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Literature summary for 1.4.1.20 extracted from

  • Omidinia, E.; Shahbaz Mohamadi, H.; Dinarvand, R.; Taherkhani, H.A.
    Investigation of chromatography and polymer/salt aqueous two-phase processes for downstream processing development of recombinant phenylalanine dehydrogenase (2010), Bioprocess Biosyst. Eng., 33, 317-329.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
espressed as recombinant protein in Escherichia coli strain BL21 (DE3) Bacillus badius

Organism

Organism UniProt Comment Textmining
Bacillus badius
-
-
-

Purification (Commentary)

Purification (Comment) Organism
best purification results of recombinant PheDH are achieved in an polymer/salt aqueous two-phase systems (ATPS) with 9% of phenazine ethosulfate with a molecular mass of 6000 Da (PEG-6000) and 16% of K2HPO4 Bacillus badius

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
553.6
-
after gel filtration protocol Bacillus badius
4231
-
after polymer/salt aqueous two-phase systems (ATPS) protocol Bacillus badius

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-phenylalanine + H2O + NAD+
-
Bacillus badius phenylpyruvate + NH3 + NADH + H+
-
?

Synonyms

Synonyms Comment Organism
PheDH
-
Bacillus badius
phenylalanine dehydrogenase
-
Bacillus badius
recombinant PheDH
-
Bacillus badius
recombinant phenylalanine dehydrogenase
-
Bacillus badius