Crystallization (Comment) | Organism |
---|---|
molecular docking of substrates meso-DAP and pyruvic acid to wild-type and mutant R71A. Substrate binding with meso-DAP or pyruvic acid changes the rigidity of the enzyme and the secondary structure around R71 | Symbiobacterium thermophilum |
Protein Variants | Comment | Organism |
---|---|---|
A69R | mutation to the correspondung residue of Symbiobacteium thermophilum DAPDH. Mutation improves the catalytic efficiencies toward 2-keto acids and does not affect the catalytic efficiency toward meso-DAP | Corynebacterium glutamicum |
R71A | mutation destroys the cation-pi interaction between residues R71 and Y205 | Symbiobacterium thermophilum |
R71E | for reductive amination, the mutant shows a 1.5fold increase in Km and 0.24fold decrease in kcat. For oxidative deamination, a 50% decrease in the kcat/Km value is observed | Symbiobacterium thermophilum |
R71I | R71I shows 0.37fold decrease in kcat/Km value for pyruvic acid | Symbiobacterium thermophilum |
R71K | the kcat/Km values toward pyruvic acid and meso-DAP increase by 1.4fold and 1.8fold, respectively | Symbiobacterium thermophilum |
R71Q | the kcat/Km values toward pyruvic acid and meso-DAP both decrease by 30% | Symbiobacterium thermophilum |
R71S | for pyruvic acid, R71S shows few changes in kcat/Km values | Symbiobacterium thermophilum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.41 | - |
meso-2,6-diaminoheptanedioate | wild-type, pH not specified in the publication, temperature not specified in the publication | Symbiobacterium thermophilum | |
0.43 | - |
meso-2,6-diaminoheptanedioate | wild-type, pH not specified in the publication, temperature not specified in the publication | Corynebacterium glutamicum | |
0.43 | - |
meso-2,6-diaminoheptanedioate | mutant R71S, pH not specified in the publication, temperature not specified in the publication | Symbiobacterium thermophilum | |
0.53 | - |
meso-2,6-diaminoheptanedioate | mutant A69R, pH not specified in the publication, temperature not specified in the publication | Corynebacterium glutamicum | |
0.56 | - |
meso-2,6-diaminoheptanedioate | mutant R71I, pH not specified in the publication, temperature not specified in the publication | Symbiobacterium thermophilum | |
0.59 | - |
meso-2,6-diaminoheptanedioate | mutant R71K, pH not specified in the publication, temperature not specified in the publication | Symbiobacterium thermophilum | |
0.63 | - |
meso-2,6-diaminoheptanedioate | mutant R71Q, pH not specified in the publication, temperature not specified in the publication | Symbiobacterium thermophilum | |
1.37 | - |
meso-2,6-diaminoheptanedioate | mutant R71E, pH not specified in the publication, temperature not specified in the publication | Symbiobacterium thermophilum | |
10.91 | - |
pyruvate | mutant R71K, pH not specified in the publication, temperature not specified in the publication | Symbiobacterium thermophilum | |
14.08 | - |
pyruvate | mutant R71S, pH not specified in the publication, temperature not specified in the publication | Symbiobacterium thermophilum | |
16.31 | - |
pyruvate | wild-type, pH not specified in the publication, temperature not specified in the publication | Symbiobacterium thermophilum | |
19.17 | - |
pyruvate | mutant R71Q, pH not specified in the publication, temperature not specified in the publication | Symbiobacterium thermophilum | |
19.24 | - |
pyruvate | mutant R71I, pH not specified in the publication, temperature not specified in the publication | Symbiobacterium thermophilum | |
25.18 | - |
pyruvate | mutant R71E, pH not specified in the publication, temperature not specified in the publication | Symbiobacterium thermophilum | |
26.88 | - |
4-methyl-2-oxopentanoate | mutant A69R, pH not specified in the publication, temperature not specified in the publication | Corynebacterium glutamicum | |
29.92 | - |
oxaloacetate | wild-type, pH not specified in the publication, temperature not specified in the publication | Corynebacterium glutamicum | |
31.5 | - |
oxaloacetate | mutant A69R, pH not specified in the publication, temperature not specified in the publication | Corynebacterium glutamicum | |
33.51 | - |
4-methyl-2-oxopentanoate | wild-type, pH not specified in the publication, temperature not specified in the publication | Corynebacterium glutamicum | |
36.16 | - |
2-oxovalerate | mutant A69R, pH not specified in the publication, temperature not specified in the publication | Corynebacterium glutamicum | |
57.04 | - |
2-oxovalerate | wild-type, pH not specified in the publication, temperature not specified in the publication | Corynebacterium glutamicum | |
97.88 | - |
3-methyl-2-oxobutanoate | wild-type, pH not specified in the publication, temperature not specified in the publication | Corynebacterium glutamicum | |
119.2 | - |
3-methyl-2-oxobutanoate | mutant A69R, pH not specified in the publication, temperature not specified in the publication | Corynebacterium glutamicum | |
294.9 | - |
