BRENDA - Enzyme Database show
show all sequences of 1.4.1.16

A newly determined member of the meso-diaminopimelate dehydrogenase family with a broad substrate spectrum

Gao, X.; Zhang, Z.; Zhang, Y.; Li, Y.; Zhu, H.; Wang, S.; Li, C.; Appl. Environ. Microbiol. 83, e00476 (2017)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Symbiobacterium thermophilum
Engineering
Amino acid exchange
Commentary
Organism
R35S
the mutant shows reduced catalytic efficiency with meso-2,6-diaminoheptanedioate and NADP+ compared to the wild type enzyme
Symbiobacterium thermophilum
R71A
the mutant shows about wild type catalytic efficiency with meso-2,6-diaminoheptanedioate and increased catalytic efficiency with NADP+ compared to the wild type enzyme
Symbiobacterium thermophilum
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.1
-
NADPH
wild type enzyme, at pH 9.6 and 25°C
Symbiobacterium thermophilum
0.18
-
NADPH
mutant enzyme R71A, at pH 9.6 and 25°C
Symbiobacterium thermophilum
0.35
-
NADP+
wild type enzyme, at pH 9.6 and 25°C
Symbiobacterium thermophilum
0.35
-
NADPH
mutant enzyme R35S, at pH 9.6 and 25°C
Symbiobacterium thermophilum
0.39
-
meso-2,6-diaminoheptanedioate
mutant enzyme R35S, at pH 9.6 and 25°C
Symbiobacterium thermophilum
0.39
-
NADP+
mutant enzyme R71A, at pH 9.6 and 25°C
Symbiobacterium thermophilum
0.41
-
meso-2,6-diaminoheptanedioate
wild type enzyme, at pH 9.6 and 25°C
Symbiobacterium thermophilum
0.42
-
meso-2,6-diaminoheptanedioate
mutant enzyme R71A, at pH 9.6 and 25°C
Symbiobacterium thermophilum
0.46
-
NADP+
mutant enzyme R35S, at pH 9.6 and 25°C
Symbiobacterium thermophilum
7.45
-
pyruvate
mutant enzyme R35S, at pH 8.6 and 25°C
Symbiobacterium thermophilum
16.31
-
pyruvate
wild type enzyme, at pH 8.6 and 25°C
Symbiobacterium thermophilum
21.77
-
pyruvate
mutant enzyme R71A, at pH 8.6 and 25°C
Symbiobacterium thermophilum
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
meso-2,6-diaminoheptanedioate + H2O + NADP+
Symbiobacterium thermophilum
-
L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+
-
-
r
meso-2,6-diaminoheptanedioate + H2O + NADP+
Symbiobacterium thermophilum IAM14863
-
L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+
-
-
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Symbiobacterium thermophilum
-
-
-
Symbiobacterium thermophilum IAM14863
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-oxosuccinic acid + NH3 + NADPH + H+
low activity
741705
Symbiobacterium thermophilum
?
-
-
-
?
2-oxosuccinic acid + NH3 + NADPH + H+
low activity
741705
Symbiobacterium thermophilum IAM14863
?
-
-
-
?
3-methyl-2-oxobutanoate + NH3 + NADPH + H+
low activity
741705
Symbiobacterium thermophilum
?
-
-
-
?
3-methyl-2-oxobutanoate + NH3 + NADPH + H+
low activity
741705
Symbiobacterium thermophilum IAM14863
?
-
-
-
?
4-methyl-2-oxopentanoic acid + NH3 + NADPH + H+
low activity
741705
Symbiobacterium thermophilum
?
