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Literature summary for 1.4.1.28 extracted from

  • Mayol, O.; Bastard, K.; Beloti, L.; Frese, A.; Turkenburg, J.P.; Petit, J.-L.; Mariage, A.; Debard, A.; Pellouin, V.; Perret, A.; de Berardinis, V.; Zaparucha, A.; Grogan, G.; Vergne-Vaxelaire, C.
    A family of native amine dehydrogenases for the asymmetric reductive amination of ketones (2019), Nat. Catal., 2, 324 .
No PubMed abstract available
Search for more literature for 1.4.1.28

Crystallization (Commentary)

Crystallization (Comment) Organism
structure without substrate but bound to the cofactor NAD+, and a model of the ternary complex with product (2R,4S)-2,4-diaminopentanoate in the closed conformation. The active site features residues Glu102, Val134, Asn135, Arg161, Asn163, Phe168, Val172, Gln176, His197, Ile198, His264, Gln266, Gly299 and Thr303 Petrotoga mobilis

Protein Variants

Protein Variants Comment Organism
E102A residue Glu102 is involved in the activation of ammonia, mutation significantly decreases activity Petrotoga mobilis
R161M mutant gains activity with substrate pentan-2-one Petrotoga mobilis

Inhibitors

Inhibitors Comment Organism Structure
2-methylcyclohexanone
-
 Microbacterium sp. MA1
2-methylcyclohexanone
-
 Mycolicibacterium smegmatis
2-methylcyclohexanone
-
 Petrotoga mobilis
cyclohexanone substrate inhibition Cystobacter fuscus
cyclohexanone
-
 Microbacterium sp. MA1
cyclohexanone
-
 Mycolicibacterium smegmatis
cyclohexanone
-
 Petrotoga mobilis
Isobutanal
-
 Microbacterium sp. MA1
Isobutanal
-
 Mycolicibacterium smegmatis
Isobutanal
-
 Petrotoga mobilis
NH3 substrate inhibition Cystobacter fuscus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
 NADPH pH 9.5, 23°C Cystobacter fuscus
0.025
-
 NADH wild-type, pH 9.5, 23°C Petrotoga mobilis
0.06
-
 NADPH pH 9.5, 23°C Mycolicibacterium smegmatis
0.06
-
 NADH pH 9.5, 23°C Cystobacter fuscus
0.29
-
 NADH pH 9.5, 23°C Mycolicibacterium smegmatis
0.326
-
 (2R)-2-amino-4-oxopentanoate wild-type, pH 9.5, 23°C Petrotoga mobilis
0.54
-
 Isobutanal pH 9.5, 23°C Cystobacter fuscus
0.55
-
 cyclohexanone pH 9.5, 23°C Mycolicibacterium smegmatis
0.86
-
 Isobutanal pH 9.5, 23°C Mycolicibacterium smegmatis
0.894
-
 (2R)-2-amino-4-oxopentanoate mutant E102A, pH 9.5, 23°C Petrotoga mobilis
2.32
-
 2-methylcyclohexanone pH 9.5, 23°C Mycolicibacterium smegmatis
2.47
-
 cyclohexanone pH 9.5, 23°C Cystobacter fuscus
98.94
-
 NH3 pH 9.5, 23°C Cystobacter fuscus
319.7
-
 NH3 wild-type, pH 9.5, 23°C Petrotoga mobilis
392.2
-
 NH3 pH 9.5, 23°C Mycolicibacterium smegmatis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(2R)-2-amino-4-oxopentanoate + NH3 + NADH + H+ Petrotoga mobilis
-
 (2R,4S)-2,4-diaminopentanoate + NAD+ + H2O
-
 ?
