BRENDA - Enzyme Database show
show all sequences of 1.4.1.16

A novel meso-Diaminopimelate dehydrogenase from Symbiobacterium thermophilum: overexpression, characterization, and potential for D-amino acid synthesis

Gao, X.; Chen, X.; Liu, W.; Feng, J.; Wu, Q.; Hua, L.; Zhu, D.; Appl. Environ. Microbiol. 78, 8595-8600 (2012)

Data extracted from this reference:

Application
Application
Commentary
Organism
biotechnology
high thermostability and relaxed substrate profile of Symbiobacterium thermophilum meso-DAPDH warrant it as an excellent starting enzyme for creating effective D-amino acid dehydrogenases by protein engineering
Symbiobacterium thermophilum
Cloned(Commentary)
Commentary
Organism
DNA and amino acid sequence determmination and analysis, expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3), equally as a soluble protein and in inclusion bodies
Symbiobacterium thermophilum
Engineering
Amino acid exchange
Commentary
Organism
F146W
site-directed mutagenesis, the mutant shows altered activity levels with meso-2,6-diaminoheptanedioate and pyruvate as substrates
Symbiobacterium thermophilum
F146W/M152Q
site-directed mutagenesis, the mutant shows altered activity levels with meso-2,6-diaminoheptanedioate and pyruvate as substrates
Symbiobacterium thermophilum
M152Q
site-directed mutagenesis, the mutant shows altered activity levels with meso-2,6-diaminoheptanedioate and pyruvate as substrates
Symbiobacterium thermophilum
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.9
-
meso-2,6-diaminoheptanedioate
pH 8.5, 30C, recombinant wild-type enzyme
Symbiobacterium thermophilum
2.5
-
meso-2,6-diaminoheptanedioate
pH 8.5, 30C, recombinant mutant F1446W
Symbiobacterium thermophilum
12.2
-
pyruvate
pH 8.5, 30C, recombinant mutant F1446W
Symbiobacterium thermophilum
13.5
-
pyruvate
pH 8.5, 30C, recombinant wild-type enzyme
Symbiobacterium thermophilum
15.7
-
meso-2,6-diaminoheptanedioate
pH 8.5, 30C, recombinant mutant F146W/M152Q
Symbiobacterium thermophilum
16.7
-
meso-2,6-diaminoheptanedioate
pH 8.5, 30C, recombinant mutant M152Q
Symbiobacterium thermophilum
23.6
-
pyruvate
pH 8.5, 30C, recombinant mutant F146W/M152Q
Symbiobacterium thermophilum
24.8
-
pyruvate
pH 8.5, 30C, recombinant mutant M152Q
Symbiobacterium thermophilum
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
meso-2,6-diaminoheptanedioate + H2O + NADP+
Symbiobacterium thermophilum
-
L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+
-
-
r
additional information
Symbiobacterium thermophilum
the enzyme has a more relaxed substrate specificity and potential for D-amino acid synthesis
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Symbiobacterium thermophilum
Q67PI3
-
-
Purification (Commentary)
Commentary
Organism
recombinant soluble enzyme 3.4fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
Symbiobacterium thermophilum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
meso-2,6-diaminoheptanedioate + H2O + NADP+
-
724026
Symbiobacterium thermophilum
L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+
-
-
-
r
additional information
the enzyme has a more relaxed substrate specificity and potential for D-amino acid synthesis
724026
Symbiobacterium thermophilum
?
-
-
-
-
additional information
meso-diaminopimelate dehydrogenase is an NADP-dependent enzyme which catalyzes the reversible oxidative deamination on the D-configuration of meso-2,6-diaminopimelate to produce L-2-amino-6-oxopimelate. The purified enzyme also shows activity toward D-alanine, D-valine, and D-lysine. This enzyme catalyzes the reductive amination of 2-keto acids such as pyruvic acid to generate D-amino acids in up to 99% conversion and 99% enantiomeric excess. Substrate specificities of recombinant wild-type and mutant emzymes in both amination and deamination, overview
724026
Symbiobacterium thermophilum
?
