Literature summary for 1.4.1.2 extracted from

Newbold, C.J.; McEwan, N.R.; Calza, R.E.; Chareyron, E.N.; Duval, S.M.; Eschenlauer, S.C.; McIntosh, F.M.; Nelson, N.; Travis, A.J.; Wallace, R.J.
An NAD(+)-dependent glutamate dehydrogenase cloned from the ruminal ciliate protozoan, Entodinium caudatum (2005), FEMS Microbiol. Lett., 247, 113-121.

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Application

Application	Comment	Organism
additional information	high sequence similarity to GDH genes from the Bacteroides, GDH is an anabolic enzyme catalysing the assimilation of ammonia by Entodinium caudatum in the rumen, the gene is probably acquired by lateral gene transfer from a ruminal bacterium	Entodinium caudatum

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli XLOLR	Entodinium caudatum

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.71	-	2-oxoglutarate	-	Entodinium caudatum
2.33	-	NH4+	-	Entodinium caudatum
98	-	L-glutamate	-	Entodinium caudatum

Metals/Ions

Metals/Ions	Comment	Organism	Structure
additional information	high K+ concentrations up to 0.45 M have no influence on activity	Entodinium caudatum
NH4+	transcriptional upregulation	Entodinium caudatum

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
48000	-	sequence analysis	Entodinium caudatum

Organism

Organism	UniProt	Comment	Textmining
Entodinium caudatum	Q9TVN3	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.077	-	NADP-linked GDH activity	Entodinium caudatum
0.55	-	-	Entodinium caudatum
0.77	-	NAD-linked GDH activity	Entodinium caudatum
0.97	-	in the presence of antibiotics and ammonia	Entodinium caudatum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-oxoglutarate + NADH + NH3	-	Entodinium caudatum	L-glutamate + NAD+ + H2O	-	r
2-oxoglutarate + NH4+ + NADPH	-	Entodinium caudatum	L-glutamate + NADP+	-	?

Synonyms

Synonyms	Comment	Organism
GDH	-	Entodinium caudatum
NAD(+)-dependent glutamate dehydrogenase	-	Entodinium caudatum

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	-	Entodinium caudatum

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	-	Entodinium caudatum

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Release 2023.1 (January 2023)