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Literature summary for 1.4.1.2 extracted from

  • Bonete, M.J.; Perez-Pomares, F.; Ferrer, J.; Camacho, M.L.
    NAD-glutamate dehydrogenase from Halobacterium halobium: inhibition and activation by TCA intermediates and amino acids (1996), Biochim. Biophys. Acta, 1289, 14-24.
    View publication on PubMed
Search for more literature for 1.4.1.2

Activating Compound

Activating Compound Comment Organism Structure
glycine
-
 Halobacterium salinarum
L-alanine
-
 Halobacterium salinarum
L-arginine
-
 Halobacterium salinarum
L-asparagine
-
 Halobacterium salinarum
L-aspartic acid
-
 Halobacterium salinarum
L-cysteine
-
 Halobacterium salinarum
L-glutamine
-
 Halobacterium salinarum
L-histidine
-
 Halobacterium salinarum
L-isoleucine
-
 Halobacterium salinarum
L-leucine
-
 Halobacterium salinarum
L-lysine
-
 Halobacterium salinarum
L-methionine
-
 Halobacterium salinarum
L-phenylalanine
-
 Halobacterium salinarum
L-serine
-
 Halobacterium salinarum
L-threonine
-
 Halobacterium salinarum
L-tryptophan
-
 Halobacterium salinarum
L-tyrosine
-
 Halobacterium salinarum
L-valine
-
 Halobacterium salinarum

Inhibitors

Inhibitors Comment Organism Structure
2-oxoglutarate non-competitive versus L-glutamate, competitive versus NAD+ Halobacterium salinarum
adipate
-
 Halobacterium salinarum
D-glutamate non-competitive versus L-glutamate, non-competitive versus NAD+ Halobacterium salinarum
fumarate non-competitive versus L-glutamate, competitive versus NAD+ Halobacterium salinarum
Glutarate uncompetitive versus L-glutamate, competitive versus NAD+ Halobacterium salinarum
malate non-competitive versus L-glutamate, non-competitive versus NAD+ Halobacterium salinarum
additional information GTP, ATP, ADP and AMP do not affect the activity Halobacterium salinarum
NADP+ non-competitive versus L-glutamate, non-competitive versus NAD+ Halobacterium salinarum
oxaloacetate non-competitive versus L-glutamate, non-competitive versus NAD+ Halobacterium salinarum
succinate non-competitive versus L-glutamate, non-competitive versus NAD+ Halobacterium salinarum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-glutamate + H2O + NAD+ Halobacterium salinarum
-
 2-oxoglutarate + NH3 + NADH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Halobacterium salinarum
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamate + H2O + NAD+
-
 Halobacterium salinarum 2-oxoglutarate + NH3 + NADH + H+
-
 ?

Synonyms

Synonyms Comment Organism
NAD-GDH
-
 Halobacterium salinarum
NAD-glutamate dehydrogenase
-
 Halobacterium salinarum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 assay at Halobacterium salinarum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Halobacterium salinarum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.78
-
 NADP+ pH 8.0, 40°C, Ki(slope), at 120 mM L-glutamate Halobacterium salinarum
1
-
 2-oxoglutarate pH 8.0, 40°C, Ki(slope), at 2.5 mM NAD+ Halobacterium salinarum
1.54
-
 fumarate pH 8.0, 40°C, Ki(slope), at 2.5 mM NAD+ Halobacterium salinarum
2.5
-
 2-oxoglutarate pH 8.0, 40°C, Ki(intercept), at 2.5 mM NAD+ Halobacterium salinarum
3
-
 D-glutamate pH 8.0, 40°C, Ki(slope), at 2.5 mM NAD+ Halobacterium salinarum
3
-
 Glutarate pH 8.0, 40°C, Ki(slope), at 2.5 mM NAD+ Halobacterium salinarum
3 7 oxaloacetate pH 8.0, 40°C, Ki(intercept), at 2.5 mM NAD+ Halobacterium salinarum
3 4 D-glutamate pH 8.0, 40°C, Ki(slope), at 120 mM L-glutamate Halobacterium salinarum
5
-
 NADP+ pH 8.0, 40°C, Ki(slope), at 2.5 mM NAD+ Halobacterium salinarum
5.5
-
 2-oxoglutarate pH 8.0, 40°C, Ki(intercept), at 120 mM L-glutamate Halobacterium salinarum
5.7
-
 fumarate pH 8.0, 40°C, Ki(intercept), at 2.5 mM NAD+ Halobacterium salinarum
8.3
-
 succinate pH 8.0, 40°C, Ki(slope), at 2.5 mM NAD+ Halobacterium salinarum
8.4
-
 fumarate pH 8.0, 40°C, Ki(intercept), at 120 mM L-glutamate Halobacterium salinarum
9
-
 NADP+ pH 8.0, 40°C, Ki(intercept), at 2.5 mM NAD+ Halobacterium salinarum
10
-
 2-oxoglutarate pH 8.0, 40°C, Ki(slope), at 120 mM L-glutamate Halobacterium salinarum
18.7
-
 D-glutamate pH 8.0, 40°C, Ki(intercept), at 120 mM L-glutamate Halobacterium salinarum
20
-
 fumarate pH 8.0, 40°C, Ki(slope), at 120 mM L-glutamate Halobacterium salinarum
22
-
 malate pH 8.0, 40°C, Ki(slope), at 2.5 mM NAD+ Halobacterium salinarum
24
-
 succinate pH 8.0, 40°C, Ki(slope), at 120 mM L-glutamate Halobacterium salinarum
26
-
 oxaloacetate pH 8.0, 40°C, Ki(slope), at 2.5 mM NAD+ Halobacterium salinarum
28
-
 succinate pH 8.0, 40°C, Ki(intercept), at 2.5 mM NAD+ Halobacterium salinarum
33
-
 succinate pH 8.0, 40°C, Ki(intercept), at 120 mM L-glutamate Halobacterium salinarum
35
-
 adipate pH 8.0, 40°C, Ki(slope), at 2.5 mM NAD+ Halobacterium salinarum
53
-
 Glutarate pH 8.0, 40°C, Ki(intercept), at 120 mM L-glutamate Halobacterium salinarum
64
-
 malate pH 8.0, 40°C, Ki(slope), at 120 mM L-glutamate Halobacterium salinarum
65
-
 malate pH 8.0, 40°C, Ki(intercept), at 2.5 mM NAD+ Halobacterium salinarum
66
-
 malate pH 8.0, 40°C, Ki(intercept), at 120 mM L-glutamate Halobacterium salinarum
174
-
 oxaloacetate pH 8.0, 40°C, Ki(intercept), at 120 mM L-glutamate Halobacterium salinarum
187
-
 oxaloacetate pH 8.0, 40°C, Ki(slope), at 120 mM L-glutamate Halobacterium salinarum