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Literature summary for 1.4.1.18 extracted from

  • Misono, H.; Hashimoto, H.; Uehigashi, H.; Nagata, S.; Nagasaki, S.
    Properties of L-lysine epsilon-dehydrogenase from Agrobacterium tumefaciens (1989), J. Biochem., 105, 1002-1008.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
L-lysine increased activity after 10 min preincubation Agrobacterium tumefaciens

General Stability

General Stability Organism
L-lysine stabilizes Agrobacterium tumefaciens

Inhibitors

Inhibitors Comment Organism Structure
DTNB almost complete inhibition in presence of NAD+, 40% inhibition in presence of L-lysine Agrobacterium tumefaciens
HgCl2 complete inhibition at 0.001 mM after 10 min incubation Agrobacterium tumefaciens
N-ethylmaleimide complete inhibition at 1 mM after 10 min incubation Agrobacterium tumefaciens
p-chloromercuribenzoate complete inhibition at 0.01 mM after 10 min incubation, reversible with 2-mercaptoethanol or dithiothreitol Agrobacterium tumefaciens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.059
-
NAD+
-
Agrobacterium tumefaciens
1.85
-
deamino-NAD+
-
Agrobacterium tumefaciens
3.13
-
3-acetylpyridine-NAD+
-
Agrobacterium tumefaciens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
160000
-
ultracentrifugation in presence of L-lysine Agrobacterium tumefaciens
165000
-
gel filtration in presence of L-lysine Agrobacterium tumefaciens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-lysine + NAD+ + H2O Agrobacterium tumefaciens
-
alpha-aminoadipate delta-semialdehyde + NH3 + NADH product is spontaneosly converted into delta1-piperideine-6-carboxylate ?

Organism

Organism UniProt Comment Textmining
Agrobacterium tumefaciens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Agrobacterium tumefaciens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.5
-
preincubation in absence of L-lysine Agrobacterium tumefaciens
15.7
-
preincubation in presence of L-lysine Agrobacterium tumefaciens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-lysine + 3-acetylpyridine-NAD+ + H2O
-
Agrobacterium tumefaciens alpha-aminoadipate delta-semialdehyde + NH3 + 3-acetylpyridine-NADH
-
?
L-lysine + deamino-NAD+ + H2O
-
Agrobacterium tumefaciens alpha-aminoadipate delta-semialdehyde + NH3 + deamino-NADH
-
?
L-lysine + NAD+ + H2O
-
Agrobacterium tumefaciens alpha-aminoadipate delta-semialdehyde + NH3 + NADH product is spontaneosly converted into delta1-piperideine-6-carboxylate ?
S-(beta-aminoethyl)-L-cysteine + NAD+ 3% of the activity with L-lysine Agrobacterium tumefaciens ?
-
?

Subunits

Subunits Comment Organism
dimer after preincubation with NAD+ without L-lysine Agrobacterium tumefaciens
tetramer gel filtration and centrifugation in presence of L-lysine Agrobacterium tumefaciens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
stable for 10 min in absence of L-lysine, unstable above 40°C Agrobacterium tumefaciens
50
-
stable for 10 min in presence of L-lysine, unstable above 50°C Agrobacterium tumefaciens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.7
-
-
Agrobacterium tumefaciens

Cofactor

Cofactor Comment Organism Structure
3-acetylpyridine-NAD+ 16% of activity compared with NAD+ Agrobacterium tumefaciens
deamino-NAD+ 42% of activity compared with NAD+ Agrobacterium tumefaciens
NAD+
-
Agrobacterium tumefaciens