH 9.5, 23°C Cystobacter fuscus
41.5
-
 substrate cyclopentanone, cosubstrate NADPH, pH 9.5, 23°C Cystobacter fuscus
54.4
-
 substrate 3-methylbutan-2-one, cosubstrate NADH, pH 9.5, 23°C Microbacterium sp. MA1
60.1
-
 substrate 4-methylcyclohexanone, cosubstrate NADH, pH 9.5, 23°C Microbacterium sp. MA1
60.2
-
 substrate cyclohexanone, cosubstrate NADPH, pH 9.5, 23°C Cystobacter fuscus
60.6
-
 substrate cyclopentanone, cosubstrate NADPH, pH 9.5, 23°C Microbacterium sp. MA1
63.6
-
 substrate 3-methylcyclohexanone, cosubstrate NADPH, pH 9.5, 23°C Cystobacter fuscus
85.2
-
 substrate 4-methylcyclohexanone, cosubstrate NADPH, pH 9.5, 23°C Mycolicibacterium smegmatis
109.6
-
 substrate 3-methylbutan-2-one, cosubstrate NADPH, pH 9.5, 23°C Cystobacter fuscus
120.6
-
 substrate 3-methylbutan-2-one, cosubstrate NADH, pH 9.5, 23°C Cystobacter fuscus
129
-
 substrate 3-methylbutan-2-one, cosubstrate NADPH, pH 9.5, 23°C Mycolicibacterium smegmatis
160.9
-
 substrate 3-methylcyclohexanone, cosubstrate NADPH, pH 9.5, 23°C Mycolicibacterium smegmatis
161
-
 substrate 3-methylcyclohexanone, cosubstrate NADH, pH 9.5, 23°C Microbacterium sp. MA1
170.1
-
 substrate 4-methylcyclohexanone, cosubstrate NADPH, pH 9.5, 23°C Microbacterium sp. MA1
175.2
-
 substrate 2-methylcyclohexanone, cosubstrate NADPH, pH 9.5, 23°C Mycolicibacterium smegmatis
196.2
-
 substrate cyclohexanone, cosubstrate NADH, pH 9.5, 23°C Mycolicibacterium smegmatis
206.5
-
 substrate 3-methylcyclohexanone, cosubstrate NADPH, pH 9.5, 23°C Microbacterium sp. MA1
233.2
-
 substrate cyclohexanone, cosubstrate NADH, pH 9.5, 23°C Cystobacter fuscus
245.6
-
 substrate 3-methylbutan-2-one, cosubstrate NADPH, pH 9.5, 23°C Microbacterium sp. MA1
252.6
-
 substrate 2-methylcyclohexanone, cosubstrate NADPH, pH 9.5, 23°C Microbacterium sp. MA1
337
-
 substrate 2-methylcyclohexanone, cosubstrate NADH, pH 9.5, 23°C Microbacterium sp. MA1
389
-
 substrate cyclohexanone, cosubstrate NADPH, pH 9.5, 23°C Microbacterium sp. MA1
614
-
 substrate cyclohexanone, cosubstrate NADH, pH 9.5, 23°C Microbacterium sp. MA1