pyruvate | wild-type, pH not specified in the publication, temperature not specified in the publication | Corynebacterium glutamicum | |
337.6 | - |
pyruvate | mutant A69R, pH not specified in the publication, temperature not specified in the publication | Corynebacterium glutamicum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Corynebacterium glutamicum | P04964 | - |
- |
Corynebacterium glutamicum ATCC 13032 | P04964 | - |
- |
Symbiobacterium thermophilum | Q67PI3 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxovalerate + NH3 + NADPH + H+ | - |
Corynebacterium glutamicum | ? + H2O + NADP+ | - |
? | |
2-oxovalerate + NH3 + NADPH + H+ | - |
Corynebacterium glutamicum ATCC 13032 | ? + H2O + NADP+ | - |
? | |
3-methyl-2-oxobutanoate + NH3 + NADPH + H+ | - |
Corynebacterium glutamicum | ? + H2O + NADP+ | - |
? | |
3-methyl-2-oxobutanoate + NH3 + NADPH + H+ | - |
Corynebacterium glutamicum ATCC 13032 | ? + H2O + NADP+ | - |
? | |
4-methyl-2-oxopentanoate + NH3 + NADPH + H+ | - |
Corynebacterium glutamicum | ? + H2O + NADP+ | - |
? | |
4-methyl-2-oxopentanoate + NH3 + NADPH + H+ | - |
Corynebacterium glutamicum ATCC 13032 | ? + H2O + NADP+ | - |
? | |
meso-2,6-diaminoheptanedioate + H2O + NADP+ | - |
Corynebacterium glutamicum | L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+ | - |
? | |
meso-2,6-diaminoheptanedioate + H2O + NADP+ | - |
Symbiobacterium thermophilum | L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+ | - |
? | |
meso-2,6-diaminoheptanedioate + H2O + NADP+ | - |
Corynebacterium glutamicum ATCC 13032 | L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+ | - |
? | |
oxaloacetate + NH3 + NADPH + H+ | - |
Corynebacterium glutamicum | ? + H2O + NADP+ | - |
? | |
oxaloacetate + NH3 + NADPH + H+ | - |
Corynebacterium glutamicum ATCC 13032 | ? + H2O + NADP+ | - |
? | |
pyruvate + NH3 + NADPH + H+ | - |
Corynebacterium glutamicum | D-alanine + H2O + NADP+ | - |
? | |
pyruvate + NH3 + NADPH + H+ | - |
Symbiobacterium thermophilum | D-alanine + H2O + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DAPDH | - |
Corynebacterium glutamicum |
DAPDH | - |
Symbiobacterium thermophilum |
DDH | - |
Corynebacterium glutamicum |
Sth1425 | - |
Symbiobacterium thermophilum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.087 | - |
oxaloacetate | wild-type, pH not specified in the publication, temperature not specified in the publication | Corynebacterium glutamicum | |
0.1 | - |
4-methyl-2-oxopentanoate | mutant A69R, pH not specified in the publication, temperature not specified in the publication | Corynebacterium glutamicum | |
0.11 | - |
oxaloacetate | mutant A69R, pH not specified in the publication, temperature not specified in the publication | Corynebacterium glutamicum | |
0.12 | - |
4-methyl-2-oxopentanoate | wild-type, pH not specified in the publication, temperature not specified in the publication | Corynebacterium glutamicum | |
0.27 | - |
3-methyl-2-oxobutanoate | wild-type, pH not specified in the publication, temperature not specified in the publication | Corynebacterium glutamicum | |
0.38 | - |
3-methyl-2-oxobutanoate | mutant A69R, pH not specified in the publication, temperature not specified in the publication | Corynebacterium glutamicum | |
0.91 | - |
2-oxovalerate | mutant A69R, pH not specified in the publication, temperature not specified in the publication | Corynebacterium glutamicum | |
0.93 | - |
2-oxovalerate | wild-type, pH not specified in the publication, temperature not specified in the publication | Corynebacterium glutamicum | |
1.35 | - |
pyruvate | wild-type, pH not specified in the publication, temperature not specified in the publication | Corynebacterium glutamicum | |
1.68 | - |
pyruvate | mutant R71E, pH not specified in the publication, temperature not specified in the publication | Symbiobacterium thermophilum | |
2.27 | - |
pyruvate | mutant A69R, pH not specified in the publication, temperature not specified in the publication | Corynebacterium glutamicum | |
3.09 | - |
pyruvate | mutant R71I, pH not specified in the publication, temperature not specified in the publication | Symbiobacterium thermophilum | |
6.15 | - |
pyruvate | mutant R71Q, pH not specified in the publication, temperature not specified in the publication | Symbiobacterium thermophilum | |
6.45 | - |
pyruvate | mutant R71K, pH not specified in the publication, temperature not specified in the publication | Symbiobacterium thermophilum | |
7 | - |
pyruvate | wild-type, pH not specified in the publication, temperature not specified in the publication | Symbiobacterium thermophilum | |
7.2 | - |
pyruvate | mutant R71S, pH not specified in the publication, temperature not specified in the publication | Symbiobacterium thermophilum | |
37.44 | - |
meso-2,6-diaminoheptanedioate | mutant R71S, pH not specified in the publication, temperature not specified in the publication | Symbiobacterium thermophilum | |
40.47 | - |
meso-2,6-diaminoheptanedioate | wild-type, pH not specified in the publication, temperature not specified in the publication | Symbiobacterium thermophilum | |