-
-
-
-
pyruvate + NH3 + NADPH + H+
-
724026
Symbiobacterium thermophilum
D-alanine + NADP+
-
-
-
?
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
30
-
assay at
Symbiobacterium thermophilum
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
30
60
purified recombinant enzyme, 30 min, fully stable in deamination activity
Symbiobacterium thermophilum
65
-
purified recombinant enzyme, 30, loss of 50% amination activity
Symbiobacterium thermophilum
70
-
purified recombinant enzyme, 30, loss of 6% deamination activity
Symbiobacterium thermophilum
75
-
purified recombinant enzyme, 1 h, rapid inactivation
Symbiobacterium thermophilum
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
664
-
pyruvate
pH 8.5, 30C, recombinant mutant F146W/M152Q
Symbiobacterium thermophilum
1010
-
pyruvate
pH 8.5, 30C, recombinant mutant M152Q
Symbiobacterium thermophilum
6780
-
meso-2,6-diaminoheptanedioate
pH 8.5, 30C, recombinant mutant F146W/M152Q
Symbiobacterium thermophilum
7850
-
pyruvate
pH 8.5, 30C, recombinant wild-type enzyme
Symbiobacterium thermophilum
10100
-
pyruvate
pH 8.5, 30C, recombinant mutant F1446W
Symbiobacterium thermophilum
15100
-
meso-2,6-diaminoheptanedioate
pH 8.5, 30C, recombinant mutant F1446W
Symbiobacterium thermophilum
35700
-
meso-2,6-diaminoheptanedioate
pH 8.5, 30C, recombinant mutant M152Q
Symbiobacterium thermophilum
127000
-
meso-2,6-diaminoheptanedioate
pH 8.5, 30C, recombinant wild-type enzyme
Symbiobacterium thermophilum
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
amination
Symbiobacterium thermophilum
11.5
-
deamination
Symbiobacterium thermophilum
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
-
Symbiobacterium thermophilum
NADPH
-
Symbiobacterium thermophilum
Application (protein specific)
Application
Commentary
Organism
biotechnology
high thermostability and relaxed substrate profile of Symbiobacterium thermophilum meso-DAPDH warrant it as an excellent starting enzyme for creating effective D-amino acid dehydrogenases by protein engineering
Symbiobacterium thermophilum
Cloned(Commentary) (protein specific)
Commentary
Organism
DNA and amino acid sequence determmination and analysis, expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3), equally as a soluble protein and in inclusion bodies
Symbiobacterium thermophilum
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
-
Symbiobacterium thermophilum
NADPH
-
Symbiobacterium thermophilum
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
F146W
site-directed mutagenesis, the mutant shows altered activity levels with meso-2,6-diaminoheptanedioate and pyruvate as substrates
Symbiobacterium thermophilum
F146W/M152Q
site-directed mutagenesis, the mutant shows altered activity levels with meso-2,6-diaminoheptanedioate and pyruvate as substrates
Symbiobacterium thermophilum
M152Q
site-directed mutagenesis, the mutant shows altered activity levels with meso-2,6-diaminoheptanedioate and pyruvate as substrates
Symbiobacterium thermophilum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.9
-
meso-2,6-diaminoheptanedioate
pH 8.5, 30C, recombinant wild-type enzyme
Symbiobacterium thermophilum
2.5
-
meso-2,6-diaminoheptanedioate
pH 8.5, 30C, recombinant mutant F1446W
Symbiobacterium thermophilum
12.2
-
pyruvate
pH 8.5, 30C, recombinant mutant F1446W
Symbiobacterium thermophilum
13.5
-
pyruvate
pH 8.5, 30C, recombinant wild-type enzyme
Symbiobacterium thermophilum
15.7
-
meso-2,6-diaminoheptanedioate
pH 8.5, 30C, recombinant mutant F146W/M152Q
Symbiobacterium thermophilum
16.7
-
meso-2,6-diaminoheptanedioate
pH 8.5, 30C, recombinant mutant M152Q
Symbiobacterium thermophilum
23.6
-
pyruvate
pH 8.5, 30C, recombinant mutant F146W/M152Q
Symbiobacterium thermophilum
24.8
-
pyruvate
pH 8.5, 30C, recombinant mutant M152Q
Symbiobacterium thermophilum
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
meso-2,6-diaminoheptanedioate + H2O + NADP+
Symbiobacterium thermophilum
-
L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+
-
-
r
additional information
Symbiobacterium thermophilum
the enzyme has a more relaxed substrate specificity and potential for D-amino acid synthesis
?
