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Literature summary for 1.4.1.16 extracted from

  • Liu, W.; Li, Z.; Huang, C.H.; Guo, R.T.; Zhao, L.; Zhang, D.; Chen, X.; Wu, Q.; Zhu, D.
    Structural and mutational studies on the unusual substrate specificity of meso-diaminopimelate dehydrogenase from Symbiobacterium thermophilum (2014), ChemBioChem, 15, 217-222 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Symbiobacterium thermophilum

Crystallization (Commentary)

Crystallization (Comment) Organism
apo form and in complex with NADP+ and both NADPH and meso-2,6-diaminoheptanedioate, sitting drop vapor diffusion method, using 0.1m HEPES buffer (pH 7.5) with 30% 2-methyl-2,4-pentanediol and 5% (v/v) glycerol Symbiobacterium thermophilum

Protein Variants

Protein Variants Comment Organism
M152A the mutant shows increased activity towards meso-2,6-diaminoheptanedioate and reduced activity towards pyruvate compared to the wild type enzyme Symbiobacterium thermophilum
M152D inactive Symbiobacterium thermophilum
M152E inactive Symbiobacterium thermophilum
M152H the mutant shows increased activity towards meso-2,6-diaminoheptanedioate and no activity towards pyruvate compared to the wild type enzyme Symbiobacterium thermophilum
M152K the mutant shows increased activity towards meso-2,6-diaminoheptanedioate and reduced activity towards pyruvate compared to the wild type enzyme Symbiobacterium thermophilum
M152L the mutant shows increased activity towards meso-2,6-diaminoheptanedioate and pyruvate compared to the wild type enzyme Symbiobacterium thermophilum
M152N the mutant shows increased activity towards meso-2,6-diaminoheptanedioate and reduced activity towards pyruvate compared to the wild type enzyme Symbiobacterium thermophilum
M152S the mutant shows increased activity towards meso-2,6-diaminoheptanedioate and reduced activity towards pyruvate compared to the wild type enzyme Symbiobacterium thermophilum
M152W inactive Symbiobacterium thermophilum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
meso-2,6-diaminoheptanedioate + H2O + NADP+ Symbiobacterium thermophilum
-
L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+
-
r

Organism

Organism UniProt Comment Textmining
Symbiobacterium thermophilum Q67PI3
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and Superdex 200 gel filtration Symbiobacterium thermophilum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
meso-2,6-diaminoheptanedioate + H2O + NADP+
-
Symbiobacterium thermophilum L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+
-
?
meso-2,6-diaminoheptanedioate + H2O + NADP+
-
Symbiobacterium thermophilum L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+
-
r
pyruvate + NADPH
-
Symbiobacterium thermophilum ?
-
?

Synonyms

Synonyms Comment Organism
DAPDH
-
Symbiobacterium thermophilum
meso-diaminopimelate dehydrogenase
-
Symbiobacterium thermophilum

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Symbiobacterium thermophilum