Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 (DE3)/pBBNH cells | Bacillus badius |
General Stability | Organism |
---|---|
2.5 mM L-Phe after 21 days of incubation at 4°C in 50 mM sodium phosphate buffer, pH 7.0 | Bacillus badius |
the enzyme-coated Au-CD electrode is highly stable, retaining about 97% of its initial electrocatalytic response toward | Bacillus badius |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus badius | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-affinity column chromatography | Bacillus badius |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
the enzyme immobilized on Au-CD electrodes has a specific activity of 10.3 U/mg representing about 56% and 73% of the initial catalytic activity of native and adamantane-modified PheDH, respectively | Bacillus badius |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Phe + H2O + NAD+ | - |
Bacillus badius | phenylpyruvate + NH3 + NADH | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PheDH | - |
Bacillus badius |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Bacillus badius |