BRENDA - Enzyme Database show
show all sequences of 1.4.1.16

Highly stable meso-diaminopimelate dehydrogenase from an Ureibacillus thermosphaericus strain A1 isolated from a Japanese compost: purification, characterization and sequencing

Akita, H.; Fujino, Y.; Doi, K.; Ohshima, T.; AMB Express 1, 43 (2011)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain Rosetta (DE3)
Ureibacillus thermosphaericus
Inhibitors
Inhibitors
Commentary
Organism
Structure
4-chloromercuribenzoate
complete inhibition at 0.1 mM
Ureibacillus thermosphaericus
Cu2+
strong inhibition at 1 mM
Ureibacillus thermosphaericus
HgCl2
complete inhibition at 0.1 mM
Ureibacillus thermosphaericus
L-cysteine
strong inhibition at 5 mM
Ureibacillus thermosphaericus
additional information
no inhibition by Zn2+, Co2+ and Ni2+ at 1 mM, and by 1 mM of D-lysine, L-lysine, EDTA, 2,2'-dipyridyl, NaN3, or iodoacetic acid
Ureibacillus thermosphaericus
Thioglycollate
strong inhibition at 5 mM
Ureibacillus thermosphaericus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40000
-
2 * 40000 SDS-PAGE
Ureibacillus thermosphaericus
80000
-
gel filtration
Ureibacillus thermosphaericus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
meso-2,6-diaminoheptanedioate + H2O + NADP+
Ureibacillus thermosphaericus
-
L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+
-
-
?
meso-2,6-diaminoheptanedioate + H2O + NADP+
Ureibacillus thermosphaericus A1
-
L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+
-
-
?
additional information
Ureibacillus thermosphaericus
meso-diaminopimelate dehydrogenase catalyzes the NAD(P)-dependent oxidative deamination of meso-diaminopimelate stereoselectively acting on the D-configuration of meso-diaminopimelate. The enzyme is highly selective for meso-diaminopimelate as the electron donor, and NADP+ but not NAD+ can serve as the electron acceptor
?
-
-
-
additional information
Ureibacillus thermosphaericus A1
meso-diaminopimelate dehydrogenase catalyzes the NAD(P)-dependent oxidative deamination of meso-diaminopimelate stereoselectively acting on the D-configuration of meso-diaminopimelate. The enzyme is highly selective for meso-diaminopimelate as the electron donor, and NADP+ but not NAD+ can serve as the electron acceptor
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Ureibacillus thermosphaericus
-
a thermophilic bacterium isolated from compost in Japan
-
Ureibacillus thermosphaericus A1
-
a thermophilic bacterium isolated from compost in Japan
-
Purification (Commentary)
Commentary
Organism
recombinant enzyme from Escherichia coli strain Rosetta (DE3) by heat treatment and affinity chromatography, native enzyme 47fold to homogeneity by ammonium sulfate fractionation, and hydrophobic interaction and anion exchange chromatography, followed by preparative slab PAGE
Ureibacillus thermosphaericus
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
8.28
-
purified enzyme, pH 7.2, 50°C
Ureibacillus thermosphaericus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
meso-2,6-diaminoheptanedioate + H2O + NADP+
-
723913
Ureibacillus thermosphaericus
L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+
-
-
-
?
meso-2,6-diaminoheptanedioate + H2O + NADP+
-
723913
Ureibacillus thermosphaericus A1
L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+
-
-
-
?
additional information
meso-diaminopimelate dehydrogenase catalyzes the NAD(P)-dependent oxidative deamination of meso-diaminopimelate stereoselectively acting on the D-configuration of meso-diaminopimelate. The enzyme is highly selective for meso-diaminopimelate as the electron donor, and NADP+ but not NAD+ can serve as the electron acceptor
723913
Ureibacillus thermosphaericus
?