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant soluble enzyme 3.4fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
Symbiobacterium thermophilum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
meso-2,6-diaminoheptanedioate + H2O + NADP+
-
724026
Symbiobacterium thermophilum
L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+
-
-
-
r
additional information
the enzyme has a more relaxed substrate specificity and potential for D-amino acid synthesis
724026
Symbiobacterium thermophilum
?
-
-
-
-
additional information
meso-diaminopimelate dehydrogenase is an NADP-dependent enzyme which catalyzes the reversible oxidative deamination on the D-configuration of meso-2,6-diaminopimelate to produce L-2-amino-6-oxopimelate. The purified enzyme also shows activity toward D-alanine, D-valine, and D-lysine. This enzyme catalyzes the reductive amination of 2-keto acids such as pyruvic acid to generate D-amino acids in up to 99% conversion and 99% enantiomeric excess. Substrate specificities of recombinant wild-type and mutant emzymes in both amination and deamination, overview
724026
Symbiobacterium thermophilum
?
-
-
-
-
pyruvate + NH3 + NADPH + H+
-
724026
Symbiobacterium thermophilum
D-alanine + NADP+
-
-
-
?
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
30
-
assay at
Symbiobacterium thermophilum
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
30
60
purified recombinant enzyme, 30 min, fully stable in deamination activity
Symbiobacterium thermophilum
65
-
purified recombinant enzyme, 30, loss of 50% amination activity
Symbiobacterium thermophilum
70
-
purified recombinant enzyme, 30, loss of 6% deamination activity
Symbiobacterium thermophilum
75
-
purified recombinant enzyme, 1 h, rapid inactivation
Symbiobacterium thermophilum
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
664
-
pyruvate
pH 8.5, 30C, recombinant mutant F146W/M152Q
Symbiobacterium thermophilum
1010
-
pyruvate
pH 8.5, 30C, recombinant mutant M152Q
Symbiobacterium thermophilum
6780
-
meso-2,6-diaminoheptanedioate
pH 8.5, 30C, recombinant mutant F146W/M152Q
Symbiobacterium thermophilum
7850
-
pyruvate
pH 8.5, 30C, recombinant wild-type enzyme
Symbiobacterium thermophilum
10100
-
pyruvate
pH 8.5, 30C, recombinant mutant F1446W
Symbiobacterium thermophilum
15100
-
meso-2,6-diaminoheptanedioate
pH 8.5, 30C, recombinant mutant F1446W
Symbiobacterium thermophilum
35700
-
meso-2,6-diaminoheptanedioate
pH 8.5, 30C, recombinant mutant M152Q
Symbiobacterium thermophilum
127000
-
meso-2,6-diaminoheptanedioate
pH 8.5, 30C, recombinant wild-type enzyme
Symbiobacterium thermophilum
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
amination
Symbiobacterium thermophilum
11.5
-
deamination
Symbiobacterium thermophilum
General Information
General Information
Commentary
Organism
additional information
amino acid residues F146 and M152 are located in the substrate binding site, but are not critical in determining the enzyme's substrate specificity
Symbiobacterium thermophilum
General Information (protein specific)
General Information
Commentary
Organism
additional information
amino acid residues F146 and M152 are located in the substrate binding site, but are not critical in determining the enzyme's substrate specificity
Symbiobacterium thermophilum
Other publictions for EC 1.4.1.16
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
741705
Gao
A newly determined member of ...
Symbiobacterium thermophilum, Symbiobacterium thermophilum IAM14863
Appl. Environ. Microbiol.
