Literature summary for 1.4.1.2 extracted from

Anderson, B.M.; Anderson, C.D.; Van Tassell, R.L.; Lyerly, D.M.; Wilkins, T.D.
Purification and characterization of Clostridium difficile glutamate dehydrogenase (1993), Arch. Biochem. Biophys., 300, 483-488.

Search for more literature for 1.4.1.2

Inhibitors

Inhibitors	Comment	Organism	Structure
ADP	-	Clostridioides difficile
AMP	-	Clostridioides difficile

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.8	-	NAD+	-	Clostridioides difficile
4.3	-	L-glutamate	-	Clostridioides difficile

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
45000	-	6 * 45000, SDS-PAGE	Clostridioides difficile
300000	-	gel filtration	Clostridioides difficile

Organism

Organism	UniProt	Comment	Textmining
Clostridioides difficile	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
overexpression in Escherichia coli	Clostridioides difficile

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
146	-	-	Clostridioides difficile

Storage Stability

Storage Stability	Organism
-15°C, 50 mM Tris-HCl, pH 7.5, 0.1 M KCl, stable for several months	Clostridioides difficile

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-oxoglutarate + NH3 + NADH	-	Clostridioides difficile	L-glutamate + H2O + NAD+	-	r
L-glutamate + H2O + NAD+	-	Clostridioides difficile	2-oxoglutarate + NH3 + NADH + H+	-	r

Subunits

Subunits	Comment	Organism
hexamer	6 * 45000, SDS-PAGE	Clostridioides difficile

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8.5	-	-	Clostridioides difficile

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	-	Clostridioides difficile

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Release 2023.1 (January 2023)