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Literature summary for 1.4.1.16 extracted from

  • Xu, J.Z.; Ruan, H.Z.; Liu, L.M.; Wang, L.P.; Zhang, W.G.
    Overexpression of thermostable meso-diaminopimelate dehydrogenase to redirect diaminopimelate pathway for increasing L-lysine production in Escherichia coli (2019), Sci. Rep., 9, 2423 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.4.1.16

Application

Application Comment Organism
synthesis expression of DapDH in Escherichia coli significantly enhances carbon flux into the pentose phosphate pathway and L-lysine biosynthetic pathway, thus increasing the levels of NADPH and precursors for L-lysine biosynthesis. The coexistence of two DAP-pathways and sufficient ammonium availability are good for increasing the final titer of L-lysine with a high carbon yield and productivity in Escherichia coli. Fed-batch fermentation of the target strain results in 119.5 g/l of L-lysine with a carbon yield of 49.1% and productivity of 2.99 g per l and h Symbiobacterium thermophilum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.11
-
 L-DELTA1-tetrahydrodipicolinate pH 8.5, 30°C Acetivibrio thermocellus
0.11
-
 meso-2,6-diaminoheptanedioate pH 10, 30°C Bacteroides fragilis
0.21
-
 meso-2,6-diaminoheptanedioate pH 10, 30°C Acetivibrio thermocellus
0.23
-
 L-DELTA1-tetrahydrodipicolinate pH 8.5, 30°C Lysinibacillus sphaericus
0.24
-
 L-DELTA1-tetrahydrodipicolinate pH 8.5, 30°C Symbiobacterium thermophilum
0.26
-
 L-DELTA1-tetrahydrodipicolinate pH 8.5, 30°C Ureibacillus thermosphaericus
0.27
-
 L-DELTA1-tetrahydrodipicolinate pH 8.5, 30°C Corynebacterium glutamicum
0.57
-
 L-DELTA1-tetrahydrodipicolinate pH 8.5, 30°C Bacteroides fragilis
1.8
-
 meso-2,6-diaminoheptanedioate pH 10, 30°C Symbiobacterium thermophilum
1.9
-
 meso-2,6-diaminoheptanedioate pH 10, 30°C Ureibacillus thermosphaericus
2.4
-
 meso-2,6-diaminoheptanedioate pH 10, 30°C Lysinibacillus sphaericus
2.8
-
 meso-2,6-diaminoheptanedioate pH 10, 30°C Corynebacterium glutamicum
4.2
-
 NH4+ pH 8.5, 30°C Bacteroides fragilis
6.3
-
 NH4+ pH 8.5, 30°C Symbiobacterium thermophilum
6.5
-
 NH4+ pH 8.5, 30°C Ureibacillus thermosphaericus
8 9 NH4+ pH 8.5, 30°C Acetivibrio thermocellus
10.8
-
 NH4+ pH 8.5, 30°C Lysinibacillus sphaericus
39.2
-
 NH4+ pH 8.5, 30°C Corynebacterium glutamicum

Organism

Organism UniProt Comment Textmining
Acetivibrio thermocellus A3DDX7
-
-
Acetivibrio thermocellus ATCC 27405 A3DDX7
-
-
Bacteroides fragilis Q64PZ8
-
-
Bacteroides fragilis YCH46 Q64PZ8
-
-
Corynebacterium glutamicum P04964
-
-
Corynebacterium glutamicum ATCC 13032 P04964
-
-
Lysinibacillus sphaericus Q9KWR0
-
-
Symbiobacterium thermophilum Q67PI3
-
-
Ureibacillus thermosphaericus G1UII1
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-DELTA1-tetrahydrodipicolinate + NH4+ + NADPH
-
 Corynebacterium glutamicum meso-2,6-diaminoheptanedioate + NADP+
-
 ?
L-DELTA1-tetrahydrodipicolinate + NH4+ + NADPH
-
 Symbiobacterium thermophilum meso-2,6-diaminoheptanedioate + NADP+
-
 ?
L-DELTA1-tetrahydrodipicolinate + NH4+ + NADPH
-
 Ureibacillus thermosphaericus meso-2,6-diaminoheptanedioate + NADP+
-
 ?
L-DELTA1-tetrahydrodipicolinate + NH4+ + NADPH
-
 Lysinibacillus sphaericus meso-2,6-diaminoheptanedioate + NADP+
-
 ?
L-DELTA1-tetrahydrodipicolinate + NH4+ + NADPH
-
 Acetivibrio thermocellus meso-2,6-diaminoheptanedioate + NADP+
-
 ?
L-DELTA1-tetrahydrodipicolinate + NH4+ + NADPH
-
 Bacteroides fragilis meso-2,6-diaminoheptanedioate + NADP+
-
 ?
L-DELTA1-tetrahydrodipicolinate + NH4+ + NADPH
-
 Bacteroides fragilis YCH46 meso-2,6-diaminoheptanedioate + NADP+
-
 ?
L-DELTA1-tetrahydrodipicolinate + NH4+ + NADPH
-
 Corynebacterium glutamicum ATCC 13032 meso-2,6-diaminoheptanedioate + NADP+
-
 ?
L-DELTA1-tetrahydrodipicolinate + NH4+ + NADPH
-
 Acetivibrio thermocellus ATCC 27405 meso-2,6-diaminoheptanedioate + NADP+
-
 ?
meso-2,6-diaminoheptanedioate + NADP+
-
 Corynebacterium glutamicum L-DELTA1-tetrahydrodipicolinate + NH4+ + NADPH
-
 ?
meso-2,6-diaminoheptanedioate + NADP+
-
 Symbiobacterium thermophilum L-DELTA1-tetrahydrodipicolinate + NH4+ + NADPH
-
 ?
meso-2,6-diaminoheptanedioate + NADP+
-
 Ureibacillus thermosphaericus L-DELTA1-tetrahydrodipicolinate + NH4+ + NADPH
-
 ?
meso-2,6-diaminoheptanedioate + NADP+
-
 Lysinibacillus sphaericus L-DELTA1-tetrahydrodipicolinate + NH4+ + NADPH
-
 ?
meso-2,6-diaminoheptanedioate + NADP+
-
 Acetivibrio thermocellus L-DELTA1-tetrahydrodipicolinate + NH4+ + NADPH
-
 ?
meso-2,6-diaminoheptanedioate + NADP+
-
 Bacteroides fragilis L-DELTA1-tetrahydrodipicolinate + NH4+ + NADPH
-
 ?
meso-2,6-diaminoheptanedioate + NADP+
-
 Bacteroides fragilis YCH46 L-DELTA1-tetrahydrodipicolinate + NH4+ + NADPH
-
 ?
meso-2,6-diaminoheptanedioate + NADP+
-
 Corynebacterium glutamicum ATCC 13032 L-DELTA1-tetrahydrodipicolinate + NH4+ + NADPH
-
 ?
meso-2,6-diaminoheptanedioate + NADP+
-
 Acetivibrio thermocellus ATCC 27405 L-DELTA1-tetrahydrodipicolinate + NH4+ + NADPH
-
 ?

