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Literature summary for 1.4.1.16 extracted from

  • Akita, H.; Nakamichi, Y.; Morita, T.; Matsushika, A.
    Characterization of an NAD(P)+-dependent meso-diaminopimelate dehydrogenase from Thermosyntropha lipolytica (2020), Biochim. Biophys. Acta, 1868, 140476 .
    View publication on PubMed
Search for more literature for 1.4.1.16

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Thermosyntropha lipolytica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.828
-
 NADP+ pH 10.5, 50°C Thermosyntropha lipolytica
5.68
-
 meso-2,6-diaminoheptanedioate pH 10.5, 50°C Thermosyntropha lipolytica
14.8
-
 NAD+ pH 10.5, 50°C Thermosyntropha lipolytica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
220000
-
 PAGE Thermosyntropha lipolytica

Organism

Organism UniProt Comment Textmining
Thermosyntropha lipolytica A0A1M5M5H3
-
-
Thermosyntropha lipolytica DSM 11003 A0A1M5M5H3
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Thermosyntropha lipolytica

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
31.8
-
 pH 10.5, 50°C Thermosyntropha lipolytica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxobutanoate + NH3 + NADPH + H+
-
 Thermosyntropha lipolytica D-2-aminobutanoate + NADP+
-
 ?
2-oxobutanoate + NH3 + NADPH + H+
-
 Thermosyntropha lipolytica DSM 11003 D-2-aminobutanoate + NADP+
-
 ?
2-oxoglutarate + NH3 + NADPH + H+
-
 Thermosyntropha lipolytica D-glutamate + NADP+
-
 ?
2-oxoglutarate + NH3 + NADPH + H+
-
 Thermosyntropha lipolytica DSM 11003 D-glutamate + NADP+
-
 ?
2-oxooctanoate + NH3 + NADPH + H+
-
 Thermosyntropha lipolytica D-2-aminooctanoate + NADP+
-
 ?
2-oxooctanoate + NH3 + NADPH + H+
-
 Thermosyntropha lipolytica DSM 11003 D-2-aminooctanoate + NADP+
-
 ?
3-methyl-2-oxobutanoate + NH3 + NADPH + H+
-
 Thermosyntropha lipolytica D-valine + NADP+
-
 ?
3-methyl-2-oxopentanoic acid + NH3 + NADPH + H+
-
 Thermosyntropha lipolytica D-isoleucine + NADP+
-
 ?
4-methyl-2-oxopentanoate + NH3 + NADPH + H+
-
 Thermosyntropha lipolytica D-leucine + NADP+
-
 ?
4-methylthio-2-oxobutanoate + NH3 + NADPH + H+
-
 Thermosyntropha lipolytica D-methionine + NADP+
-
 ?
meso-2,6-diaminoheptanedioate + H2O + NAD+
-
 Thermosyntropha lipolytica L-2-amino-6-oxoheptanedioate + NH3 + NADH + H+
-
 ?
meso-2,6-diaminoheptanedioate + H2O + NAD+
-
 Thermosyntropha lipolytica DSM 11003 L-2-amino-6-oxoheptanedioate + NH3 + NADH + H+
-
 ?
oxaloacetate + NH3 + NADPH + H+
-
 Thermosyntropha lipolytica D-aspartate + NADP+
-
 ?
pyruvate + NH3 + NADPH + H+
-
 Thermosyntropha lipolytica D-alanine + NADP+
-
 ?
pyruvate + NH3 + NADPH + H+
-
 Thermosyntropha lipolytica DSM 11003 D-alanine + NADP+
-
 ?

Subunits

Subunits Comment Organism
hexamer 6 * 35000, SDS-PAGE Thermosyntropha lipolytica

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
737
-
 meso-2,6-diaminoheptanedioate pH 10.5, 50°C Thermosyntropha lipolytica
775
-
 NAD+ pH 10.5, 50°C Thermosyntropha lipolytica
830
-
 NADP+ pH 10.5, 50°C Thermosyntropha lipolytica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
 optimum for reductive amination, 50°C Thermosyntropha lipolytica
10.5
-
 optimum for oxidative deamination, 50°C Thermosyntropha lipolytica

Cofactor

Cofactor Comment Organism Structure
NAD+ NADP+ is 20fold preferred over NAD+ Thermosyntropha lipolytica
NADP+ NADP+ is 20fold preferred over NAD+ Thermosyntropha lipolytica

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5.22
-
 NAD+ pH 10.5, 50°C Thermosyntropha lipolytica
13
-
 meso-2,6-diaminoheptanedioate pH 10.5, 50°C Thermosyntropha lipolytica
100.2
-
 NADP+ pH 10.5, 50°C Thermosyntropha lipolytica