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Literature summary for 1.4.1.20 extracted from

  • Yousefi, F.; Ataei, F.; Arab, S.S.; Hosseinkhani, S.
    Increase of Bacillus badius phenylalanine dehydrogenase specificity towards phenylalanine substrate by site-directed mutagenesis (2017), Arch. Biochem. Biophys., 635, 44-51 .
    View publication on PubMed
Search for more literature for 1.4.1.20

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Bacillus badius

Crystallization (Commentary)

Crystallization (Comment) Organism
molecular modeling of structure and docking of substrates. In mutant V144L, the distances of active/binding site and substrate with an appropriate orientation decrease for phenylalanine and increase for tyrosine. In mutant V144N, the distances of active/binding site and substrate are increased for phenylalanine and are reduced for tyrosine. In mutant V144D, distances considerably increase with an inappropriate orientation Bacillus badius

Protein Variants

Protein Variants Comment Organism
V144D mutantion significantly reduces kcat value and also decreases Km value for phenylalanine relative to that of wild-type Bacillus badius
V144L mutation considerably increases specific activity toward phenylalanine and decreases toward L-tyrosine. ThePhe/Tyr specificity constant in V144L increases more than 4fold Bacillus badius
V144N mutation reduces the specific activity toward phenylalanine and increases toward tyrosine Bacillus badius

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
 L-phenylalanine mutant V144L, pH 10.4, 25°C Bacillus badius
0.06
-
 L-phenylalanine mutant V144D, pH 10.4, 25°C Bacillus badius
0.08
-
 L-phenylalanine wild-type, pH 10.4, 25°C Bacillus badius
0.19
-
 L-phenylalanine mutant V144N, pH 10.4, 25°C Bacillus badius
4.7
-
 L-tyrosine mutant V144N, pH 10.4, 25°C Bacillus badius
8.6
-
 L-tyrosine wild-type, pH 10.4, 25°C Bacillus badius
11.9
-
 L-tyrosine mutant V144L, pH 10.4, 25°C Bacillus badius
27
-
 L-tyrosine mutant V144D, pH 10.4, 25°C Bacillus badius

Organism

Organism UniProt Comment Textmining
Bacillus badius Q59224
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-phenylalanine + H2O + NAD+
-
 Bacillus badius phenylpyruvate + NH3 + NADH + H+
-
 ?
L-tyrosine + H2O + NAD+
-
 Bacillus badius (4-hydroxyphenyl)pyruvate + NH3 + NADH + H+
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3 6 L-phenylalanine mutant V144L, pH 10.4, 25°C Bacillus badius
13.5
-
 L-phenylalanine mutant V144D, pH 10.4, 25°C Bacillus badius
23
-
 L-phenylalanine mutant V144N, pH 10.4, 25°C Bacillus badius
26
-
 L-tyrosine mutant V144L, pH 10.4, 25°C Bacillus badius
28.5
-
 L-tyrosine mutant V144D, pH 10.4, 25°C Bacillus badius
50
-
 L-phenylalanine wild-type, pH 10.4, 25°C Bacillus badius
57
-
 L-tyrosine mutant V144N, pH 10.4, 25°C Bacillus badius
58
-
 L-tyrosine wild-type, pH 10.4, 25°C Bacillus badius

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1
-
 L-tyrosine mutant V144D, pH 10.4, 25°C Bacillus badius
2.2
-
 L-tyrosine mutant V144L, pH 10.4, 25°C Bacillus badius
6.7
-
 L-tyrosine wild-type, pH 10.4, 25°C Bacillus badius
11.9
-
 L-tyrosine mutant V144N, pH 10.4, 25°C Bacillus badius
118
-
 L-phenylalanine mutant V144N, pH 10.4, 25°C Bacillus badius
204
-
 L-phenylalanine mutant V144D, pH 10.4, 25°C Bacillus badius
609
-
 L-phenylalanine wild-type, pH 10.4, 25°C Bacillus badius
800
-
 L-phenylalanine mutant V144L, pH 10.4, 25°C Bacillus badius