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Literature summary for 1.4.1.16 extracted from

  • Stone, V.N.; Parikh, H.I.; El-rami, F.; Ge, X.; Chen, W.; Zhang, Y.; Kellogg, G.E.; Xu, P.
    Identification of small-molecule inhibitors against meso-2,6-diaminopimelate dehydrogenase from Porphyromonas gingivalis (2015), PLoS ONE, 10, e0141126 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.4.1.16

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) pLysS cells Porphyromonas gingivalis

Inhibitors

Inhibitors Comment Organism Structure
(2E,6S)-6-aminohept-2-enedioic acid
-
 Porphyromonas gingivalis
(2S)-2-amino-6-methylideneheptanedioic acid
-
 Porphyromonas gingivalis
(3S)-3-amino-4-{[(1S)-1-carboxy-2-phenylethyl]amino}-4-oxobutanoic acid 2% inhibition at 3 mM Porphyromonas gingivalis
(5S)-5-[(2S)-2-amino-2-carboxyethyl]-4,5-dihydro-1,2-oxazole-3-carboxylic acid
-
 Porphyromonas gingivalis
2-[(3S,4S)-3-carboxy-4-propylpyrrolidin-1-yl]-6-methylpyridine-3-carboxylic acid
-
 Porphyromonas gingivalis
3-([3-[(4-methoxybenzene-1-sulfonyl)amino]quinoxalin-2-yl]amino)benzoic acid
-
 Porphyromonas gingivalis
3-([4-[(3,4-dimethylbenzene-1-sulfonyl)amino]-1-hydroxynaphthalen-2-yl]sulfanyl)propanoic acid
-
 Porphyromonas gingivalis
4-oxo-4-[4-[(quinoxalin-2-yl)sulfamoyl]anilino]butanoic acid
-
 Porphyromonas gingivalis
5-carboxy-2,2-dihydroxypentanoate 13% inhibition at 3 mM Porphyromonas gingivalis
5-[(3-carboxyphenyl)sulfamoyl]-2-hydroxybenzoic acid 6% inhibition at 3 mM Porphyromonas gingivalis
N-[3-[(benzenesulfonyl)amino]phenyl]-3,5-dimethyl-1H-pyrazole-4-sulfonamide 17% inhibition at 3 mM Porphyromonas gingivalis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.06
-
 NADP+ at pH 10.5 and 25°C Porphyromonas gingivalis
0.37
-
 meso-2,6-diaminoheptanedioate at pH 10.5 and 25°C Porphyromonas gingivalis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
meso-2,6-diaminoheptanedioate + H2O + NADP+ Porphyromonas gingivalis
-
 L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+
-
 ?
meso-2,6-diaminoheptanedioate + H2O + NADP+ Porphyromonas gingivalis W83
-
 L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Porphyromonas gingivalis Q7MW40
-
-
Porphyromonas gingivalis W83 Q7MW40
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Porphyromonas gingivalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
meso-2,6-diaminoheptanedioate + H2O + NADP+
-
 Porphyromonas gingivalis L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+
-
 ?
meso-2,6-diaminoheptanedioate + H2O + NADP+
-
 Porphyromonas gingivalis W83 L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+
-
 ?

Subunits

Subunits Comment Organism
? x * 32000, SDS-PAGE Porphyromonas gingivalis

Synonyms

Synonyms Comment Organism
DDH
-
 Porphyromonas gingivalis
meso-2,6-diaminopimelate dehydrogenase
-
 Porphyromonas gingivalis

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Porphyromonas gingivalis

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.157
-
 at pH 10.5 and 25°C Porphyromonas gingivalis 3-([3-[(4-methoxybenzene-1-sulfonyl)amino]quinoxalin-2-yl]amino)benzoic acid
0.31
-
 at pH 10.5 and 25°C Porphyromonas gingivalis 3-([4-[(3,4-dimethylbenzene-1-sulfonyl)amino]-1-hydroxynaphthalen-2-yl]sulfanyl)propanoic acid
0.356
-
 at pH 10.5 and 25°C Porphyromonas gingivalis 4-oxo-4-[4-[(quinoxalin-2-yl)sulfamoyl]anilino]butanoic acid
1.164
-
 at pH 10.5 and 25°C Porphyromonas gingivalis 2-[(3S,4S)-3-carboxy-4-propylpyrrolidin-1-yl]-6-methylpyridine-3-carboxylic acid