-
-
-
?
meso-2,6-diaminoheptanedioate + H2O + NADP+
-
741705
Symbiobacterium thermophilum
L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+
-
-
-
r
meso-2,6-diaminoheptanedioate + H2O + NADP+
highest activity
741705
Symbiobacterium thermophilum
L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+
-
-
-
r
meso-2,6-diaminoheptanedioate + H2O + NADP+
-
741705
Symbiobacterium thermophilum IAM14863
L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+
-
-
-
r
meso-2,6-diaminoheptanedioate + H2O + NADP+
highest activity
741705
Symbiobacterium thermophilum IAM14863
L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+
-
-
-
r
pyruvate + NADPH
-
741705
Symbiobacterium thermophilum
?
-
-
-
?
pyruvate + NADPH
-
741705
Symbiobacterium thermophilum IAM14863
?
-
-
-
?
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.92
-
NADPH
mutant enzyme R35S, at pH 9.6 and 25°C
Symbiobacterium thermophilum
2.96
-
pyruvate
mutant enzyme R35S, at pH 8.6 and 25°C
Symbiobacterium thermophilum
3.31
-
pyruvate
mutant enzyme R71A, at pH 8.6 and 25°C
Symbiobacterium thermophilum
4.17
-
NADPH
mutant enzyme R71A, at pH 9.6 and 25°C
Symbiobacterium thermophilum
6.97
-
NADPH
wild type enzyme, at pH 9.6 and 25°C
Symbiobacterium thermophilum
7
-
pyruvate
wild type enzyme, at pH 8.6 and 25°C
Symbiobacterium thermophilum
17.91
-
NADP+
mutant enzyme R35S, at pH 9.6 and 25°C
Symbiobacterium thermophilum
25.47
-
NADP+
wild type enzyme, at pH 9.6 and 25°C
Symbiobacterium thermophilum
29.44
-
meso-2,6-diaminoheptanedioate
mutant enzyme R35S, at pH 9.6 and 25°C
Symbiobacterium thermophilum
40.46
-
meso-2,6-diaminoheptanedioate
mutant enzyme R71A, at pH 9.6 and 25°C
Symbiobacterium thermophilum
40.47
-
meso-2,6-diaminoheptanedioate
wild type enzyme, at pH 9.6 and 25°C
Symbiobacterium thermophilum
58.16
-
NADP+
mutant enzyme R71A, at pH 9.6 and 25°C
Symbiobacterium thermophilum
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
-
Symbiobacterium thermophilum
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Symbiobacterium thermophilum
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
-
Symbiobacterium thermophilum
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
R35S
the mutant shows reduced catalytic efficiency with meso-2,6-diaminoheptanedioate and NADP+ compared to the wild type enzyme
Symbiobacterium thermophilum
R71A
the mutant shows about wild type catalytic efficiency with meso-2,6-diaminoheptanedioate and increased catalytic efficiency with NADP+ compared to the wild type enzyme
Symbiobacterium thermophilum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.1
-
NADPH
wild type enzyme, at pH 9.6 and 25°C
Symbiobacterium thermophilum
0.18
-
NADPH
mutant enzyme R71A, at pH 9.6 and 25°C
Symbiobacterium thermophilum
0.35
-
NADP+
wild type enzyme, at pH 9.6 and 25°C
Symbiobacterium thermophilum
0.35
-
NADPH
mutant enzyme R35S, at pH 9.6 and 25°C
Symbiobacterium thermophilum
0.39
-
meso-2,6-diaminoheptanedioate
mutant enzyme R35S, at pH 9.6 and 25°C
Symbiobacterium thermophilum
0.39
-
NADP+
mutant enzyme R71A, at pH 9.6 and 25°C
Symbiobacterium thermophilum
0.41
-
meso-2,6-diaminoheptanedioate
wild type enzyme, at pH 9.6 and 25°C
Symbiobacterium thermophilum
0.42
-
meso-2,6-diaminoheptanedioate
mutant enzyme R71A, at pH 9.6 and 25°C
Symbiobacterium thermophilum
0.46
-
NADP+
mutant enzyme R35S, at pH 9.6 and 25°C
Symbiobacterium thermophilum
7.45
-
pyruvate
mutant enzyme R35S, at pH 8.6 and 25°C
Symbiobacterium thermophilum
16.31
-
pyruvate
wild type enzyme, at pH 8.6 and 25°C
Symbiobacterium thermophilum
21.77
-
pyruvate
mutant enzyme R71A, at pH 8.6 and 25°C
Symbiobacterium thermophilum
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
meso-2,6-diaminoheptanedioate + H2O + NADP+
Symbiobacterium thermophilum
-
L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+
-
-
r
meso-2,6-diaminoheptanedioate + H2O + NADP+
Symbiobacterium thermophilum IAM14863
-
L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+
-
-
r
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-oxosuccinic acid + NH3 + NADPH + H+
low activity
741705
Symbiobacterium thermophilum
?