83
e00476
2017
-
-
1
-
2
-
-
12
-
-
-
2
-
3
-
-
-
-
-
-
-
-
11
-
-
-
-
12
-
-
-
1
-
-
-
-
-
1
1
-
2
-
-
-
-
12
-
-
-
2
-
-
-
-
-
-
-
-
11
-
-
-
-
12
-
-
-
-
-
-
-
-
12
12
741514
Akita
Structural insight into the t ...
Ureibacillus thermosphaericus
Acta Crystallogr. Sect. D
71
1136-1146
2015
-
-
1
1
-
-
-
-
-
-
-
1
-
4
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
1
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
742282
Liu
Structural analysis reveals t ...
Clostridium tetani, Clostridium tetani E88
ChemBioChem
16
924-929
2015
-
-
1
1
1
-
-
-
-
-
-
2
-
3
-
-
1
-
-
-
3
-
8
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
1
1
-
-
-
-
-
-
-
-
2
-
-
-
1
-
-
3
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
742303
Zhao
Effect of residue Y76 on co-e ...
Symbiobacterium thermophilum
Chin. J. Biotechnol.
31
1108-1118
2015
-
-
-
-
1
-
-
-
-
-
-
1
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
743593
Stone
Identification of small-molec ...
Porphyromonas gingivalis, Porphyromonas gingivalis W83
PLoS ONE
10
e0141126
2015
-
-
1
-
-
-
11
2
-
-
-
2
-
5
-
-
1
-
-
-
-
-
2
1
-
-
-
-
-
-
-
1
-
-
4
-
-
1
1
-
-
-
4
11
-
2
-
-
-
2
-
-
-
1
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
742276
Liu
Structural and mutational stu ...
Symbiobacterium thermophilum
ChemBioChem
15
217-222
2014
-
-
1
1
9
-
-
-
-
-
-
1
-
2
-
-
1
-
-
-
-
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
1
9
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
724034
Gao
Engineering the meso-diaminopi ...
Symbiobacterium thermophilum
Appl. Environ. Microbiol.
79
5078-5081
2013
-
-
1
-
10
-
-
9
-
-
-
1
-
2
-
-
1
-
-
-
10
-
2
-
1
-
-
10
1
-
-
2
-
-
-
-
-
1
2
-
10
-
-
-
-
9
-
-
-
1
-
-
-
1
-
-
10
-
2
-
1
-
-
10
1
-
-
-
-
1
1
-
-
-
724026
Gao
A novel meso-Diaminopimelate d ...
Symbiobacterium thermophilum
Appl. Environ. Microbiol.
78
8595-8600
2012
-
1
1
-
3
-
-
8
-
-
-
2
-
3
-
-
1
-
-
-
-
-
4
-
1
-
4
8
2
-
-
2
-
-
-
-
1
1
2
-
3
-
-
-
-
8
-
-
-
2
-
-
-
1
-
-
-
-
4
-
1
-
4
8
2
-
-
-
-
1
1
-
-
-
724643
Akita
Creation of a thermostable NAD ...
Ureibacillus thermosphaericus, Ureibacillus thermosphaericus A1
Biotechnol. Lett.
34
1693-1699
2012
-
-
1
-
1
-
8
1
-
1
-
2
-
5
-
-
1
-
-
-
-
-
2
-
2
-
1
-
2
-
-
2
-
-
-
-
-
1
2
-
1
-
-
8
-
1
-
1
-
2
-
-
-
1
-
-
-
-
2
-
2
-
1
-
2
-
-
-
-
1
1
-
-
-
723913
Akita
Highly stable meso-diaminopime ...
Ureibacillus thermosphaericus, Ureibacillus thermosphaericus A1
AMB Express
1
43
2011
-
-
1
-
-
-
6
-
-
-
2
4
-
7
-
-
1
-
-
-
1
-
6
1
1
-
2
-
1
-
1
1
-
-
-
-
-
1
1
-
-
-
-
6
-
-
-
-
2
4
-
-
-
1
-
-
1
-
6
1
1
-
2
-
1
-
1
-
-
-
-
-
-
-
712938
Kind
Systems-wide metabolic pathway ...
Corynebacterium glutamicum
Metab. Eng.
