Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Symbiobacterium thermophilum |
Crystallization (Comment) | Organism |
---|---|
apo form and in complex with NADP+ and both NADPH and meso-2,6-diaminoheptanedioate, sitting drop vapor diffusion method, using 0.1m HEPES buffer (pH 7.5) with 30% 2-methyl-2,4-pentanediol and 5% (v/v) glycerol | Symbiobacterium thermophilum |
Protein Variants | Comment | Organism |
---|---|---|
M152A | the mutant shows increased activity towards meso-2,6-diaminoheptanedioate and reduced activity towards pyruvate compared to the wild type enzyme | Symbiobacterium thermophilum |
M152D | inactive | Symbiobacterium thermophilum |
M152E | inactive | Symbiobacterium thermophilum |
M152H | the mutant shows increased activity towards meso-2,6-diaminoheptanedioate and no activity towards pyruvate compared to the wild type enzyme | Symbiobacterium thermophilum |
M152K | the mutant shows increased activity towards meso-2,6-diaminoheptanedioate and reduced activity towards pyruvate compared to the wild type enzyme | Symbiobacterium thermophilum |
M152L | the mutant shows increased activity towards meso-2,6-diaminoheptanedioate and pyruvate compared to the wild type enzyme | Symbiobacterium thermophilum |
M152N | the mutant shows increased activity towards meso-2,6-diaminoheptanedioate and reduced activity towards pyruvate compared to the wild type enzyme | Symbiobacterium thermophilum |
M152S | the mutant shows increased activity towards meso-2,6-diaminoheptanedioate and reduced activity towards pyruvate compared to the wild type enzyme | Symbiobacterium thermophilum |
M152W | inactive | Symbiobacterium thermophilum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
meso-2,6-diaminoheptanedioate + H2O + NADP+ | Symbiobacterium thermophilum | - |
L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Symbiobacterium thermophilum | Q67PI3 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography and Superdex 200 gel filtration | Symbiobacterium thermophilum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
meso-2,6-diaminoheptanedioate + H2O + NADP+ | - |
Symbiobacterium thermophilum | L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+ | - |
? | |
meso-2,6-diaminoheptanedioate + H2O + NADP+ | - |
Symbiobacterium thermophilum | L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+ | - |
r | |
pyruvate + NADPH | - |
Symbiobacterium thermophilum | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DAPDH | - |
Symbiobacterium thermophilum |
meso-diaminopimelate dehydrogenase | - |
Symbiobacterium thermophilum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Symbiobacterium thermophilum |