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Literature summary for 1.4.1.28 extracted from

  • Mayol, O.; David, S.; Darii, E.; Debard, A.; Mariage, A.; Pellouin, V.; Petit, J.-L.; Salanoubat, M.; de Berardinis, V.; Zaparucha, A.; Vergne-Vaxelaire, C.
    Asymmetric reductive amination by a wild-type amine dehydrogenase from the thermophilic bacteria Petrotoga mobilis (2016), Catal. Sci. Technol., 6, 7421-7428 .
No PubMed abstract available
Search for more literature for 1.4.1.28

Organism

Organism UniProt Comment Textmining
Petrotoga mobilis
-
-
-
Petrotoga mobilis DSM 10674
-
-
-

Purification (Commentary)

Purification (Comment) Organism
purification by heat treatment without notable loss of material and activity Petrotoga mobilis
purified by heat treatment without notable loss of material and activity Petrotoga mobilis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.6
-
 substrate 5-oxohexanoate, pH 7.2, 25°C Petrotoga mobilis
51.9
-
 substrate 4-oxopentanoate, pH 7.2, 25°C Petrotoga mobilis

Storage Stability

Storage Stability Organism
30°C, stable for several days Petrotoga mobilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-oxopentanoate + NH3 + NADH + H+
-
 Petrotoga mobilis (4S)-4-aminopentanoate + NAD+ + H2O
-
 ?
4-oxopentanoate + NH3 + NADH + H+
-
 Petrotoga mobilis DSM 10674 (4S)-4-aminopentanoate + NAD+ + H2O
-
 ?
4-oxopentanoate + NH3 + NADPH + H+
-
 Petrotoga mobilis (4S)-4-aminopentanoate + H2O + NADP+
-
 ?
4-oxopentanoate + NH3 + NADPH + H+
-
 Petrotoga mobilis DSM 10674 (4S)-4-aminopentanoate + H2O + NADP+
-
 ?
5-oxohexanoate + NH3 + NADH + H+
-
 Petrotoga mobilis (5S)-5-aminohexanoate + NAD+ + H2O
-
 ?
5-oxohexanoate + NH3 + NADH + H+
-
 Petrotoga mobilis DSM 10674 (5S)-5-aminohexanoate + NAD+ + H2O
-
 ?
5-oxohexanoate + NH3 + NADH + H+
-
 Petrotoga mobilis (5S)-5-aminohexanoate + H2O + NAD+
-
 ?
5-oxohexanoate + NH3 + NADH + H+
-
 Petrotoga mobilis DSM 10674 (5S)-5-aminohexanoate + H2O + NAD+
-
 ?
additional information no substrates: acetophenone, cyclohexanone, penta-2-one or 2-aminopentan-2-one Petrotoga mobilis ?
-
-
additional information no substrates: acetophenone, cyclohexanone, penta-2-one or 2-aminopentan-2-one Petrotoga mobilis DSM 10674 ?
-
-

Synonyms

Synonyms Comment Organism
AmDH4
-
 Petrotoga mobilis
amine dehydrogenase
-
 Petrotoga mobilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
90
-
 activity of 0.7 U per milligram of protein Petrotoga mobilis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20
-
 hardly active at 20°C Petrotoga mobilis
60
-
 good activity Petrotoga mobilis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 half-life 65 h Petrotoga mobilis
80
-
 half-life 30 min Petrotoga mobilis

Cofactor

Cofactor Comment Organism Structure
NADH activity with NADH is 10 times higher than that with NADPH Petrotoga mobilis
NADPH activity with NADH is 10 times higher than that with NADPH Petrotoga mobilis