-
-
-
?
2-oxosuccinic acid + NH3 + NADPH + H+
low activity
741705
Symbiobacterium thermophilum IAM14863
?
-
-
-
?
3-methyl-2-oxobutanoate + NH3 + NADPH + H+
low activity
741705
Symbiobacterium thermophilum
?
-
-
-
?
3-methyl-2-oxobutanoate + NH3 + NADPH + H+
low activity
741705
Symbiobacterium thermophilum IAM14863
?
-
-
-
?
4-methyl-2-oxopentanoic acid + NH3 + NADPH + H+
low activity
741705
Symbiobacterium thermophilum
?
-
-
-
?
meso-2,6-diaminoheptanedioate + H2O + NADP+
-
741705
Symbiobacterium thermophilum
L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+
-
-
-
r
meso-2,6-diaminoheptanedioate + H2O + NADP+
highest activity
741705
Symbiobacterium thermophilum
L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+
-
-
-
r
meso-2,6-diaminoheptanedioate + H2O + NADP+
-
741705
Symbiobacterium thermophilum IAM14863
L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+
-
-
-
r
meso-2,6-diaminoheptanedioate + H2O + NADP+
highest activity
741705
Symbiobacterium thermophilum IAM14863
L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+
-
-
-
r
pyruvate + NADPH
-
741705
Symbiobacterium thermophilum
?
-
-
-
?
pyruvate + NADPH
-
741705
Symbiobacterium thermophilum IAM14863
?
-
-
-
?
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.92
-
NADPH
mutant enzyme R35S, at pH 9.6 and 25°C
Symbiobacterium thermophilum
2.96
-
pyruvate
mutant enzyme R35S, at pH 8.6 and 25°C
Symbiobacterium thermophilum
3.31
-
pyruvate
mutant enzyme R71A, at pH 8.6 and 25°C
Symbiobacterium thermophilum
4.17
-
NADPH
mutant enzyme R71A, at pH 9.6 and 25°C
Symbiobacterium thermophilum
6.97
-
NADPH
wild type enzyme, at pH 9.6 and 25°C
Symbiobacterium thermophilum
7
-
pyruvate
wild type enzyme, at pH 8.6 and 25°C
Symbiobacterium thermophilum
17.91
-
NADP+
mutant enzyme R35S, at pH 9.6 and 25°C
Symbiobacterium thermophilum
25.47
-
NADP+
wild type enzyme, at pH 9.6 and 25°C
Symbiobacterium thermophilum
29.44
-
meso-2,6-diaminoheptanedioate
mutant enzyme R35S, at pH 9.6 and 25°C
Symbiobacterium thermophilum
40.46
-
meso-2,6-diaminoheptanedioate
mutant enzyme R71A, at pH 9.6 and 25°C
Symbiobacterium thermophilum
40.47
-
meso-2,6-diaminoheptanedioate
wild type enzyme, at pH 9.6 and 25°C
Symbiobacterium thermophilum
58.16
-
NADP+
mutant enzyme R71A, at pH 9.6 and 25°C
Symbiobacterium thermophilum
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.15
-
pyruvate
mutant enzyme R71A, at pH 8.6 and 25°C
Symbiobacterium thermophilum
0.4
-
pyruvate
mutant enzyme R35S, at pH 8.6 and 25°C
Symbiobacterium thermophilum
0.43
-
pyruvate
wild type enzyme, at pH 8.6 and 25°C
Symbiobacterium thermophilum
8.32
-
NADPH
mutant enzyme R35S, at pH 9.6 and 25°C
Symbiobacterium thermophilum
23.49
-
NADPH
mutant enzyme R71A, at pH 9.6 and 25°C
Symbiobacterium thermophilum
