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Literature summary for 1.4.1.16 extracted from

  • Gao, X.; Zhang, Z.; Zhang, Y.; Li, Y.; Zhu, H.; Wang, S.; Li, C.
    A newly determined member of the meso-diaminopimelate dehydrogenase family with a broad substrate spectrum (2017), Appl. Environ. Microbiol., 83, e00476 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.4.1.16

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Symbiobacterium thermophilum

Protein Variants

Protein Variants Comment Organism
R35S the mutant shows reduced catalytic efficiency with meso-2,6-diaminoheptanedioate and NADP+ compared to the wild type enzyme Symbiobacterium thermophilum
R71A the mutant shows about wild type catalytic efficiency with meso-2,6-diaminoheptanedioate and increased catalytic efficiency with NADP+ compared to the wild type enzyme Symbiobacterium thermophilum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
 NADPH wild type enzyme, at pH 9.6 and 25°C Symbiobacterium thermophilum
0.18
-
 NADPH mutant enzyme R71A, at pH 9.6 and 25°C Symbiobacterium thermophilum
0.35
-
 NADPH mutant enzyme R35S, at pH 9.6 and 25°C Symbiobacterium thermophilum
0.35
-
 NADP+ wild type enzyme, at pH 9.6 and 25°C Symbiobacterium thermophilum
0.39
-
 meso-2,6-diaminoheptanedioate mutant enzyme R35S, at pH 9.6 and 25°C Symbiobacterium thermophilum
0.39
-
 NADP+ mutant enzyme R71A, at pH 9.6 and 25°C Symbiobacterium thermophilum
0.41
-
 meso-2,6-diaminoheptanedioate wild type enzyme, at pH 9.6 and 25°C Symbiobacterium thermophilum
0.42
-
 meso-2,6-diaminoheptanedioate mutant enzyme R71A, at pH 9.6 and 25°C Symbiobacterium thermophilum
0.46
-
 NADP+ mutant enzyme R35S, at pH 9.6 and 25°C Symbiobacterium thermophilum
7.45
-
 pyruvate mutant enzyme R35S, at pH 8.6 and 25°C Symbiobacterium thermophilum
16.31
-
 pyruvate wild type enzyme, at pH 8.6 and 25°C Symbiobacterium thermophilum
21.77
-
 pyruvate mutant enzyme R71A, at pH 8.6 and 25°C Symbiobacterium thermophilum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
meso-2,6-diaminoheptanedioate + H2O + NADP+ Symbiobacterium thermophilum
-
 L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+
-
 r
meso-2,6-diaminoheptanedioate + H2O + NADP+ Symbiobacterium thermophilum IAM14863
-
 L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+
-
 r

Organism

Organism UniProt Comment Textmining
Symbiobacterium thermophilum
-
-
-
Symbiobacterium thermophilum IAM14863
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxosuccinic acid + NH3 + NADPH + H+ low activity Symbiobacterium thermophilum ?
-
 ?
2-oxosuccinic acid + NH3 + NADPH + H+ low activity Symbiobacterium thermophilum IAM14863 ?
-
 ?
3-methyl-2-oxobutanoate + NH3 + NADPH + H+ low activity Symbiobacterium thermophilum ?
-
 ?
3-methyl-2-oxobutanoate + NH3 + NADPH + H+ low activity Symbiobacterium thermophilum IAM14863 ?
-
 ?
4-methyl-2-oxopentanoic acid + NH3 + NADPH + H+ low activity Symbiobacterium thermophilum ?
-
 ?
meso-2,6-diaminoheptanedioate + H2O + NADP+
-
 Symbiobacterium thermophilum L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+
-
 r
meso-2,6-diaminoheptanedioate + H2O + NADP+ highest activity Symbiobacterium thermophilum L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+
-
 r
meso-2,6-diaminoheptanedioate + H2O + NADP+
-
 Symbiobacterium thermophilum IAM14863 L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+
-
 r
meso-2,6-diaminoheptanedioate + H2O + NADP+ highest activity Symbiobacterium thermophilum IAM14863 L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+
-
 r
pyruvate + NADPH
-
 Symbiobacterium thermophilum ?
-
 ?
pyruvate + NADPH
-
 Symbiobacterium thermophilum IAM14863 ?
-
 ?

Synonyms

Synonyms Comment Organism
meso-DAPDH
-
 Symbiobacterium thermophilum
meso-diaminopimelate dehydrogenase
-
 Symbiobacterium thermophilum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.92
-
 NADPH mutant enzyme R35S, at pH 9.6 and 25°C Symbiobacterium thermophilum
2.96
-
 pyruvate mutant enzyme R35S, at pH 8.6 and 25°C Symbiobacterium thermophilum
3.31
-
 pyruvate mutant enzyme R71A, at pH 8.6 and 25°C Symbiobacterium thermophilum
4.17
-
 NADPH mutant enzyme R71A, at pH 9.6 and 25°C Symbiobacterium thermophilum
6.97
-
 NADPH wild type enzyme, at pH 9.6 and 25°C Symbiobacterium thermophilum
7
-
 pyruvate wild type enzyme, at pH 8.6 and 25°C Symbiobacterium thermophilum
17.91
-
 NADP+ mutant enzyme R35S, at pH 9.6 and 25°C Symbiobacterium thermophilum
25.47
-
 NADP+ wild type enzyme, at pH 9.6 and 25°C Symbiobacterium thermophilum
29.44
-
 meso-2,6-diaminoheptanedioate mutant enzyme R35S, at pH 9.6 and 25°C Symbiobacterium thermophilum
40.46
-
 meso-2,6-diaminoheptanedioate mutant enzyme R71A, at pH 9.6 and 25°C Symbiobacterium thermophilum
40.47
-
 meso-2,6-diaminoheptanedioate wild type enzyme, at pH 9.6 and 25°C Symbiobacterium thermophilum
58.16
-
 NADP+ mutant enzyme R71A, at pH 9.6 and 25°C Symbiobacterium thermophilum

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Symbiobacterium thermophilum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.15
-
 pyruvate mutant enzyme R71A, at pH 8.6 and 25°C Symbiobacterium thermophilum
0.4
-
 pyruvate mutant enzyme R35S, at pH 8.6 and 25°C Symbiobacterium thermophilum
0.43
-
 pyruvate wild type enzyme, at pH 8.6 and 25°C Symbiobacterium thermophilum
8.32
-
 NADPH mutant enzyme R35S, at pH 9.6 and 25°C Symbiobacterium thermophilum
23.49
-
 NADPH mutant enzyme R71A, at pH 9.6 and 25°C Symbiobacterium thermophilum
39.02
-
 NADP+ mutant enzyme R35S, at pH 9.6 and 25°C Symbiobacterium thermophilum
69.29
-
 NADPH wild type enzyme, at pH 9.6 and 25°C Symbiobacterium thermophilum
73.83
-
 NADP+ wild type enzyme, at pH 9.6 and 25°C Symbiobacterium thermophilum
75.07
-
 meso-2,6-diaminoheptanedioate mutant enzyme R35S, at pH 9.6 and 25°C Symbiobacterium thermophilum
96.54
-
 meso-2,6-diaminoheptanedioate mutant enzyme R71A, at pH 9.6 and 25°C Symbiobacterium thermophilum
98.48
-
 meso-2,6-diaminoheptanedioate wild type enzyme, at pH 9.6 and 25°C Symbiobacterium thermophilum
148.06
-
 NADP+ mutant enzyme R71A, at pH 9.6 and 25°C Symbiobacterium thermophilum