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Literature summary for 1.4.1.23 extracted from

  • Navarrete, R.M.; Vara, J.A.; Hutchinson, C.R.
    Purification of an inducible L-valine dehydrogenase of Streptomyces coelicolor A3(2) (1990), J. Gen. Microbiol., 136, 273-281.
    View publication on PubMed
Search for more literature for 1.4.1.23

Inhibitors

Inhibitors Comment Organism Structure
L-valine substrate inhibition, noncompetitive Streptomyces coelicolor

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41000
-
 2 * 41000, SDS-PAGE Streptomyces coelicolor
70000
-
 gel filtration Streptomyces coelicolor

Organism

Organism UniProt Comment Textmining
Streptomyces coelicolor
-
-
-
Streptomyces coelicolor A3(2)
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Streptomyces coelicolor

Reaction

Reaction Comment Organism Reaction ID
L-valine + H2O + NAD+ = 3-methyl-2-oxobutanoate + NH3 + NADH + H+ sequential ordered mechanism with non-competitive inhibition by valine Streptomyces coelicolor
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
14
-
 pH 10.4, 30°C, substrate L-valine Streptomyces coelicolor
19
-
 pH 10.4, 30°C, substrate 2-aminobutanoate Streptomyces coelicolor

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-2-aminobutanoate + H2O + NAD+ 130% of the activity with L-valine Streptomyces coelicolor 2-oxobutanoate + NH3 + NADH + H+
-
 r
L-alanine + H2O + NAD+ 3% of the activity with L-valine Streptomyces coelicolor pyruvate + NH3 + NADH + H+
-
 r
L-isoleucine + H2O + NAD+ 28% of the activity with L-valine Streptomyces coelicolor 3-methyl-2-oxopentanoate + NH3 + NADH + H+
-
 r
L-leucine + H2O + NAD+ 8% of the activity with L-valine Streptomyces coelicolor 2-oxoisohexanoate + NH3 + NADH + H+
-
 r
L-valine + H2O + NAD+
-
 Streptomyces coelicolor 3-methyl-2-oxobutanoate + NH3 + NADH + H+
-
 r

Subunits

Subunits Comment Organism
dimer 2 * 41000, SDS-PAGE Streptomyces coelicolor

Synonyms

Synonyms Comment Organism
VDH
-
 Streptomyces coelicolor

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
 reductive amination Streptomyces coelicolor
10.5
-
 oxidative deamination Streptomyces coelicolor

Cofactor

Cofactor Comment Organism Structure
additional information no cofactor: NADP+ Streptomyces coelicolor
NAD+
-
 Streptomyces coelicolor
NADH
-
 Streptomyces coelicolor

Expression

Organism Comment Expression
Streptomyces coelicolor activity is repressed in the presence of glucose and NH4+ or glycerol and NH4+ or asparagine down
Streptomyces coelicolor activity is induced in presence of D- and L-valine up