39.02
-
NADP+
mutant enzyme R35S, at pH 9.6 and 25°C
Symbiobacterium thermophilum
69.29
-
NADPH
wild type enzyme, at pH 9.6 and 25°C
Symbiobacterium thermophilum
73.83
-
NADP+
wild type enzyme, at pH 9.6 and 25°C
Symbiobacterium thermophilum
75.07
-
meso-2,6-diaminoheptanedioate
mutant enzyme R35S, at pH 9.6 and 25°C
Symbiobacterium thermophilum
96.54
-
meso-2,6-diaminoheptanedioate
mutant enzyme R71A, at pH 9.6 and 25°C
Symbiobacterium thermophilum
98.48
-
meso-2,6-diaminoheptanedioate
wild type enzyme, at pH 9.6 and 25°C
Symbiobacterium thermophilum
148.06
-
NADP+
mutant enzyme R71A, at pH 9.6 and 25°C
Symbiobacterium thermophilum
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.15
-
pyruvate
mutant enzyme R71A, at pH 8.6 and 25°C
Symbiobacterium thermophilum
0.4
-
pyruvate
mutant enzyme R35S, at pH 8.6 and 25°C
Symbiobacterium thermophilum
0.43
-
pyruvate
wild type enzyme, at pH 8.6 and 25°C
Symbiobacterium thermophilum
8.32
-
NADPH
mutant enzyme R35S, at pH 9.6 and 25°C
Symbiobacterium thermophilum
23.49
-
NADPH
mutant enzyme R71A, at pH 9.6 and 25°C
Symbiobacterium thermophilum
39.02
-
NADP+
mutant enzyme R35S, at pH 9.6 and 25°C
Symbiobacterium thermophilum
69.29
-
NADPH
wild type enzyme, at pH 9.6 and 25°C
Symbiobacterium thermophilum
73.83
-
NADP+
wild type enzyme, at pH 9.6 and 25°C
Symbiobacterium thermophilum
75.07
-
meso-2,6-diaminoheptanedioate
mutant enzyme R35S, at pH 9.6 and 25°C
Symbiobacterium thermophilum
96.54
-
meso-2,6-diaminoheptanedioate
mutant enzyme R71A, at pH 9.6 and 25°C
Symbiobacterium thermophilum
98.48
-
meso-2,6-diaminoheptanedioate
wild type enzyme, at pH 9.6 and 25°C
Symbiobacterium thermophilum
148.06
-
NADP+
mutant enzyme R71A, at pH 9.6 and 25°C
Symbiobacterium thermophilum
Other publictions for EC 1.4.1.16
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
741705
Gao
A newly determined member of ...
Symbiobacterium thermophilum, Symbiobacterium thermophilum IAM14863
Appl. Environ. Microbiol.
83
e00476
2017
-
-
1
-
2
-
-
12
-
-
-
2
-
3
-
-
-
-
-
-
-
-
11
-
-
-
-
12
-
-
-
1
-
-
-
-
-
1
1
-
2
-
-
-
-
12
-
-
-
2
-
-
-
-
-
-
-
-
11
-
-
-
-
12
-
-
-
-
-
-
-
-
12
12
741514
Akita
Structural insight into the t ...
Ureibacillus thermosphaericus
Acta Crystallogr. Sect. D
71
1136-1146
2015
-
-
1
1
-
-
-
-
-
-
-
1
-
4
-
-
1
-
-
-
-
-
1
1
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1
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1
1
1
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1
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1
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1
1
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-
-
-
-
-
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-
742282
Liu
Structural analysis reveals t ...