Synonyms

Synonyms Comment Organism
BAB07799
-
 Lysinibacillus sphaericus
BF3690
-
 Bacteroides fragilis
Sth1425
-
 Symbiobacterium thermophilum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.9
-
 L-DELTA1-tetrahydrodipicolinate pH 8.5, 30°C Bacteroides fragilis
7.7
-
 meso-2,6-diaminoheptanedioate pH 10, 30°C Bacteroides fragilis
41.9
-
 L-DELTA1-tetrahydrodipicolinate pH 8.5, 30°C Corynebacterium glutamicum
50.6
-
 L-DELTA1-tetrahydrodipicolinate pH 8.5, 30°C Ureibacillus thermosphaericus
52.8
-
 L-DELTA1-tetrahydrodipicolinate pH 8.5, 30°C Symbiobacterium thermophilum
64.7
-
 L-DELTA1-tetrahydrodipicolinate pH 8.5, 30°C Lysinibacillus sphaericus
115.4
-
 meso-2,6-diaminoheptanedioate pH 10, 30°C Corynebacterium glutamicum
123.3
-
 meso-2,6-diaminoheptanedioate pH 10, 30°C Ureibacillus thermosphaericus
145.7
-
 meso-2,6-diaminoheptanedioate pH 10, 30°C Symbiobacterium thermophilum
154.2
-
 meso-2,6-diaminoheptanedioate pH 10, 30°C Lysinibacillus sphaericus
160.8
-
 NH4+ pH 8.5, 30°C Bacteroides fragilis
278.2
-
 L-DELTA1-tetrahydrodipicolinate pH 8.5, 30°C Acetivibrio thermocellus
344.3
-
 meso-2,6-diaminoheptanedioate pH 10, 30°C Acetivibrio thermocellus
347.7
-
 NH4+ pH 8.5, 30°C Ureibacillus thermosphaericus
387.4
-
 NH4+ pH 8.5, 30°C Symbiobacterium thermophilum
511.9
-
 NH4+ pH 8.5, 30°C Lysinibacillus sphaericus
768.3
-
 NH4+ pH 8.5, 30°C Corynebacterium glutamicum
1815.6
-
 NH4+ pH 8.5, 30°C Acetivibrio thermocellus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
8.6
-
 L-DELTA1-tetrahydrodipicolinate pH 8.5, 30°C Bacteroides fragilis
23.8
-
 NH4+ pH 8.5, 30°C Corynebacterium glutamicum
38.3
-
 NH4+ pH 8.5, 30°C Bacteroides fragilis
41.2
-
 meso-2,6-diaminoheptanedioate pH 10, 30°C Corynebacterium glutamicum
47.4
-
 NH4+ pH 8.5, 30°C Lysinibacillus sphaericus
53.4
-
 NH4+ pH 8.5, 30°C Ureibacillus thermosphaericus
61.5
-
 NH4+ pH 8.5, 30°C Symbiobacterium thermophilum
64.3
-
 meso-2,6-diaminoheptanedioate pH 10, 30°C Lysinibacillus sphaericus
64.9
-
 meso-2,6-diaminoheptanedioate pH 10, 30°C Ureibacillus thermosphaericus
70
-
 meso-2,6-diaminoheptanedioate pH 10, 30°C Bacteroides fragilis
80.9
-
 meso-2,6-diaminoheptanedioate pH 10, 30°C Symbiobacterium thermophilum
155
-
 L-DELTA1-tetrahydrodipicolinate pH 8.5, 30°C Corynebacterium glutamicum
194.6
-
 L-DELTA1-tetrahydrodipicolinate pH 8.5, 30°C Ureibacillus thermosphaericus
205.4
-
 NH4+ pH 8.5, 30°C Acetivibrio thermocellus
220
-
 L-DELTA1-tetrahydrodipicolinate pH 8.5, 30°C Symbiobacterium thermophilum
281.3
-
 L-DELTA1-tetrahydrodipicolinate pH 8.5, 30°C Lysinibacillus sphaericus
1638.6
-
 meso-2,6-diaminoheptanedioate pH 10, 30°C Acetivibrio thermocellus
2318.3
-
 L-DELTA1-tetrahydrodipicolinate pH 8.5, 30°C Acetivibrio thermocellus