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Literature summary for 1.4.1.21 extracted from

  • Yoneda, K.; Kawakami, R.; Tagashira, Y.; Sakuraba, H.; Goda, S.; Ohshima, T.
    The first archaeal L-aspartate dehydrogenase from the hyperthermophile Archaeoglobus fulgidus: Gene cloning and enzymological characterization (2006), Biochim. Biophys. Acta, 1764, 1087-1093.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information unaffected by EDTA, CaCl2, NiCl2, CoCl2, CuSO4 or ZnCl2 Archaeoglobus fulgidus

Application

Application Comment Organism
additional information first report of an archaeal L-aspartate dehydrogenase, within the archaeal domain, homologues in many methanogenic species, but not in Thermococcales or Sulfolobales species Archaeoglobus fulgidus

Cloned(Commentary)

Cloned (Comment) Organism
ligated into the expression vector pET11a, expression in Escherichia coli strain BL21(DE3) Archaeoglobus fulgidus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.014
-
NADH
-
Archaeoglobus fulgidus
0.11
-
NAD+
-
Archaeoglobus fulgidus
0.19
-
L-aspartate with NAD+ as the electron acceptor Archaeoglobus fulgidus
0.32
-
NADP+
-
Archaeoglobus fulgidus
1.2
-
oxaloacetate
-
Archaeoglobus fulgidus
4.3
-
L-aspartate with NADP+ as the electron acceptor Archaeoglobus fulgidus
167
-
NH4+
-
Archaeoglobus fulgidus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26000
-
2 * 26000, SDS-PAGE, 2 * 26208, sequence analysis Archaeoglobus fulgidus
26208
-
2 * 26000, SDS-PAGE, 2 * 26208, sequence analysis Archaeoglobus fulgidus
48000
-
gel filtration Archaeoglobus fulgidus

Organism

Organism UniProt Comment Textmining
Archaeoglobus fulgidus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity by heat treatment and affinity chromatography Archaeoglobus fulgidus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.6
-
purified enzyme, at 50 °C Archaeoglobus fulgidus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-aspartate + NAD(P)+ + H2O the enzyme shows pro-R (A-type) stereospecificity for hydrogen transfer from the C4 position of the nicotinamide moiety ofNADH Archaeoglobus fulgidus oxaloacetate + NH4+ + NAD(P)H
-
?
L-aspartate + NAD+
-
Archaeoglobus fulgidus oxaloacetate + NH4+ + NADH
-
?
additional information no activity with D-aspartate, L-glutamate, L-alanine, L-leucine, L-phenylalanine, L-proline, glycine, L-serine, L-lysine, L-norvaline, L-norleucine, L-homoserine and L-2-amino-n-butyrate Archaeoglobus fulgidus ?
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 26000, SDS-PAGE, 2 * 26208, sequence analysis Archaeoglobus fulgidus

Synonyms

Synonyms Comment Organism
L-aspartate dehydrogenase
-
Archaeoglobus fulgidus
L-aspDH
-
Archaeoglobus fulgidus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
-
Archaeoglobus fulgidus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
37 100
-
Archaeoglobus fulgidus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
stable for 1 h Archaeoglobus fulgidus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
11.6
-
-
Archaeoglobus fulgidus

Cofactor

Cofactor Comment Organism Structure
NAD(P)+
-
Archaeoglobus fulgidus