Clostridium tetani, Clostridium tetani E88
ChemBioChem
16
924-929
2015
-
-
1
1
1
-
-
-
-
-
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2
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3
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1
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3
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8
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1
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1
1
1
1
-
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2
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1
-
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3
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
742303
Zhao
Effect of residue Y76 on co-e ...
Symbiobacterium thermophilum
Chin. J. Biotechnol.
31
1108-1118
2015
-
-
-
-
1
-
-
-
-
-
-
1
-
3
-
-
-
-
-
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1
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1
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1
-
1
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-
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1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
743593
Stone
Identification of small-molec ...
Porphyromonas gingivalis, Porphyromonas gingivalis W83
PLoS ONE
10
e0141126
2015
-
-
1
-
-
-
11
2
-
-
-
2
-
5
-
-
1
-
-
-
-
-
2
1
-
-
-
-
-
-
-
1
-
-
4
-
-
1
1
-
-
-
4
11
-
2
-
-
-
2
-
-
-
1
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
742276
Liu
Structural and mutational stu ...
Symbiobacterium thermophilum
ChemBioChem
15
217-222
2014
-
-
1
1
9
-
-
-
-
-
-
1
-
2
-
-
1
-
-
-
-
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
1
9
-
-
-
-
-
-
-
-
1
-
-
-
1
-
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-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
724034
Gao
Engineering the meso-diaminopi ...
Symbiobacterium thermophilum
Appl. Environ. Microbiol.
79
5078-5081
2013
-
-
1
-
10
-
-
9
-
-
-
1
-
2
-
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1
-
-
-
10
-
2
-
1
-
-
10
1
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-
2
-
-
-
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-
1
2
-
10
-
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-
-
9
-
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1
-
-
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1
-
-
10
-
2
-
1
-
-
10
1
-
-
-
-
1
1
-
-
-
724026
Gao
A novel meso-Diaminopimelate d ...
Symbiobacterium thermophilum
Appl. Environ. Microbiol.
78
8595-8600
2012
-
1
1
-
3
-
-
8
-
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2
-
3
-
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1
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4
-
1
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4
8
2
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2
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1
1
2
-
3
-
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8
-
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2
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1
-
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-
-
4
-
1
-
4
8
2
-
-
-
-
1
1
-
-
-
724643
Akita
Creation of a thermostable NAD ...
Ureibacillus thermosphaericus, Ureibacillus thermosphaericus A1
Biotechnol. Lett.
34
1693-1699
2012
-
-
1
-
1
-
8
1
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1
-
2
-
5
-
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1
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2
-
2
-
1
-
2
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2
-
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1
2
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1
-
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8
-
1
-
1
-
2
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1
-
-
-
-
2
-
2
-
1
-
2
-
-
-
-
1
1
-
-
-
723913
Akita
Highly stable meso-diaminopime ...
Ureibacillus thermosphaericus, Ureibacillus thermosphaericus A1
AMB Express
1
43
2011
-
-
1
-
-
-
6
-
-
-
2
4
-
7
-
-
1
-
-
-
1
-
6
1
1
-
2
-
1
-
1
1
-
-
-
-
-
1
1
-
-
-
-
6
-
-
-
-
2
4
-
-
-
1
-
-
1
-
6
1
1
-
2
-
1
-
1
-
-
-
-
-
-
-
712938
Kind
Systems-wide metabolic pathway ...
Corynebacterium glutamicum
Metab. Eng.
12
341-351
2010
-
-
1
-
1
-
-
-
-
-
-
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4
-
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1
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1
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
391524
Cirilli
The three-dimensional structur ...
Corynebacterium glutamicum, Lysinibacillus sphaericus
Protein Sci.
9
2034-2037
2000
-
-
-
1
-
-
1
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
2
1
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
391522
Wang
Substrate binding and conforma ...
Corynebacterium glutamicum
Protein Sci.
