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Literature summary for 1.4.1.26 extracted from

  • Fukuyama, S.; Mihara, H.; Miyake, R.; Ueda, M.; Esaki, N.; Kurihara, T.
    Characterization of a thermostable 2,4-diaminopentanoate dehydrogenase from Fervidobacterium nodosum Rt17-B1 (2014), J. Biosci. Bioeng., 117, 551-556 .
    View publication on PubMed
Search for more literature for 1.4.1.26

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta (DE3) cells Fervidobacterium nodosum

Inhibitors

Inhibitors Comment Organism Structure
(2R, 4S)-2,4-diaminopentanoate uncompetitive substrate inhibition Fervidobacterium nodosum
Ca2+ 85% residual activity at 0.5 mM Fervidobacterium nodosum
Co2+ 34% residual activity at 0.5 mM Fervidobacterium nodosum
Cu2+ complete inhibition at 0.5 mM Fervidobacterium nodosum
D-alanine enzymatic activity decreases to 71% of the positive control at 5 mM Fervidobacterium nodosum
D-Lysine enzymatic activity decreases to 68% of the positive control at 5 mM Fervidobacterium nodosum
D-ornithine uncompetitive inhibition, enzymatic activity decreases to 14% of the positive control at 5 mM Fervidobacterium nodosum
Fe2+ 84% residual activity at 0.5 mM Fervidobacterium nodosum
Mg2+ 65% residual activity at 0.5 mM Fervidobacterium nodosum
additional information EDTA does not affect the enzyme activity Fervidobacterium nodosum
Ni2+ 2% residual activity at 0.5 mM Fervidobacterium nodosum
Zn2+ 2% residual activity at 0.5 mM Fervidobacterium nodosum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.05
-
 NAD+ at pH 8.5 and 55°C Fervidobacterium nodosum
0.2
-
 (2R,4S)-2,4-diaminopentanoate with NAD+ as cosubstrate, at pH 8.5 and 55°C Fervidobacterium nodosum
2
-
 NADP+ at pH 8.5 and 55°C Fervidobacterium nodosum
2
-
 2,5-Diaminohexanoate with NAD+ as cosubstrate, at pH 8.5 and 55°C Fervidobacterium nodosum

Metals/Ions

Metals/Ions Comment Organism Structure
additional information K+ and Mn2+ have no significant effect on the enzyme activity Fervidobacterium nodosum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(2R,4S)-2,4-diaminopentanoate + H2O + NAD+ Fervidobacterium nodosum
-
 (2R)-2-amino-4-oxopentanoate + NH3 + NADH + H+
-
 ?
(2R,4S)-2,4-diaminopentanoate + H2O + NADP+ Fervidobacterium nodosum
-
 (2R)-2-amino-4-oxopentanoate + NH3 + NADPH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Fervidobacterium nodosum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
His-Bind column chromatography Fervidobacterium nodosum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1
-
 with 2,5-diaminohexanoate as substrate, at pH 8.5 and 55°C Fervidobacterium nodosum
92
-
 with (2R,4S)-2,4-diaminopentanoate as substrate, at pH 8.5 and 55°C Fervidobacterium nodosum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2R,4S)-2,4-diaminopentanoate + H2O + NAD+
-
 Fervidobacterium nodosum (2R)-2-amino-4-oxopentanoate + NH3 + NADH + H+
-
 ?
(2R,4S)-2,4-diaminopentanoate + H2O + NADP+
-
 Fervidobacterium nodosum (2R)-2-amino-4-oxopentanoate + NH3 + NADPH + H+
-
 ?
2,5-diaminohexanoate + H2O + NAD+ 90fold less activity with 2,5-diaminohexanoate compared to (2R,4S)-2,4-diaminopentanoate Fervidobacterium nodosum 2-amino-5-oxohexanoate + NH3 + NADH + H+
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 38000, SDS-PAGE Fervidobacterium nodosum
homodimer 2 * 38000, calculated from amino acid sequence Fervidobacterium nodosum

Synonyms

Synonyms Comment Organism
2,4-DAPDH
-
 Fervidobacterium nodosum
2,4-diaminopentanoate dehydrogenase
-
 Fervidobacterium nodosum
Fnod_1646
-
 Fervidobacterium nodosum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
85
-
-
 Fervidobacterium nodosum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
70 100 more than 50% activity between 70 and 100°C Fervidobacterium nodosum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
90 100 the half-denaturation time at 90°C and 100°C is 38 min and 2 min, respectively Fervidobacterium nodosum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0006
-
 2,5-Diaminohexanoate with NAD+ as cosubstrate, at pH 8.5 and 55°C Fervidobacterium nodosum
57
-
 NADP+ at pH 8.5 and 55°C Fervidobacterium nodosum
65
-
 NAD+ at pH 8.5 and 55°C Fervidobacterium nodosum
65
-
 (2R,4S)-2,4-diaminopentanoate with NAD+ as cosubstrate, at pH 8.5 and 55°C Fervidobacterium nodosum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5
-
-
 Fervidobacterium nodosum

pH Range

pH Minimum pH Maximum Comment Organism
9 10 the enzyme shows more than 70% of maximum activity in the pH range of 9.0-10.0 Fervidobacterium nodosum

Cofactor

Cofactor Comment Organism Structure
NAD+ the Km value for NAD+ is about 40 times lower than that for NADP+ Fervidobacterium nodosum
NADP+
-
 Fervidobacterium nodosum
pyridoxal 5'-phosphate
-
 Fervidobacterium nodosum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.1
-
 D-ornithine at pH 8.5 and 55°C Fervidobacterium nodosum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0003
-
 2,5-Diaminohexanoate with NAD+ as cosubstrate, at pH 8.5 and 55°C Fervidobacterium nodosum
29
-
 NADP+ at pH 8.5 and 55°C Fervidobacterium nodosum
330
-
 (2R,4S)-2,4-diaminopentanoate with NAD+ as cosubstrate, at pH 8.5 and 55°C Fervidobacterium nodosum
1300
-
 NAD+ at pH 8.5 and 55°C Fervidobacterium nodosum