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Literature summary for 1.4.1.2 extracted from

  • Wakamatsu, T.; Higashi, C.; Ohmori, T.; Doi, K.; Ohshima, T.
    Biochemical characterization of two glutamate dehydrogenases with different cofactor specificities from a hyperthermophilic archaeon Pyrobaculum calidifontis (2013), Extremophiles, 17, 379-389 .
    View publication on PubMed
Search for more literature for 1.4.1.2

Activating Compound

Activating Compound Comment Organism Structure
isocitrate 10 M, 112% of initial activity Pyrobaculum calidifontis

Inhibitors

Inhibitors Comment Organism Structure
malate 10 mM, 75% residual activity Pyrobaculum calidifontis
oxaloacetate 10 mM, 60% residual activity Pyrobaculum calidifontis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
 NADH pH 9.5, 50°C Pyrobaculum calidifontis
0.028
-
 NAD+ pH 10.5, 50°C Pyrobaculum calidifontis
0.92
-
 2-oxoglutarate pH 9.5, 50°C Pyrobaculum calidifontis
5.3
-
 L-glutamate pH 10.5, 50°C Pyrobaculum calidifontis
9.8
-
 NH3 pH 9.5, 50°C Pyrobaculum calidifontis

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ 1 mM, 109% of initial activity Pyrobaculum calidifontis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
286000
-
 gel filtration Pyrobaculum calidifontis

Organism

Organism UniProt Comment Textmining
Pyrobaculum calidifontis A3MUY9
-
-
Pyrobaculum calidifontis JCM 11548 A3MUY9
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxobutanoate + NH3 + NADH + H+ 2.7% of the activity with 2-oxoglutarate Pyrobaculum calidifontis L-2-aminobutanoate + H2O + NAD+
-
 r
2-oxobutanoate + NH3 + NADH + H+ 2.7% of the activity with 2-oxoglutarate Pyrobaculum calidifontis JCM 11548 L-2-aminobutanoate + H2O + NAD+
-
 r
2-oxocaproate + NH3 + NADH + H+ 1.3% of the activity with 2-oxoglutarate Pyrobaculum calidifontis L-2-aminohexanoate + H2O + NAD+
-
 r
2-oxoglutarate + NH3 + NADH + H+
-
 Pyrobaculum calidifontis L-glutamate + H2O + NAD+
-
 r
2-oxoglutarate + NH3 + NADH + H+
-
 Pyrobaculum calidifontis JCM 11548 L-glutamate + H2O + NAD+
-
 r
2-oxovalerate + NH3 + NADH + H+ 6.6% of the activity with 2-oxoglutarate Pyrobaculum calidifontis L-valine + H2O + NAD+
-
 r
L-2-aminobutanoate + H2O + NAD+ 8.4% of the activity with L-glutamate Pyrobaculum calidifontis 2-oxobutanoate + NH3 + NADH + H+
-
 r
L-2-aminobutanoate + H2O + NAD+ 8.4% of the activity with L-glutamate Pyrobaculum calidifontis JCM 11548 2-oxobutanoate + NH3 + NADH + H+
-
 r
L-glutamate + H2O + NAD+
-
 Pyrobaculum calidifontis 2-oxoglutarate + NH3 + NADH + H+
-
 r
L-glutamate + H2O + NAD+
-
 Pyrobaculum calidifontis JCM 11548 2-oxoglutarate + NH3 + NADH + H+
-
 r
L-isoleucine + H2O + NAD+ 3.0% of the activity with L-glutamate Pyrobaculum calidifontis 2-oxo-3-methylvalerate + NH3 + NADH + H+
-
 r
L-leucine + H2O + NAD+ 1.6% of the activity with L-glutamate Pyrobaculum calidifontis 2-oxo-iso-valerate + NH3 + NADH + H+
-
 r
L-norvaline + H2O + NAD+ 35% of the activity with L-glutamate Pyrobaculum calidifontis 2-oxopentanoate + NH3 + NADH + H+
-
 r
L-norvaline + H2O + NAD+ 35% of the activity with L-glutamate Pyrobaculum calidifontis JCM 11548 2-oxopentanoate + NH3 + NADH + H+
-
 r
L-valine + H2O + NAD+ 31% of the activity with L-glutamate Pyrobaculum calidifontis 2-oxovalerate + NH3 + NADH + H+
-
 r

Subunits

Subunits Comment Organism
hexamer 6 * 48200, calculated Pyrobaculum calidifontis

Synonyms

Synonyms Comment Organism
Pcal_1031
-
 Pyrobaculum calidifontis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
14
-
 NAD+ pH 10.5, 50°C Pyrobaculum calidifontis
17
-
 L-glutamate pH 10.5, 50°C Pyrobaculum calidifontis
58
-
 NADH pH 9.5, 50°C Pyrobaculum calidifontis
58
-
 NH3 pH 9.5, 50°C Pyrobaculum calidifontis
65
-
 2-oxoglutarate pH 9.5, 50°C Pyrobaculum calidifontis

Cofactor

Cofactor Comment Organism Structure
additional information no cofactor: NADP+ Pyrobaculum calidifontis
NAD+
-
 Pyrobaculum calidifontis
NADH
-
 Pyrobaculum calidifontis
NADPH about 1% of th activity with NADH Pyrobaculum calidifontis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2
-
 L-glutamate pH 10.5, 50°C Pyrobaculum calidifontis
5.9
-
 NH3 pH 9.5, 50°C Pyrobaculum calidifontis
71
-
 2-oxoglutarate pH 9.5, 50°C Pyrobaculum calidifontis
500
-
 NAD+ pH 10.5, 50°C Pyrobaculum calidifontis
5800
-
 NADH pH 9.5, 50°C Pyrobaculum calidifontis