Literature summary for 1.4.1.20 extracted from

Villalonga, R.; Fujii, A.; Shinohara, H.; Tachibana, S.; Asano, Y.
Covalent immobilization of phenylalanine dehydrogenase on cellulose membrane for biosensor construction (2008), Sens. Actuators B Chem., B129, 195-199.

No PubMed abstract available

Search for more literature for 1.4.1.20

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3)/pBBNH cells	Bacillus badius

General Stability

General Stability	Organism
PheDH covalently immobilized on amino-activated cellulose membrane by cross-linking with glutaraldehyde retains 82% of the initial activity, the enzyme-coated electrode retains full electrocatalytic activity after 16 days of storage at 4°C in 50 mM sodium phosphate buffer (pH 7.0)	Bacillus badius

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.21	-	L-Phe	native enzyme, at 25°C, in 100 mM glycine/KCl/KOH buffer pH 10.4	Bacillus badius
24	-	L-Phe	immobilized enzyme, at 25°C, in 100 mM glycine/KCl/KOH buffer pH 10.4	Bacillus badius

Organism

Organism	UniProt	Comment	Textmining
Bacillus badius	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-affinity column chromatography and anion exchange column chromatography	Bacillus badius

Storage Stability

Storage Stability	Organism
4°C, immobilized on amino-activated cellulose membrane in 50 mM sodium phosphate buffer (pH 7.0), 16 days, no loss of activity	Bacillus badius

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-Phe + H2O + NAD+	-	Bacillus badius	phenylpyruvate + NH3 + NADH	-	?

Synonyms

Synonyms	Comment	Organism
PheDH	-	Bacillus badius

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
40	-	native enzyme	Bacillus badius
60	-	immobilized enzyme	Bacillus badius

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
10.4	-	immobilized enzyme	Bacillus badius
11	12	native enzyme	Bacillus badius

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	-	Bacillus badius

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Release 2023.1 (January 2023)