7
293-299
1998
-
-
1
-
-
-
-
2
-
-
1
-
-
4
-
-
1
1
-
-
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
2
-
-
1
-
-
-
-
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
391523
Scapin
Substrate and inhibitor bindin ...
Corynebacterium glutamicum
Biochemistry
37
3278-3285
1998
-
-
1
1
-
-
1
-
-
-
-
-
-
3
-
-
1
1
-
-
-
-
1
-
-
-
-
-
-
-
-
1
2
-
-
-
-
1
1
1
-
-
-
1
2
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
391520
Scapin
Three-dimensional structure of ...
Corynebacterium glutamicum
Biochemistry
35
13540-13551
1996
-
-
1
1
-
-
-
-
-
-
-
-
-
5
-
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1
1
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
391521
Reddy
Expression, purification, and ...
Corynebacterium glutamicum
Proteins Struct. Funct. Genet.
25
514-516
1996
-
-
1
1
-
-
-
-
-
-
1
-
-
2
-
-
1
-
-
-
1
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
1
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
1
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2149
Abbott
-
Synthesis and testing of heter ...
Lysinibacillus sphaericus
J. Am. Chem. Soc.
116
6513-6520
1994
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
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1
-
-
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-
-
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-
1
2
-
-
-
-
-
1
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2153
Chatterjee
Biosynthesis of lysine in plan ...
no activity in Chlamydomonas reinhardtii, no activity in Glycine max, no activity in Nicotiana tabacum, no activity in Zea mays
Plant Mol. Biol.
26
285-290
1994
-
-
-
-
-
-
-
-
-
-
-
-
-
9
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2145
Lam
Analogs of diaminopimelic acid ...
Lysinibacillus sphaericus
J. Biol. Chem.
263
11814-11819
1988
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4124
Yeh
General organization of the ge ...
Corynebacterium glutamicum, Corynebacterium glutamicum AS019
Mol. Gen. Genet.
212
105-111
1988
-
-
1
-
-
-
-
-
-
-
-
2
-
5
-
-
-
-
-
-
1
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
1
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390193
Cremer
Regulation of enzymes of lysin ...
Corynebacterium glutamicum
J. Gen. Microbiol.
134
3221-3229
1988
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
391502
Ishino
-
Cloning and sequencing of the ...
Corynebacterium glutamicum, Corynebacterium glutamicum RRL-5
Agric. Biol. Chem.
52
2903-2909
1988
-
-
1
-
-
-
-
-
-
-
1
2
-
2
-
-
-
-
-
-
2
-
4
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
1
2
-
-
-
-
-
-
2
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
391504
Ishino
Nucleotide sequence of the mes ...
Corynebacterium glutamicum
Nucleic Acids Res.
15
3917
1987
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390194
Bartlett
-
Regulation of the enzymes of l ...
Lysinibacillus sphaericus
J. Gen. Microbiol.
132
3169-3177
1986
-
-
-
-
-
-
3
-
-
-
-
1
-
1
-
-
-
-
-
-
3
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
3
-
-
-
-
-
1
-
-
-
-
-
-
3
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
391505
Misono
-
Characterization of meso-diami ...
Achromobacter polymorph, Achromobacter superficialis, Agrobacterium tumefaciens, Alcaligenes viscolactis, Bacillus amyloliquefaciens, Bacillus cereus, Bacillus licheniformis, Bacterium mycoides, Brevibacillus brevis, Brevibacterium sp., Corynebacterium ammoniagenes, Corynebacterium glutamicum, Corynebacterium pseudodiphtheriticum, Enterobacter cloacae, Flavobacterium suaveolens, Glutamicibacter protophormiae, Hafnia alvei, Kocuria rosea, Lysinibacillus sphaericus, Micrococcus luteus, Morganella morganii, no activity in Escherichia coli, no activity in Klebsiella pneumoniae, no activity in Staphylococcus aureus, Providencia rettgeri, Pseudomonas aeruginosa, Pseudomonas alkanolytica, Pseudomonas chlororaphis subsp. aureofaciens, Pseudomonas cruciviae, Pseudomonas fragi, Pseudomonas putida, Terrabacter tumescens
Agric. Biol. Chem.