12
341-351
2010
-
-
1
-
1
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
391524
Cirilli
The three-dimensional structur ...
Corynebacterium glutamicum, Lysinibacillus sphaericus
Protein Sci.
9
2034-2037
2000
-
-
-
1
-
-
1
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
2
1
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
391522
Wang
Substrate binding and conforma ...
Corynebacterium glutamicum
Protein Sci.
7
293-299
1998
-
-
1
-
-
-
-
2
-
-
1
-
-
4
-
-
1
1
-
-
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
2
-
-
1
-
-
-
-
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
391523
Scapin
Substrate and inhibitor bindin ...
Corynebacterium glutamicum
Biochemistry
37
3278-3285
1998
-
-
1
1
-
-
1
-
-
-
-
-
-
3
-
-
1
1
-
-
-
-
1
-
-
-
-
-
-
-
-
1
2
-
-
-
-
1
1
1
-
-
-
1
2
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
391520
Scapin
Three-dimensional structure of ...
Corynebacterium glutamicum
Biochemistry
35
13540-13551
1996
-
-
1
1
-
-
-
-
-
-
-
-
-
5
-
-
1
1
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
391521
Reddy
Expression, purification, and ...
Corynebacterium glutamicum
Proteins Struct. Funct. Genet.
25
514-516
1996
-
-
1
1
-
-
-
-
-
-
1
-
-
2
-
-
1
-
-
-
1
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
1
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
1
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2149
Abbott
-
Synthesis and testing of heter ...
Lysinibacillus sphaericus
J. Am. Chem. Soc.
116
6513-6520
1994
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
2
-
-
-
-
-
1
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2153
Chatterjee
Biosynthesis of lysine in plan ...
no activity in Chlamydomonas reinhardtii, no activity in Glycine max, no activity in Nicotiana tabacum, no activity in Zea mays
Plant Mol. Biol.
26
285-290
1994
-
-
-
-
-
-
-
-
-
-
-
-
-
9
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2145
Lam
Analogs of diaminopimelic acid ...
Lysinibacillus sphaericus
J. Biol. Chem.
263
11814-11819
1988
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4124
Yeh
General organization of the ge ...
Corynebacterium glutamicum, Corynebacterium glutamicum AS019
Mol. Gen. Genet.
212
105-111
1988
-
-
1
-
-
-
-
-
-
-
-
2
-
5
-
-
-
-
-
-
1
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
1
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390193
Cremer
Regulation of enzymes of lysin ...
Corynebacterium glutamicum
J. Gen. Microbiol.
134
3221-3229
1988
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
391502
Ishino
-
Cloning and sequencing of the ...
Corynebacterium glutamicum, Corynebacterium glutamicum RRL-5
Agric. Biol. Chem.
52
2903-2909
1988
-
-
1
-
-
-
-
-
-
-
1
2
-
2
-
-
-
-
-
-
2
-
4
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
1
2
-
-
-
-
-
-
2
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
391504
Ishino
Nucleotide sequence of the mes ...
Corynebacterium glutamicum
Nucleic Acids Res.
15
3917
1987
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390194
Bartlett
-
Regulation of the enzymes of l ...
Lysinibacillus sphaericus
J. Gen. Microbiol.
132
3169-3177
1986
-
-
-
-
-
-
3
-
-
-
-
1
-
1
-
-
-
-
-
-
3
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
3
-
-
-
-
-
1
-
-
-
-
-
-
3
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
391505
Misono
-
Characterization of meso-diami ...
Achromobacter polymorph, Achromobacter superficialis, Agrobacterium tumefaciens, Alcaligenes viscolactis, Bacillus amyloliquefaciens, Bacillus cereus, Bacillus licheniformis, Bacterium mycoides, Brevibacillus brevis, Brevibacterium sp., Corynebacterium ammoniagenes, Corynebacterium glutamicum, Corynebacterium pseudodiphtheriticum, Enterobacter cloacae, Flavobacterium suaveolens, Glutamicibacter protophormiae, Hafnia alvei, Kocuria rosea, Lysinibacillus sphaericus, Micrococcus luteus, Morganella morganii, no activity in Escherichia coli, no activity in Klebsiella pneumoniae, no activity in Staphylococcus aureus, Providencia rettgeri, Pseudomonas aeruginosa, Pseudomonas alkanolytica, Pseudomonas chlororaphis subsp. aureofaciens, Pseudomonas cruciviae, Pseudomonas fragi, Pseudomonas putida, Terrabacter tumescens
Agric. Biol. Chem.