(2R)-2-amino-4-oxopentanoate + NH3 + NADH + H+ Petrotoga mobilis DSM 10674
-
 (2R,4S)-2,4-diaminopentanoate + NAD+ + H2O
-
 ?
pentan-2-one + NH3 + NADH + H+ Petrotoga mobilis no substrate of wild-type, but substrate of mutants carrying substitution R161M pentan-2-amine + NAD+ + H2O
-
 ?
pentan-2-one + NH3 + NADH + H+ Petrotoga mobilis DSM 10674 no substrate of wild-type, but substrate of mutants carrying substitution R161M pentan-2-amine + NAD+ + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Cystobacter fuscus S9Q235
-
-
Cystobacter fuscus DSM 2262 S9Q235
-
-
Microbacterium sp. MA1 C3UMY1
-
-
Mycolicibacterium smegmatis A0A8B4R7U8
-
-
Petrotoga mobilis A9BHL2
-
-
Petrotoga mobilis DSM 10674 A9BHL2
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
10.1
-
 substrate cyclohexanone, cosubstrate methylamine, pH 9.5, 23°C Mycolicibacterium smegmatis
16.4
-
 substrate 2-methylbutanal, cosubstrates NH3 and NADPH, pH 9.5, 23°C Mycolicibacterium smegmatis
18.6
-
 substrate 3-methylbutan-2-one, cosubstrates NH3 and NADPH, pH 9.5, 23°C Mycolicibacterium smegmatis
19.6
-
 substrate 2-methylcyclohexanone, cosubstrates NH3 and NADPH, pH 9.5, 23°C Cystobacter fuscus
25.1
-
 substrate cyclohexanone, cosubstrates NH3 and NADPH, pH 9.5, 23°C Mycolicibacterium smegmatis
25.5
-
 substrate 2-methylcyclohexanone, cosubstrates NH3 and NADPH, pH 9.5, 23°C Cystobacter fuscus
37.3
-
 substrate 2-methylcyclohexanone, cosubstrates NH3 and NADPH, pH 9.5, 23°C Mycolicibacterium smegmatis
54.4
-
 substrate 3-methylbutan-2-one, cosubstrates NH3 and NADPH, pH 9.5, 23°C Microbacterium sp. MA1
60.2
-
 substrate cyclohexanone, cosubstrates NH3 and NADPH, pH 9.5, 23°C Cystobacter fuscus
73.8
-
 substrate isobutanal, cosubstrates NH3 and NADPH, pH 9.5, 23°C Cystobacter fuscus
75.2
-
 substrate 2-methylbutanal, cosubstrates NH3 and NADPH, pH 9.5, 23°C Mycolicibacterium smegmatis
75.2
-
 substrate 2-methylbutanal, cosubstrates NH3 and NADPH, pH 9.5, 23°C Microbacterium sp. MA1
88.1
-
 substrate isobutanal, cosubstrates NH3 and NADPH, pH 9.5, 23°C Mycolicibacterium smegmatis
88.1
-
 substrate isobutanal, cosubstrates NH3 and NADPH, pH 9.5, 23°C Microbacterium sp. MA1
90.4
-
 substrate cyclohexanone, cosubstrate methylamine, pH 9.5, 23°C Microbacterium sp. MA1
97.6
-
 substrate 2-methylbutanal, cosubstrates NH3 and NADPH, pH 9.5, 23°C Microbacterium sp. MA1
104.8
-
 mutant N135V/N163V/R161M/H264L, substrate pentan-2-one, 23°C, pH 9.5 Petrotoga mobilis
109.6
-
 substrate 3-methylbutan-2-one, cosubstrates NH3 and NADPH, pH 9.5, 23°C Cystobacter fuscus
119.2
-
 substrate isobutanal, cosubstrates NH3 and NADPH, pH 9.5, 23°C Mycolicibacterium smegmatis
120.6
-
 substrate 3-methylbutan-2-one, cosubstrates NH3 and NADPH, pH 9.5, 23°C Cystobacter fuscus
129
-