45.77 | - |
meso-2,6-diaminoheptanedioate | mutant R71Q, pH not specified in the publication, temperature not specified in the publication | Symbiobacterium thermophilum | |
50.96 | - |
meso-2,6-diaminoheptanedioate | mutant R71I, pH not specified in the publication, temperature not specified in the publication | Symbiobacterium thermophilum | |
51.99 | - |
meso-2,6-diaminoheptanedioate | wild-type, pH not specified in the publication, temperature not specified in the publication | Corynebacterium glutamicum | |
61.58 | - |
meso-2,6-diaminoheptanedioate | mutant A69R, pH not specified in the publication, temperature not specified in the publication | Corynebacterium glutamicum | |
65.62 | - |
meso-2,6-diaminoheptanedioate | mutant R71E, pH not specified in the publication, temperature not specified in the publication | Symbiobacterium thermophilum | |
163.6 | - |
meso-2,6-diaminoheptanedioate | mutant R71K, pH not specified in the publication, temperature not specified in the publication | Symbiobacterium thermophilum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0028 | - |
3-methyl-2-oxobutanoate | wild-type, pH not specified in the publication, temperature not specified in the publication | Corynebacterium glutamicum | |
0.0029 | - |
oxaloacetate | wild-type, pH not specified in the publication, temperature not specified in the publication | Corynebacterium glutamicum | |
0.0034 | - |
4-methyl-2-oxopentanoate | wild-type, pH not specified in the publication, temperature not specified in the publication | Corynebacterium glutamicum | |
0.0046 | - |
pyruvate | wild-type, pH not specified in the publication, temperature not specified in the publication | Corynebacterium glutamicum | |
0.016 | - |
2-oxovalerate | wild-type, pH not specified in the publication, temperature not specified in the publication | Corynebacterium glutamicum | |
0.07 | - |
pyruvate | mutant R71E, pH not specified in the publication, temperature not specified in the publication | Symbiobacterium thermophilum | |
0.16 | - |
pyruvate | mutant R71I, pH not specified in the publication, temperature not specified in the publication | Symbiobacterium thermophilum | |
0.32 | - |
pyruvate | mutant R71Q, pH not specified in the publication, temperature not specified in the publication | Symbiobacterium thermophilum | |
0.43 | - |
pyruvate | wild-type, pH not specified in the publication, temperature not specified in the publication | Symbiobacterium thermophilum | |
0.51 | - |
pyruvate | mutant R71S, pH not specified in the publication, temperature not specified in the publication | Symbiobacterium thermophilum | |
0.59 | - |
pyruvate | mutant R71K, pH not specified in the publication, temperature not specified in the publication | Symbiobacterium thermophilum | |
0.96 | - |
meso-2,6-diaminoheptanedioate | mutant A69R, pH not specified in the publication, temperature not specified in the publication | Corynebacterium glutamicum | |
1.14 | - |
3-methyl-2-oxobutanoate | mutant A69R, pH not specified in the publication, temperature not specified in the publication | Corynebacterium glutamicum | |
1.15 | - |
4-methyl-2-oxopentanoate | mutant A69R, pH not specified in the publication, temperature not specified in the publication | Corynebacterium glutamicum | |
1.24 | - |
oxaloacetate | mutant A69R, pH not specified in the publication, temperature not specified in the publication | Corynebacterium glutamicum | |
1.46 | - |
pyruvate | mutant A69R, pH not specified in the publication, temperature not specified in the publication | Corynebacterium glutamicum | |
1.57 | - |
2-oxovalerate | mutant A69R, pH not specified in the publication, temperature not specified in the publication | Corynebacterium glutamicum | |
47.83 | - |
meso-2,6-diaminoheptanedioate | mutant R71E, pH not specified in the publication, temperature not specified in the publication | Symbiobacterium thermophilum | |
72.68 | - |
meso-2,6-diaminoheptanedioate | mutant R71Q, pH not specified in the publication, temperature not specified in the publication | Symbiobacterium thermophilum | |
86.73 | - |
meso-2,6-diaminoheptanedioate | mutant R71S, pH not specified in the publication, temperature not specified in the publication | Symbiobacterium thermophilum | |
90.84 | - |
meso-2,6-diaminoheptanedioate | mutant R71I, pH not specified in the publication, temperature not specified in the publication | Symbiobacterium thermophilum | |
98.48 | - |
meso-2,6-diaminoheptanedioate | wild-type, pH not specified in the publication, temperature not specified in the publication | Symbiobacterium thermophilum | |
120.3 | - |
meso-2,6-diaminoheptanedioate | wild-type, pH not specified in the publication, temperature not specified in the publication | Corynebacterium glutamicum | |
279.4 | - |
meso-2,6-diaminoheptanedioate | mutant R71K, pH not specified in the publication, temperature not specified in the publication | Symbiobacterium thermophilum |