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Literature summary for 1.4.1.2 extracted from

  • Pasquo, A.; Britton, K.L.; Stillman, T.J.; Rice, D.W.; Coelfen, H.; Harding, S.E.; Scandurra, R.; Engel, P.C.
    Construction of a dimeric form of glutamate dehydrogenase from Clostridium symbiosum by site-directed mutagenesis (1996), Biochim. Biophys. Acta, 1297, 149-158.
    View publication on PubMed
Search for more literature for 1.4.1.2

Protein Variants

Protein Variants Comment Organism
F187D dimeric form of enzyme [Clostridium] symbiosum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47000
-
 F187D mutant, 2 * 47000 [Clostridium] symbiosum
87000
-
 F187D mutant, gel filtration [Clostridium] symbiosum

Organism

Organism UniProt Comment Textmining
[Clostridium] symbiosum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
overexpression of F187D mutant in Escherichia coli [Clostridium] symbiosum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoglutarate + NH3 + NADH
-
 [Clostridium] symbiosum L-glutamate + H2O + NAD+
-
 r
L-glutamate + H2O + NAD+
-
 [Clostridium] symbiosum 2-oxoglutarate + NH3 + NADH + H+
-
 r

Subunits

Subunits Comment Organism
dimer F187D mutant, 2 * 47000 [Clostridium] symbiosum

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 [Clostridium] symbiosum