 substrate 3-methylbutan-2-one, cosubstrates NH3 and NADPH, pH 9.5, 23°C Mycolicibacterium smegmatis
131.6
-
 substrate 2-methylbutanal, cosubstrates NH3 and NADPH, pH 9.5, 23°C Cystobacter fuscus
144.5
-
 substrate isobutanal, cosubstrate methylamine, pH 9.5, 23°C Mycolicibacterium smegmatis
159.4
-
 substrate isobutanal, cosubstrates NH3 and NADPH, pH 9.5, 23°C Microbacterium sp. MA1
175.2
-
 substrate 2-methylcyclohexanone, cosubstrates NH3 andNADPH, pH 9.5, 23°C Mycolicibacterium smegmatis
196.2
-
 substrate cyclohexanone, cosubstrates NH3 and NADH, pH 9.5, 23°C Mycolicibacterium smegmatis
233.2
-
 substrate cyclohexanone, cosubstrates NH3 and NADH, pH 9.5, 23°C Cystobacter fuscus
245.6
-
 substrate 3-methylbutan-2-one, cosubstrates NH3 and NADPH, pH 9.5, 23°C Microbacterium sp. MA1
252.6
-
 substrate 2-methylcyclohexanone, cosubstrates NH3 and NADPH, pH 9.5, 23°C Microbacterium sp. MA1
257.7
-
 substrate isobutanal, cosubstrate methylamine, pH 9.5, 23°C Cystobacter fuscus
337
-
 substrate 2-methylcyclohexanone, cosubstrates NH3 and NADPH, pH 9.5, 23°C Microbacterium sp. MA1
388.6
-
 substrate cyclohexanone, cosubstrates NH3 and NADPH, pH 9.5, 23°C Microbacterium sp. MA1
397.9
-
 substrate 2-methylbutanal, cosubstrates NH3 and NADPH, pH 9.5, 23°C Cystobacter fuscus
614.5
-
 substrate cyclohexanone, cosubstrates NH3 and NADH, pH 9.5, 23°C Microbacterium sp. MA1
851.3
-
 substrate isobutanal, cosubstrate methylamine, pH 9.5, 23°C Microbacterium sp. MA1
955
-
 substrate isobutanal, cosubstrates NH3 and NADPH, pH 9.5, 23°C Cystobacter fuscus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2R)-2-amino-4-oxopentanoate + NH3 + NADH + H+
-
 Petrotoga mobilis (2R,4S)-2,4-diaminopentanoate + NAD+ + H2O
-
 ?
(2R)-2-amino-4-oxopentanoate + NH3 + NADH + H+
-
 Petrotoga mobilis DSM 10674 (2R,4S)-2,4-diaminopentanoate + NAD+ + H2O
-
 ?
1-cyclohexylethan-1-one + NH3 + NADH + H+
-
 Mycolicibacterium smegmatis 1-cyclohexylethyl-1-amine + NAD+ + H2O
-
 ?
1-cyclohexylethan-1-one + NH3 + NADH + H+
-
 Cystobacter fuscus 1-cyclohexylethyl-1-amine + NAD+ + H2O
-
 ?
1-cyclohexylethan-1-one + NH3 + NADH + H+
-
 Microbacterium sp. MA1 1-cyclohexylethyl-1-amine + NAD+ + H2O
-
 ?
1-cyclohexylethan-1-one + NH3 + NADH + H+
-
 Cystobacter fuscus DSM 2262 1-cyclohexylethyl-1-amine + NAD+ + H2O
-
 ?
1-indanone + NH3 + NADH + H+
-
 Mycolicibacterium smegmatis 1-indanamine + NAD+ + H2O
-
 ?
1-indanone + NH3 + NADH + H+
-
 Cystobacter fuscus 1-indanamine + NAD+ + H2O
-
 ?
1-indanone + NH3 + NADH + H+
-
 Microbacterium sp. MA1 1-indanamine + NAD+ + H2O
-
 ?
1-indanone + NH3 + NADH + H+
-
 Cystobacter fuscus DSM 2262 1-indanamine + NAD+ + H2O
-
 ?
2-cyclohexen-1-one + NH3 + NADH + H+
-
 Mycolicibacterium smegmatis 2-cyclohexen-1-amine + NAD+ + H2O
-
 ?
2-cyclohexen-1-one + NH3 + NADH + H+
-
 Cystobacter fuscus 2-cyclohexen-1-amine + NAD+ + H2O
-
 ?