50
2729-2734
1986
-
-
-
-
-
-
22
9
-
-
3
8
-
32
-
-
1
-
-
-
1
2
37
3
-
-
2
-
2
-
1
31
2
-
-
-
-
-
31
-
-
-
-
22
2
9
-
-
3
8
-
-
-
1
-
-
1
2
37
3
-
-
2
-
2
-
1
-
-
-
-
-
-
-
391507
Misono
-
Purification and properties of ...
Brevibacterium sp., Brevibacterium sp. ICR 7000
Agric. Biol. Chem.
50
1329-1330
1986
-
-
-
-
-
-
5
8
-
-
2
2
-
2
-
-
1
-
-
-
1
-
4
1
-
-
1
-
2
-
1
2
-
-
-
-
-
-
2
-
-
-
-
5
-
8
-
-
2
2
-
-
-
1
-
-
1
-
4
1
-
-
1
-
2
-
1
-
-
-
-
-
-
-
2139
Bartlett
-
Species of Bacillus that make ...
Lysinibacillus sphaericus, Paenibacillus macerans, Sporosarcina globispora, Sporosarcina pasteurii
J. Gen. Microbiol.
131
2145-2152
1985
-
-
-
-
-
-
-
-
-
-
-
4
-
4
-
-
-
-
-
-
3
-
8
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
3
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
391508
White
-
2,6-Diaminopimelate ...
Lysinibacillus sphaericus
Methods Enzym. Anal. , 3rd Ed. (Bergmeyer, H. U. , ed. )
8
377-383
1985
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
1
2
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
2
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
391510
Ishino
-
Involvement of meso-alpha,epsi ...
Corynebacterium glutamicum
Agric. Biol. Chem.
48
2257-2260
1984
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
391511
White
-
The essential role of diaminop ...
Lysinibacillus sphaericus
J. Gen. Microbiol.
129
739-749
1983
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
2
-
2
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
2
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
391512
Misono
-
meso-alpha,epsilon-Diaminopime ...
Lysinibacillus sphaericus
Agric. Biol. Chem.
45
1455-1460
1981
-
-
-
-
-
-
6
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
6
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
391513
Misono
Properties of meso-alpha,epsil ...
Lysinibacillus sphaericus
J. Biol. Chem.
255
10599-10605
1980
-
-
-
-
-
-
7
6
-
-
2
1
-
1
-
-
1
1
-
-
-
2
2
1
-
-
1
-
2
2
1
1
3
-
-
-
-
-
1
-
-
-
-
7
3
6
-
-
2
1
-
-
-
1
-
-
-
2
2
1
-
-
1
-
2
2
1
-
-
-
-
-
-
-
391514
Misono
-
Purification and properties of ...
Lysinibacillus sphaericus
Agric. Biol. Chem.
44
227-229
1980
-
-
-
-
-
-
-
3
-
-
1
1
-
1
-
-
1
-
-
-
1
-
2
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
3
-
-
1
1
-
-
-
1
-
-
1
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
391515
Misono
meso-alpha,epsilon-Diaminopime ...
Brevibacterium sp., Corynebacterium glutamicum, Erwinia aroidea, Lysinibacillus sphaericus, Proteus mirabilis, Proteus vulgaris, Pseudomonas fluorescens, Sarcina subflava
J. Bacteriol.
137
22-27
1979
-
-
-
-
-
-
-
-
-
-
-
4
-
8
-
-
-
-
-
-
-
-
12
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
12
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
391516
Misono
Occurrence of meso-alpha, epsi ...
Lysinibacillus sphaericus
Biochem. Biophys. Res. Commun.
72
89-93
1976
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
2
-
2
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
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2
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2
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1
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