KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.83 | - |
lipoic acid | pH 6.3, 25°C | Synechocystis sp. PCC 6803 |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Pisum sativum | 5739 | - |
mitochondrion | - |
Arabidopsis thaliana | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
75000 | 80000 | H-protein, gel filtration | Synechocystis sp. PCC 6803 |
150000 | - |
L-protein, gel filtration | Synechocystis sp. PCC 6803 |
200000 | - |
P-protein, gel filtration | Synechocystis sp. PCC 6803 |
1550000 | 1650000 | minimum mass of the leaf GDC, mass spectrometry | Pisum sativum |
1550000 | 1650000 | minimum mass of the leaf GDC, mass spectrometry | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9M5K3 and Q94B78 and P25855 and Q9LQL0 and O65396 | Q9M5K3 i.e. dihydrolipoyl dehydrogenase component L-protein, cf. EC 1.8.1.4, Q94B78 i.e. glycine dehydrogenase component P-protein, cf. EC 1.4.4.2, P25855 i.e. component H-protein isoform H1, Q9LQL0 i.e. component H-protein isoform H3, O65396 i.e. aminomethyltransferase component T-protein, cf. EC 2.1.2.10 | - |
Pisum sativum | P31023 and P26969 and P16048 and P49364 | P31023 i.e. dihydrolipoyl dehydrogenase component L-protein, cf. EC 1.8.1.4, P26969 i.e. glycine dehydrogenase component P-protein, cf. EC 1.4.4.2, P16048 i.e. component H-protein, P49364 i.e. aminomethyltransferase component T-protein, cf. EC 2.1.2.10 | - |
Synechocystis sp. PCC 6803 | P72740 and P54261 | P72740 i.e. dihydrolipoyl dehydrogenase component L-protein, cf. EC 1.8.1.4, P54261 i.e. aminomethyltransferase component T-protein, cf. EC 2.1.2.10 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Pisum sativum | - |
leaf | - |
Arabidopsis thaliana | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
specific maximum activities are 0.37 micromol per min and mg for the P-protein (bicarbonate-exchange reaction) | Synechocystis sp. PCC 6803 |
14 | - |
assay of L-protein with lipoic acid, 25°C, pH 6.3 | Synechocystis sp. PCC 6803 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycine + tetrahydrofolate + NAD+ | - |
Synechocystis sp. PCC 6803 | 5,10-methylenetetrahydrofolate + NH3 + CO2 + NADH | - |
? | |
lipoic acid + NADH + H+ | assay of isolated L-protein, cf. EC 1.8.1.4 | Synechocystis sp. PCC 6803 | dihydrolipoic acid + NAD+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the H-isoproteins GCS-H1 and GCS-H3 are fully redundant. Isoform GCS-H2 is not present in leaf mitochondria | Arabidopsis thaliana |
More | the molar ratios of the leaf GDC component proteins are 1* L-protein dimer, 0.5 * P-protein dimer, 0.6 * T-protein and 2.4 * H-protein, determined by mass spectrometry | Synechocystis sp. PCC 6803 |
multimer | 2 * 100000, P-protein dimer, SDS-PAGE, 2 * 50832, L-protein dimer | Synechocystis sp. PCC 6803 |
multimer | the molar ratios of the leaf GDC component proteins are 1* L-protein dimer, 4 * P-protein dimer, 8 * T-protein and 20 * H-protein, determined by mass spectrometry | Arabidopsis thaliana |
multimer | the molar ratios of the leaf GDC component proteins are 1* L-protein dimer, 4 * P-protein dimer, 8 * T-protein and 26 H-protein, determined by mass spectrometry | Pisum sativum |
Synonyms | Comment | Organism |
---|---|---|
At1g11860 | gene name, aminomethyltransferase | Arabidopsis thaliana |
At1g32470 | gene name, H-protein isoform GCS-H1 | Arabidopsis thaliana |
At1g48030 | gene name, dihydrolipoyl dehydrogenase | Arabidopsis thaliana |
AT2G35370 | gene name, H-protein isoform GCS-H1 | Arabidopsis thaliana |
At4g33010 | gene name, glycine dehydrogenase | Arabidopsis thaliana |
GDCSH | H-protein | Pisum sativum |
GDCSP | glycine dehydrogenase | Pisum sativum |
GDSCT | aminomethyltransferase | Pisum sativum |
sll0171 | gene name, aminomethyltransferase | Synechocystis sp. PCC 6803 |
slr1096 | gene name, L-protein | Synechocystis sp. PCC 6803 |