2-cyclohexen-1-one + NH3 + NADH + H+
-
 Microbacterium sp. MA1 2-cyclohexen-1-amine + NAD+ + H2O
-
 ?
2-cyclohexen-1-one + NH3 + NADH + H+
-
 Cystobacter fuscus DSM 2262 2-cyclohexen-1-amine + NAD+ + H2O
-
 ?
2-methylbutanal + NH3 + NADH + H+
-
 Mycolicibacterium smegmatis 2-methylbutan-1-amine + NAD+ + H2O
-
 ?
2-methylbutanal + NH3 + NADH + H+
-
 Cystobacter fuscus 2-methylbutan-1-amine + NAD+ + H2O
-
 ?
2-methylbutanal + NH3 + NADH + H+
-
 Microbacterium sp. MA1 2-methylbutan-1-amine + NAD+ + H2O
-
 ?
2-methylbutanal + NH3 + NADH + H+
-
 Cystobacter fuscus DSM 2262 2-methylbutan-1-amine + NAD+ + H2O
-
 ?
2-methylcyclohexanone + NH3 + NADH + H+
-
 Mycolicibacterium smegmatis 2-methylcyclohexylamine + NAD+ + H2O
-
 ?
2-methylcyclohexanone + NH3 + NADH + H+
-
 Cystobacter fuscus 2-methylcyclohexylamine + NAD+ + H2O
-
 ?
2-methylcyclohexanone + NH3 + NADH + H+
-
 Microbacterium sp. MA1 2-methylcyclohexylamine + NAD+ + H2O
-
 ?
2-methylcyclopentanone + NH3 + NADH + H+
-
 Mycolicibacterium smegmatis 2-methylcyclopentylamine + NAD+ + H2O
-
 ?
2-methylcyclopentanone + NH3 + NADH + H+
-
 Cystobacter fuscus 2-methylcyclopentylamine + NAD+ + H2O
-
 ?
2-methylcyclopentanone + NH3 + NADH + H+
-
 Microbacterium sp. MA1 2-methylcyclopentylamine + NAD+ + H2O
-
 ?
3,5-dimethylcyclohexanone + NH3 + NADH + H+
-
 Mycolicibacterium smegmatis 3,5-dimethylcyclohexylamine + NAD+ + H2O
-
 ?
3,5-dimethylcyclohexanone + NH3 + NADH + H+
-
 Cystobacter fuscus 3,5-dimethylcyclohexylamine + NAD+ + H2O
-
 ?
3,5-dimethylcyclohexanone + NH3 + NADH + H+
-
 Microbacterium sp. MA1 3,5-dimethylcyclohexylamine + NAD+ + H2O
-
 ?
3-hydroxybutan-2-one + NH3 + NADH + H+
-
 Mycolicibacterium smegmatis 3-aminobutan-2-ol + NAD+ + H2O
-
 ?
3-hydroxybutan-2-one + NH3 + NADH + H+
-
 Cystobacter fuscus 3-aminobutan-2-ol + NAD+ + H2O
-
 ?
3-hydroxybutan-2-one + NH3 + NADH + H+
-
 Microbacterium sp. MA1 3-aminobutan-2-ol + NAD+ + H2O
-
 ?
3-methylbutan-2-one + NH3 + NADH + H+
-
 Mycolicibacterium smegmatis 3-methylbutan-2-amine + NAD+ + H2O
-
 ?
3-methylbutan-2-one + NH3 + NADH + H+
-
 Cystobacter fuscus 3-methylbutan-2-amine + NAD+ + H2O
-
 ?
3-methylbutan-2-one + NH3 + NADH + H+
-
 Microbacterium sp. MA1 3-methylbutan-2-amine + NAD+ + H2O
-
 ?
3-methylcyclohexanone + NH3 + NADH + H+
-
 Mycolicibacterium smegmatis 3-methylcyclohexylamine + NAD+ + H2O
-
 ?
3-methylcyclohexanone + NH3 + NADH + H+
-
 Cystobacter fuscus 3-methylcyclohexylamine + NAD+ + H2O
-
 ?
