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Literature summary for 1.4.1.20 extracted from

  • Misono, H.; Yonezawa, J.; Nagata, S.; Nagasaki, S.
    Purification and characterization of a dimeric phenylalanine dehydrogenase from Rhodococcus maris K-18 (1989), J. Bacteriol., 171, 30-36.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.4.1.20

General Stability

General Stability Organism
20% glycerol, 0.25 M sodium malonate or 0.5 M sodium glutarate stabilize the enzyme during storage at 4°C Dietzia maris
unstable in absence of glycerol and salt Dietzia maris

Inhibitors

Inhibitors Comment Organism Structure
3-Phenylpropionate competitive with L-Phe Dietzia maris
4-hydroxyphenylethylamine competitive with L-Phe Dietzia maris
alpha-methyl-DL-phenylalanine
-
 Dietzia maris
Cu2+ slight Dietzia maris
D-ethionine
-
 Dietzia maris
D-Phe competitive with L-Phe Dietzia maris
D-Tyr competitive with L-Phe Dietzia maris
HgCl2 0.01 mM, complete inhibition Dietzia maris
L-Phe product inhibition Dietzia maris
L-phenylglycine competitive with L-Phe Dietzia maris
NAD+ product inhibition Dietzia maris
PCMB 0.001 mM, complete inhibition Dietzia maris
phenylenediamine
-
 Dietzia maris
Phenylethylamine competitive with L-Phe Dietzia maris
trans-cinnamate competitive with L-Phe Dietzia maris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.18
-
 deamino-NAD+
-
 Dietzia maris
0.18
-
 thionicotinamide-NAD+
-
 Dietzia maris
0.25
-
 NAD+
-
 Dietzia maris
0.25
-
 3-acetylpyridine-NAD+
-
 Dietzia maris
0.5
-
 phenylpyruvate
-
 Dietzia maris
1.3
-
 4-hydroxyphenylpyruvate
-
 Dietzia maris

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
 2 * 36000, SDS-PAGE Dietzia maris
70000
-
 gel filtration Dietzia maris

Organism

Organism UniProt Comment Textmining
Dietzia maris
-
 K-18
-
Dietzia maris K-18
-
 K-18
-

Purification (Commentary)

Purification (Comment) Organism
-
 Dietzia maris

Reaction

Reaction Comment Organism Reaction ID
L-phenylalanine + H2O + NAD+ = phenylpyruvate + NH3 + NADH + H+ the reductive amination proceeds through a sequential ordered ternary-binary mechanism Dietzia maris
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
65.2
-
-
 Dietzia maris

Storage Stability

Storage Stability Organism
4°C, 0.25 M sodium malonate, stable for 1 month Dietzia maris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-hydroxyphenylpyruvate + NH3 + NADH + H+
-
 Dietzia maris L-tyrosine + NAD+ + H2O
-
 r
L-alpha-amino-beta-phenylbutyrate + H2O + NAD+ 7.0% of the activity with L-Phe Dietzia maris 2-oxo-3-phenylbutyrate + NADH + NH3
-
 ?
L-ethionine + H2O + NAD+ 13.0% of the activity with L-Phe Dietzia maris 4-ethylthio-2-oxobutanoate + NH3 + NADH
-
 ?
L-norleucine + H2O + NAD+ 15.6% of the activity with L-Phe Dietzia maris 2-oxohexanoic acid + NADH + NH3
-
 ?
L-Phe + H2O + NAD+ the reductive amination proceeds through a sequential ordered ternary-binary mechanism Dietzia maris phenylpyruvate + NH3 + NADH
-
 r
L-Phe + H2O + NAD+ the reductive amination proceeds through a sequential ordered ternary-binary mechanism Dietzia maris K-18 phenylpyruvate + NH3 + NADH
-
 r
L-phenylalanine + H2O + 3-acetylpyridine-NAD+ at 241% of the activity with NAD+ Dietzia maris phenylpyruvate + NH3 + acetylpyridine-NADH
-
 ?
L-phenylalanine + H2O + 3-acetylpyridine-NAD+ at 241% of the activity with NAD+ Dietzia maris K-18 phenylpyruvate + NH3 + acetylpyridine-NADH
-
 ?
L-phenylalanine + H2O + 3-pyridinealdehyde-NAD+ at 9.2% of the activity with NAD+ Dietzia maris phenylpyruvate + NH3 + 3-pyridinealdehyde-NADH
-
 ?
L-phenylalanine + H2O + deamino-NAD+ at 86% of the activity with NAD+ Dietzia maris phenylpyruvate + NH3 + deamino-NADH
-
 ?
L-phenylalanine + H2O + deamino-NAD+ at 86% of the activity with NAD+ Dietzia maris K-18 phenylpyruvate + NH3 + deamino-NADH
-
 ?
L-phenylalanine + H2O + NADP+ at 6.6% of the activity with NAD+ Dietzia maris phenylpyruvate + NH3 + NADPH
-
 ?
L-phenylalanine + H2O + NADP+ at 6.6% of the activity with NAD+ Dietzia maris K-18 phenylpyruvate + NH3 + NADPH
-
 ?
L-phenylalanine + H2O + thionicotinamide-NAD+ at 101% of the activity with NAD+ Dietzia maris phenylpyruvate + NH3 + thionicotinamide-NADH
-
 ?
L-phenylalanine + H2O + thionicotinamide-NAD+ at 101% of the activity with NAD+ Dietzia maris K-18 phenylpyruvate + NH3 + thionicotinamide-NADH
-
 ?
L-Trp + H2O + NAD+ 7.5% of the activity with L-Phe Dietzia maris indole-3-pyruvate + NH3 + NADH
-
 ?
m-fluoro-DL-phenylalanine + H2O + NAD+ 7.7% of the activity with L-Phe Dietzia maris 3-(3-fluorophenyl)-2-oxopropionate + NADH + NH3
-
 ?
p-fluoro-DL-phenylalanine + H2O + NAD+ 8.3% of the activity with L-Phe Dietzia maris (4-fluorophenyl)-2-oxopropionate + NADH + NH3
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 36000, SDS-PAGE Dietzia maris

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.8
-
 reductive amination of phenylpyruvate Dietzia maris
10.8
-
 oxidative deamination of L-Phe Dietzia maris

Cofactor

Cofactor Comment Organism Structure
3-acetylpyridine-NAD+ 241% of the activity with NAD+ in the reaction with L-Phe Dietzia maris
3-pyridinealdehyde-NAD+ 9.2% of the activity with NAD+ in the reaction with L-Phe Dietzia maris
deamino-NAD+ 86% of the activity with NAD+ in the reaction with L-Phe Dietzia maris
NAD+
-
 Dietzia maris
NADH
-
 Dietzia maris
thionicotinamide-NAD+ 101% of the activity with NAD+ in the reaction with L-Phe Dietzia maris

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.14
-
 D-Phe
-
 Dietzia maris
1.52
-
 Phenylethylamine
-
 Dietzia maris
1.58
-
 D-Tyr
-
 Dietzia maris
1.76
-
 D-ethionine
-
 Dietzia maris
2.6
-
 3-Phenylpropionate
-
 Dietzia maris
4.36
-
 phenylenediamine
-
 Dietzia maris
8.32
-
 L-phenylglycine
-
 Dietzia maris
12.7
-
 trans-cinnamic acid
-
 Dietzia maris
13.7
-
 4-hydroxyphenylethylamine
-
 Dietzia maris
20.8
-
 alpha-methyl-DL-phenylalanine
-
 Dietzia maris