Cloned (Comment) | Organism |
---|---|
expression of wild-type GDH in Escherichia coli strain BL21(DE3), and of the SeMet-labeled enzyme in Escherichia coli strain B834 (DE3) | Peptoniphilus asaccharolyticus |
Crystallization (Comment) | Organism |
---|---|
purified recombinant wild-type and SeMet-labeled GDHs, hanging drop vapour diffusion method, at 20°C, protein in 10 mM Tris-HCl, pH 7.0, mixing of 0.002 ml of protein solution with 0.001 ml of reservoir solution containing 2.0 M ammonium sulfate, 0.1 M sodium cacodylate, pH 6.5, 200 mM NaCl, and equilibration with 0.5 ml reservoir solution, X-ray diffraction structure determination and analysis at 3.5 A resolution | Peptoniphilus asaccharolyticus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + H2O + NAD+ | Peptoniphilus asaccharolyticus | - |
2-oxoglutarate + NH3 + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Peptoniphilus asaccharolyticus | P28997 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + H2O + NAD+ | - |
Peptoniphilus asaccharolyticus | 2-oxoglutarate + NH3 + NADH + H+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
GDH | - |
Peptoniphilus asaccharolyticus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | NADP+ and NADPH are poor cofactors. The presence of an acidic residue at the P7 position, adjacent to the 2'-OH group, typically discriminates against NADP+. PaGDH contains a P7 glutamate and has high specificity for NAD(H), with a kcat/Km for NAD+ that is approximately 1000fold greater than that for NADP+ | Peptoniphilus asaccharolyticus | |
NAD+ | - |
Peptoniphilus asaccharolyticus | |
NADH | - |
Peptoniphilus asaccharolyticus |