3-methylcyclohexanone + NH3 + NADH + H+
-
 Microbacterium sp. MA1 3-methylcyclohexylamine + NAD+ + H2O
-
 ?
3-methylcyclopentanone + NH3 + NADH + H+
-
 Mycolicibacterium smegmatis 3-methylcyclopentylamine + NAD+ + H2O
-
 ?
3-methylcyclopentanone + NH3 + NADH + H+
-
 Cystobacter fuscus 3-methylcyclopentylamine + NAD+ + H2O
-
 ?
3-methylcyclopentanone + NH3 + NADH + H+
-
 Microbacterium sp. MA1 3-methylcyclopentylamine + NAD+ + H2O
-
 ?
4-hydroxybutan-2-one + NH3 + NADH + H+
-
 Mycolicibacterium smegmatis 3-aminobutan-1-ol + NAD+ + H2O
-
 ?
4-hydroxybutan-2-one + NH3 + NADH + H+
-
 Cystobacter fuscus 3-aminobutan-1-ol + NAD+ + H2O
-
 ?
4-hydroxybutan-2-one + NH3 + NADH + H+
-
 Microbacterium sp. MA1 3-aminobutan-1-ol + NAD+ + H2O
-
 ?
4-methylcyclohexanone + NH3 + NADH + H+
-
 Mycolicibacterium smegmatis 4-methylcyclohexylamine + NAD+ + H2O
-
 ?
4-methylcyclohexanone + NH3 + NADH + H+
-
 Cystobacter fuscus 4-methylcyclohexylamine + NAD+ + H2O
-
 ?
4-methylcyclohexanone + NH3 + NADH + H+
-
 Microbacterium sp. MA1 4-methylcyclohexylamine + NAD+ + H2O
-
 ?
benzaldehyde + NH3 + NADH + H+
-
 Mycolicibacterium smegmatis benzylamine + NAD+ + H2O
-
 ?
benzaldehyde + NH3 + NADH + H+
-
 Cystobacter fuscus benzylamine + NAD+ + H2O
-
 ?
benzaldehyde + NH3 + NADH + H+
-
 Microbacterium sp. MA1 benzylamine + NAD+ + H2O
-
 ?
but-3-en-2-one + NH3 + NADH + H+
-
 Mycolicibacterium smegmatis but-3-en-2-amine + NAD+ + H2O
-
 ?
but-3-en-2-one + NH3 + NADH + H+
-
 Cystobacter fuscus but-3-en-2-amine + NAD+ + H2O
-
 ?
but-3-en-2-one + NH3 + NADH + H+
-
 Microbacterium sp. MA1 but-3-en-2-amine + NAD+ + H2O
-
 ?
cyclohexanone + methylamine + NADH + H+
-
 Mycolicibacterium smegmatis N-methylcyclohexylamine + NAD+ + H2O
-
 ?
cyclohexanone + methylamine + NADH + H+
-
 Cystobacter fuscus N-methylcyclohexylamine + NAD+ + H2O
-
 ?
cyclohexanone + methylamine + NADH + H+
-
 Microbacterium sp. MA1 N-methylcyclohexylamine + NAD+ + H2O
-
 ?
cyclohexanone + NH3 + NADH + H+
-
 Mycolicibacterium smegmatis cyclohexylamine + NAD+ + H2O
-
 ?
cyclohexanone + NH3 + NADH + H+
-
 Cystobacter fuscus cyclohexylamine + NAD+ + H2O
-
 ?
cyclohexanone + NH3 + NADH + H+
-
 Microbacterium sp. MA1 cyclohexylamine + NAD+ + H2O
-
 ?
cyclopentanone + NH3 + NADPH + H+
-
 Mycolicibacterium smegmatis cyclopentylamine + NADP+ + H2O
-
 ?
cyclopentanone + NH3 + NADPH + H+
-
 Cystobacter fuscus cyclopentylamine + NADP+ + H2O
-
 ?
cyclopentanone + NH3 + NADPH + H+
-
 Microbacterium sp. MA1 cyclopentylamine + NADP+ + H2O
-
 ?
