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Literature summary for 1.4.1.2 extracted from
- Structural characteristics of active and inactive glutamate dehydrogenases from the hyperthermophile Pyrobaculum islandicum (2012), Biosci. Biotechnol. Biochem., 76, 1601-1610.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the enzyme is activated by heat above 70°C, urea and organic solvents, but not by salts. Activation of recombinant enzyme by urea and heat, that is purified as inactive enzyme at 4°C | Pyrobaculum islandicum | |
| Urea | activates the enzyme irreversibly at 5 M | Pyrobaculum islandicum |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of inactive enzyme in Escherichia coli | Pyrobaculum islandicum |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 47000 | - |
6 * 47000, SDS-PAGE | Pyrobaculum islandicum |
| 280000 | - |
- |
Pyrobaculum islandicum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-glutamate + H2O + NAD+ | Pyrobaculum islandicum | - |
2-oxoglutarate + NH3 + NADH + H+ | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pyrobaculum islandicum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| inactivate recombinant enzyme expressed from Escherichia coli by affinity and anion exchange chromatography, followed by gel filtrration, activation by 5 M urea and 70°c | Pyrobaculum islandicum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-glutamate + H2O + NAD+ | - |
Pyrobaculum islandicum | 2-oxoglutarate + NH3 + NADH + H+ | - |
r | |
| L-norvaline + H2O + NAD+ | - |
Pyrobaculum islandicum | 2-oxopentanoate + NH3 + NADH + H+ | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | 6 * 47000, SDS-PAGE | Pyrobaculum islandicum |
| More | three-dimensional structure and structure-activity relationship, modeling, comparison to other hyperthermophilic enzymes from Pyrococcus furiosus and Thermococcus litoralis, overview | Pyrobaculum islandicum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| NAD-dependent glutamate dehydrogenase | - |
Pyrobaculum islandicum |
| NAD-GDH | - |
Pyrobaculum islandicum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 90 | - |
at pH 9.7 | Pyrobaculum islandicum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
the enzyme shows extremely high temperature stability, molecular mechanism and comparison to other hyperthermophilic enzymes from Pyrococcus furiosus and Thermococcus litoralis, overview | Pyrobaculum islandicum |
| 100 | - |
pH 7.2, 120 min, stable | Pyrobaculum islandicum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
amination | Pyrobaculum islandicum |
| 9.7 | - |
deamination | Pyrobaculum islandicum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Pyrobaculum islandicum | |
| NADH | - |
Pyrobaculum islandicum | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | structure-activity relationship, modeling, comparison to other hyperthermophilic enzymes from Pyrococcus furiosus and Thermococcus litoralis, overview | Pyrobaculum islandicum |
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