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-cyclohexen-1-one + NH3 + NADH + H+
-
 Mycolicibacterium smegmatis 2-cyclohexen-1-amine + H2O + NAD+
-
 ?
2-cyclohexen-1-one + NH3 + NADH + H+
-
 Cystobacter fuscus 2-cyclohexen-1-amine + H2O + NAD+
-
 ?
2-cyclohexen-1-one + NH3 + NADH + H+
-
 Microbacterium sp. MA1 2-cyclohexen-1-amine + H2O + NAD+
-
 ?
2-cyclohexen-1-one + NH3 + NADH + H+
-
 Cystobacter fuscus DSM 2262 2-cyclohexen-1-amine + H2O + NAD+
-
 ?
2-methylcyclohexanone + NH3 + NADH + H+
-
 Mycolicibacterium smegmatis 2-methylcyclohexylamine + H2O + NAD+
-
 ?
2-methylcyclohexanone + NH3 + NADH + H+
-
 Cystobacter fuscus 2-methylcyclohexylamine + H2O + NAD+
-
 ?
2-methylcyclohexanone + NH3 + NADH + H+
-
 Microbacterium sp. MA1 2-methylcyclohexylamine + H2O + NAD+
-
 ?
2-methylcyclohexanone + NH3 + NADH + H+
-
 Cystobacter fuscus DSM 2262 2-methylcyclohexylamine + H2O + NAD+
-
 ?
3-buten-2-one + NH3 + NADH + H+
-
 Mycolicibacterium smegmatis 3-buten-2-amine + H2O + NAD+
-
 ?
3-methylbutan-2-one + NH3 + NADH + H+
-
 Mycolicibacterium smegmatis 3-methylbutan-2-amine + H2O + NAD+
-
 ?
3-methylbutan-2-one + NH3 + NADH + H+
-
 Cystobacter fuscus 3-methylbutan-2-amine + H2O + NAD+
-
 ?
3-methylbutan-2-one + NH3 + NADH + H+
-
 Microbacterium sp. MA1 3-methylbutan-2-amine + H2O + NAD+
-
 ?
3-methylbutan-2-one + NH3 + NADH + H+
-
 Cystobacter fuscus DSM 2262 3-methylbutan-2-amine + H2O + NAD+
-
 ?
3-methylcyclohexanone + NH3 + NADH + H+
-
 Mycolicibacterium smegmatis 3-methylcyclohexylamine + H2O + NAD+
-
 ?
3-methylcyclohexanone + NH3 + NADH + H+
-
 Cystobacter fuscus 3-methylcyclohexylamine + H2O + NAD+
-
 ?
3-methylcyclohexanone + NH3 + NADH + H+
-
 Microbacterium sp. MA1 3-methylcyclohexylamine + H2O + NAD+
-
 ?
4-methylcyclohexanone + NH3 + NADH + H+
-
 Mycolicibacterium smegmatis 4-methylcyclohexylamine + H2O + NAD+
-
 ?
4-methylcyclohexanone + NH3 + NADH + H+
-
 Cystobacter fuscus 4-methylcyclohexylamine + H2O + NAD+
-
 ?
4-methylcyclohexanone + NH3 + NADH + H+
-
 Microbacterium sp. MA1 4-methylcyclohexylamine + H2O + NAD+
-
 ?
cyclohexanone + methylamine + NADPH + H+
-
 Mycolicibacterium smegmatis N-methylcyclohexanamine + H2O + NADP+
-
 ?
cyclohexanone + methylamine + NADPH + H+
-
 Cystobacter fuscus N-methylcyclohexanamine + H2O + NADP+
-
 ?
cyclohexanone + methylamine + NADPH + H+
-
 Microbacterium sp. MA1 N-methylcyclohexanamine + H2O + NADP+
-
 ?
cyclopentanone + NH3 + NADH + H+
-
 Mycolicibacterium smegmatis cyclopentylamine + H2O + NAD+
-
 ?
cyclopentanone + NH3 + NADH + H+
-
 Cystobacter fuscus cyclopentylamine + H2O + NAD+
-
 ?
cyclopentanone + NH3 + NADH + H+
-
 Microbacterium sp. MA1 cyclopentylamine + H2O + NAD+
-
 ?
cyclopentanone + NH3 + NADH + H+
-
 Cystobacter fuscus DSM 2262 cyclopentylamine + H2O + NAD+
-
 ?
additional information also reacts with aldehydes such as 2-methylbutanal, isobutyraldehyde Mycolicibacterium smegmatis ?
-
-
additional information also reacts with aldehydes such as 2-methylbutanal, isobutyraldehyde Cystobacter fuscus ?
-
-
additional information also reacts with aldehydes such as 2-methylbutanal, isobutyraldehyde Microbacterium sp. MA1 ?
-
-
additional information also reacts with aldehydes such as 2-methylbutanal, isobutyraldehyde Cystobacter fuscus DSM 2262 ?
-
-

Synonyms

Synonyms Comment Organism
AMDH
-
 Mycolicibacterium smegmatis
AMDH
-
 Cystobacter fuscus
AMDH
-
 Microbacterium sp. MA1

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.04
-
 NH3 pH 9.5, 23°C Cystobacter fuscus
0.05
-
 NADPH pH 9.5, 23°C Cystobacter fuscus
0.1
-
 NADH pH 9.5, 23°C Cystobacter fuscus
0.28
-
 NADPH pH 9.5, 23°C Mycolicibacterium smegmatis
0.3
-
 NH3 pH 9.5, 23°C Mycolicibacterium smegmatis
0.58
-
 NADH pH 9.5, 23°C Mycolicibacterium smegmatis
0.61
-
 2-methylcyclohexanone pH 9.5, 23°C Mycolicibacterium smegmatis
0.94
-
 cyclohexanone pH 9.5, 23°C Mycolicibacterium smegmatis
13
-
 cyclohexanone pH 9.5, 23°C Cystobacter fuscus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0008
-
 NH3 pH 9.5, 23°C Mycolicibacterium smegmatis
0.261
-
 2-methylcyclohexanone pH 9.5, 23°C Mycolicibacterium smegmatis
0.4
-
 NH3 pH 9.5, 23°C Cystobacter fuscus
1.723
-
 cyclohexanone pH 9.5, 23°C Mycolicibacterium smegmatis
1.995
-
 NADH pH 9.5, 23°C Mycolicibacterium smegmatis
4.893
-
 NADPH pH 9.5, 23°C Mycolicibacterium smegmatis
54.4
-
 cyclohexanone pH 9.5, 23°C Cystobacter fuscus
1704
-
 NADH pH 9.5, 23°C Cystobacter fuscus
4537
-
 NADPH pH 9.5, 23°C Cystobacter fuscus