hexan-3-one + NH3 + NADH + H+
-
 Mycolicibacterium smegmatis hexan-3-amine + NAD+ + H2O
-
 ?
hexan-3-one + NH3 + NADH + H+
-
 Cystobacter fuscus hexan-3-amine + NAD+ + H2O
-
 ?
hexan-3-one + NH3 + NADH + H+
-
 Microbacterium sp. MA1 hexan-3-amine + NAD+ + H2O
-
 ?
isobutanal + methylamine + NADH + H+
-
 Mycolicibacterium smegmatis N,2-dimethylpropan-1-amine + NAD+ + H2O
-
 ?
isobutanal + methylamine + NADH + H+
-
 Cystobacter fuscus N,2-dimethylpropan-1-amine + NAD+ + H2O
-
 ?
isobutanal + methylamine + NADH + H+
-
 Microbacterium sp. MA1 N,2-dimethylpropan-1-amine + NAD+ + H2O
-
 ?
isobutanal + NH3 + NADH + H+
-
 Mycolicibacterium smegmatis 2-methylpropan-1-amine + NAD+ + H2O
-
 ?
isobutanal + NH3 + NADH + H+
-
 Cystobacter fuscus 2-methylpropan-1-amine + NAD+ + H2O
-
 ?
isobutanal + NH3 + NADH + H+
-
 Microbacterium sp. MA1 2-methylpropan-1-amine + NAD+ + H2O
-
 ?
pentan-2-one + NH3 + NADH + H+
-
 Mycolicibacterium smegmatis pentan-2-amine + NAD+ + H2O
-
 ?
pentan-2-one + NH3 + NADH + H+
-
 Cystobacter fuscus pentan-2-amine + NAD+ + H2O
-
 ?
pentan-2-one + NH3 + NADH + H+
-
 Microbacterium sp. MA1 pentan-2-amine + NAD+ + H2O
-
 ?
pentan-2-one + NH3 + NADH + H+ no substrate of wild-type, but substrate of mutants carrying substitution R161M Petrotoga mobilis pentan-2-amine + NAD+ + H2O
-
 ?
pentan-2-one + NH3 + NADH + H+ no substrate of wild-type, but substrate of mutants carrying substitution R161M Petrotoga mobilis DSM 10674 pentan-2-amine + NAD+ + H2O
-
 ?
pentan-2-one + NH3 + NADH + H+
-
 Cystobacter fuscus DSM 2262 pentan-2-amine + NAD+ + H2O
-
 ?
pentan-3-one + NH3 + NADH + H+
-
 Mycolicibacterium smegmatis pentan-3-amine + NAD+ + H2O
-
 ?
pentan-3-one + NH3 + NADH + H+
-
 Cystobacter fuscus pentan-3-amine + NAD+ + H2O
-
 ?
pentan-3-one + NH3 + NADH + H+
-
 Microbacterium sp. MA1 pentan-3-amine + NAD+ + H2O
-
 ?
pentanal + NH3 + NADH + H+
-
 Mycolicibacterium smegmatis n-pentylamine + NAD+ + H2O
-
 ?
pentanal + NH3 + NADH + H+
-
 Cystobacter fuscus n-pentylamine + NAD+ + H2O
-
 ?
pentanal + NH3 + NADH + H+
-
 Microbacterium sp. MA1 n-pentylamine + NAD+ + H2O
-
 ?

