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Literature summary for 1.4.1.16 extracted from

  • Wu, W.; Zhang, Y.; Liu, D.; Chen, Z.
    Efficient mining of natural NADH-utilizing dehydrogenases enables systematic cofactor engineering of lysine synthesis pathway of Corynebacterium glutamicum (2019), Metab. Eng., 52, 77-86 .
    View publication on PubMed
Search for more literature for 1.4.1.16

Application

Application Comment Organism
synthesis individual overexpression of ASPDH, aspartate-semialdehyde dehydrogenase from Tistrella mobilis, dihydrodipicolinate reductase from Escherichia coli, and diaminopimelate dehydrogenase from Pseudothermotoga thermarum in Corynebacterium glutamicum LC298, a basic lysine producer, increases the production of lysine by 30.7%, 32.4%, 17.4%, and 36.8%, respectively. The highest increase of lysine production (30.7%) is observed for a triple-mutant strain (27.7 g/L, 0.35 g/g glucose) expressing ASPDH, aspartate-semialdehyde dehydrogenase from Tistrella mobilis, dihydrodipicolinate reductase from Escherichia coli. A quadruple-mutant strain expressing all of the four NADH-utilizing enzymes allows high lysine production (24.1 g/l, 0.30 g/g glucose) almost independent of the oxidative pentose phosphate pathway Pseudothermotoga thermarum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.18
-
 NADH pH 8.2, 37°C Pseudothermotoga thermarum
0.41
-
 NADPH pH 8.2, 37°C Pseudothermotoga thermarum

Organism

Organism UniProt Comment Textmining
Pseudothermotoga thermarum F7YTT4
-
-
Pseudothermotoga thermarum DSM 5069 F7YTT4
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-2-amino-6-oxoheptanedioate + H+ + NADH + NH4+
-
 Pseudothermotoga thermarum meso-2,6-diaminoheptanedioate + H2O + NAD+
-
 ?
(S)-2-amino-6-oxoheptanedioate + H+ + NADH + NH4+
-
 Pseudothermotoga thermarum DSM 5069 meso-2,6-diaminoheptanedioate + H2O + NAD+
-
 ?
(S)-2-amino-6-oxoheptanedioate + H+ + NADPH + NH4+
-
 Pseudothermotoga thermarum meso-2,6-diaminoheptanedioate + H2O + NADP+
-
 ?
(S)-2-amino-6-oxoheptanedioate + H+ + NADPH + NH4+
-
 Pseudothermotoga thermarum DSM 5069 meso-2,6-diaminoheptanedioate + H2O + NADP+
-
 ?

Synonyms

Synonyms Comment Organism
Theth_1310
-
 Pseudothermotoga thermarum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.73
-
 NADPH pH 8.2, 37°C Pseudothermotoga thermarum
5.97
-
 NADH pH 8.2, 37°C Pseudothermotoga thermarum

Cofactor

Cofactor Comment Organism Structure
NAD+ catalytic efficiency ratio of NAD+/NADP+ is 18.6 Pseudothermotoga thermarum
NADP+ catalytic efficiency ratio of NAD+/NADP+ is 18.6 Pseudothermotoga thermarum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.8
-
 NADPH pH 8.2, 37°C Pseudothermotoga thermarum
33.2
-
 NADH pH 8.2, 37°C Pseudothermotoga thermarum