50
2729-2734
1986
-
-
-
-
-
-
22
9
-
-
3
8
-
32
-
-
1
-
-
-
1
2
37
3
-
-
2
-
2
-
1
31
2
-
-
-
-
-
31
-
-
-
-
22
2
9
-
-
3
8
-
-
-
1
-
-
1
2
37
3
-
-
2
-
2
-
1
-
-
-
-
-
-
-
391507
Misono
-
Purification and properties of ...
Brevibacterium sp., Brevibacterium sp. ICR 7000
Agric. Biol. Chem.
50
1329-1330
1986
-
-
-
-
-
-
5
8
-
-
2
2
-
2
-
-
1
-
-
-
1
-
4
1
-
-
1
-
2
-
1
2
-
-
-
-
-
-
2
-
-
-
-
5
-
8
-
-
2
2
-
-
-
1
-
-
1
-
4
1
-
-
1
-
2
-
1
-
-
-
-
-
-
-
2139
Bartlett
-
Species of Bacillus that make ...
Lysinibacillus sphaericus, Paenibacillus macerans, Sporosarcina globispora, Sporosarcina pasteurii
J. Gen. Microbiol.
131
2145-2152
1985
-
-
-
-
-
-
-
-
-
-
-
4
-
4
-
-
-
-
-
-
3
-
8
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
3
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
391508
White
-
2,6-Diaminopimelate ...
Lysinibacillus sphaericus
Methods Enzym. Anal. , 3rd Ed. (Bergmeyer, H. U. , ed. )
8
377-383
1985
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
1
2
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
2
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
391510
Ishino
-
Involvement of meso-alpha,epsi ...
Corynebacterium glutamicum
Agric. Biol. Chem.
48
2257-2260
1984
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
391511
White
-
The essential role of diaminop ...
Lysinibacillus sphaericus
J. Gen. Microbiol.
129
739-749
1983
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
2
-
2
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
2
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
391512
Misono
-
meso-alpha,epsilon-Diaminopime ...
Lysinibacillus sphaericus
Agric. Biol. Chem.
45
1455-1460
1981
-
-
-
-
-
-
6
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
6
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
391513
Misono
Properties of meso-alpha,epsil ...
Lysinibacillus sphaericus
J. Biol. Chem.
255
10599-10605
1980
-
-
-
-
-
-
7
6
-
-
2
1
-
1
-
-
1
1
-
-
-
2
2
1
-
-
1
-
2
2
1
1
3
-
-
-
-
-
1
-
-
-
-
7
3
6
-
-
2
1
-
-
-
1
-
-
-
2
2
1
-
-
1
-
2
2
1
-
-
-
-
-
-
-
391514
Misono
-
Purification and properties of ...
Lysinibacillus sphaericus
Agric. Biol. Chem.
44
227-229
1980
-
-
-
-
-
-
-
3
-
-
1
1
-
1
-
-
1
-
-
-
1
-
2
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
3
-
-
1
1
-
-
-
1
-
-
1
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
391515
Misono
meso-alpha,epsilon-Diaminopime ...
Brevibacterium sp., Corynebacterium glutamicum, Erwinia aroidea, Lysinibacillus sphaericus, Proteus mirabilis, Proteus vulgaris, Pseudomonas fluorescens, Sarcina subflava
J. Bacteriol.
137
22-27
1979
-
-
-
-
-
-
-
-
-
-
-
4
-
8
-
-
-
-
-
-
-
-
12
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
12
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
391516
Misono
Occurrence of meso-alpha, epsi ...
Lysinibacillus sphaericus
Biochem. Biophys. Res. Commun.
72
89-93
1976
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
2
-
2
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
2
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-