Synonyms

Synonyms Comment Organism
AmDH4
-
 Petrotoga mobilis
D187_009359
-
 Cystobacter fuscus
D187_009359
-
 Microbacterium sp. MA1
DapB_1
-
 Mycolicibacterium smegmatis
Pmob_1166
-
 Petrotoga mobilis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.04
-
 NH3 pH 9.5, 23°C Cystobacter fuscus
0.05
-
 NADPH pH 9.5, 23°C Cystobacter fuscus
0.1
-
 NADH pH 9.5, 23°C Cystobacter fuscus
0.11
-
 Isobutanal pH 9.5, 23°C Cystobacter fuscus
0.13
-
 cyclohexanone pH 9.5, 23°C Cystobacter fuscus
0.28
-
 NADPH pH 9.5, 23°C Mycolicibacterium smegmatis
0.3
-
 NH3 pH 9.5, 23°C Mycolicibacterium smegmatis
0.54
-
 Isobutanal pH 9.5, 23°C Mycolicibacterium smegmatis
0.58
-
 NADH pH 9.5, 23°C Mycolicibacterium smegmatis
0.61
-
 2-methylcyclohexanone pH 9.5, 23°C Mycolicibacterium smegmatis
0.94
-
 cyclohexanone pH 9.5, 23°C Mycolicibacterium smegmatis
2.5
-
 (2R)-2-amino-4-oxopentanoate mutant E102A, pH 9.5, 23°C Petrotoga mobilis
37.8
-
 NH3 wild-type, pH 9.5, 23°C Petrotoga mobilis

Cofactor

Cofactor Comment Organism Structure
NADH
-
 Petrotoga mobilis
NADH specific activities for both cofactors NADH and NADPH are substrate-dependent Mycolicibacterium smegmatis
NADH specific activities for both cofactors NADH and NADPH are substrate-dependent Cystobacter fuscus
NADH specific activities for both cofactors NADH and NADPH are substrate-dependent Microbacterium sp. MA1
NADPH specific activities for both cofactors NADH and NADPH are substrate-dependent Mycolicibacterium smegmatis
NADPH specific activities for both cofactors NADH and NADPH are substrate-dependent Cystobacter fuscus
NADPH specific activities for both cofactors NADH and NADPH are substrate-dependent Microbacterium sp. MA1

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.41
-
 cyclohexanone pH 9.5, 23°C Mycolicibacterium smegmatis
0.41
-
 cyclohexanone pH 9.5, 23°C Microbacterium sp. MA1
0.41
-
 cyclohexanone pH 9.5, 23°C Petrotoga mobilis
1.99
-
 cyclohexanone pH 9.5, 23°C Cystobacter fuscus
2.76
-
 Isobutanal pH 9.5, 23°C Mycolicibacterium smegmatis
2.76
-
 Isobutanal pH 9.5, 23°C Microbacterium sp. MA1
2.76
-
 Isobutanal pH 9.5, 23°C Petrotoga mobilis
4.94
-
 2-methylcyclohexanone pH 9.5, 23°C Mycolicibacterium smegmatis
4.94
-
 2-methylcyclohexanone pH 9.5, 23°C Microbacterium sp. MA1
4.94
-
 2-methylcyclohexanone pH 9.5, 23°C Petrotoga mobilis
10623
-
 NH3 pH 9.5, 23°C Cystobacter fuscus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0004
-
 NH3 pH 9.5, 23°C Cystobacter fuscus
0.0008
-
 NH3 pH 9.5, 23°C Mycolicibacterium smegmatis
0.054
-
 cyclohexanone pH 9.5, 23°C Cystobacter fuscus
0.118
-
 NH3 wild-type, pH 9.5, 23°C Petrotoga mobilis
0.209
-
 Isobutanal pH 9.5, 23°C Cystobacter fuscus
0.26
-
 2-methylcyclohexanone pH 9.5, 23°C Mycolicibacterium smegmatis
0.62
-
 Isobutanal pH 9.5, 23°C Mycolicibacterium smegmatis
1.705
-
 NADH pH 9.5, 23°C Cystobacter fuscus
1.72
-
 cyclohexanone pH 9.5, 23°C Mycolicibacterium smegmatis
1.99
-
 NADH pH 9.5, 23°C Mycolicibacterium smegmatis
2.81
-
 (2R)-2-amino-4-oxopentanoate mutant E102A, pH 9.5, 23°C Petrotoga mobilis
4.537
-
 NADPH pH 9.5, 23°C Cystobacter fuscus
4.89
-
 NADPH pH 9.5, 23°C Mycolicibacterium smegmatis
130
-
 (2R)-2-amino-4-oxopentanoate wild-type, pH 9.5, 23°C Petrotoga mobilis
1440
-
 NADH wild-type, pH 9.5, 23°C Petrotoga mobilis