Protein Variants | Comment | Organism |
---|---|---|
D263K | site-directed mutagenesis, the mutant shows altered binding kinetics for cofactors compared to the wild-type enzyme, the mutant shows increased dissociation constants for NAD+, NADH, and NADPH, but decreased for DTNB leading to inactivation by the inhibitor | [Clostridium] symbiosum |
F238S | site-directed mutagenesis, the mutant shows altered binding kinetics for cofactors compared to the wild-type enzyme, the mutant shows increased dissociation constants | [Clostridium] symbiosum |
F238S/P262S | site-directed mutagenesis, the mutant shows altered binding kinetics for cofactors compared to the wild-type enzyme, the mutant shows increased dissociation constants | [Clostridium] symbiosum |
P262S | site-directed mutagenesis, the mutant shows altered binding kinetics for cofactors compared to the wild-type enzyme, the mutant shows increased dissociation constants | [Clostridium] symbiosum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
DTNB | inactivation via blocking of the only two Cys residues, Cys144 in helix alpha7a of domain I, the substrate-binding domain, and Cys320 in a loop that connects betak and alpha13 in domain II, the coenzyme-binding domain | [Clostridium] symbiosum | |
NADPH | the wrong cofactor, NADPH, without the correct binding pocket to receive its 2'-phosphate, finds an alternative and catalytically unproductive way of occupying the coenzyme site | [Clostridium] symbiosum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | dissociation constants of wild-type and mutant enzymes for different coenzymes, overview | [Clostridium] symbiosum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + H2O + NAD+ | [Clostridium] symbiosum | - |
2-oxoglutarate + NH3 + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
[Clostridium] symbiosum | P24295 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + H2O + NAD+ | - |
[Clostridium] symbiosum | 2-oxoglutarate + NH3 + NADH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GDH | - |
[Clostridium] symbiosum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | [Clostridium] symbiosum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | [Clostridium] symbiosum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
[Clostridium] symbiosum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | pseudo-first-order kinetic plots for inactivation of F238S GDH by DTNB, overview | [Clostridium] symbiosum |