-
-
-
-
additional information
no activity with DL-2-aminopimelate, D-glutamate, L-glutamate, D-aspartate, L-aspartate, D-alanine, L-alanine, D-valine, L-valine, D-lysine, L-lysine, D-phenylalanine, L-phenylalanine, D-leucine, L-leucine, D-threonine, L-threonine, D-serine, L-serine, D-tryptophan, L-tryptophan, D-cysteine, L-cysteine, D-histidine, L-histidine, D-methionine, D-arginine, D-proline, D-asparagine, D-glutamine, D-isoleucine, and D-ornithine, and with NAD+
723913
Ureibacillus thermosphaericus
?
-
-
-
-
additional information
meso-diaminopimelate dehydrogenase catalyzes the NAD(P)-dependent oxidative deamination of meso-diaminopimelate stereoselectively acting on the D-configuration of meso-diaminopimelate. The enzyme is highly selective for meso-diaminopimelate as the electron donor, and NADP+ but not NAD+ can serve as the electron acceptor
723913
Ureibacillus thermosphaericus A1
?
-
-
-
-
additional information
no activity with DL-2-aminopimelate, D-glutamate, L-glutamate, D-aspartate, L-aspartate, D-alanine, L-alanine, D-valine, L-valine, D-lysine, L-lysine, D-phenylalanine, L-phenylalanine, D-leucine, L-leucine, D-threonine, L-threonine, D-serine, L-serine, D-tryptophan, L-tryptophan, D-cysteine, L-cysteine, D-histidine, L-histidine, D-methionine, D-arginine, D-proline, D-asparagine, D-glutamine, D-isoleucine, and D-ornithine, and with NAD+
723913
Ureibacillus thermosphaericus A1
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
homodimer
2 * 40000 SDS-PAGE
Ureibacillus thermosphaericus
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
65
-
-
Ureibacillus thermosphaericus
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
60
-
purified enzyme, 30 min, pH 7.2, fully stable
Ureibacillus thermosphaericus
65
-
purified enzyme, 30 min, pH 7.2, loss of 50% activity
Ureibacillus thermosphaericus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
10.5
-
-
Ureibacillus thermosphaericus
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
5
11
purified enzyme, 30 min, 50°C, fully stable
Ureibacillus thermosphaericus
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
-
Ureibacillus thermosphaericus
Cloned(Commentary) (protein specific)
Commentary
Organism
DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain Rosetta (DE3)
Ureibacillus thermosphaericus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
-
Ureibacillus thermosphaericus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
4-chloromercuribenzoate
complete inhibition at 0.1 mM
Ureibacillus thermosphaericus
Cu2+
strong inhibition at 1 mM
Ureibacillus thermosphaericus
HgCl2
complete inhibition at 0.1 mM
Ureibacillus thermosphaericus
L-cysteine
strong inhibition at 5 mM
Ureibacillus thermosphaericus
additional information
no inhibition by Zn2+, Co2+ and Ni2+ at 1 mM, and by 1 mM of D-lysine, L-lysine, EDTA, 2,2'-dipyridyl, NaN3, or iodoacetic acid
Ureibacillus thermosphaericus
Thioglycollate
strong inhibition at 5 mM
Ureibacillus thermosphaericus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40000
-
2 * 40000 SDS-PAGE
Ureibacillus thermosphaericus
80000
-
gel filtration
Ureibacillus thermosphaericus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
meso-2,6-diaminoheptanedioate + H2O + NADP+
Ureibacillus thermosphaericus
-
L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+
-
-
?
meso-2,6-diaminoheptanedioate + H2O + NADP+
Ureibacillus thermosphaericus A1
-
L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+
-
-
?
additional information
Ureibacillus thermosphaericus
meso-diaminopimelate dehydrogenase catalyzes the NAD(P)-dependent oxidative deamination of meso-diaminopimelate stereoselectively acting on the D-configuration of meso-diaminopimelate. The enzyme is highly selective for meso-diaminopimelate as the electron donor, and NADP+ but not NAD+ can serve as the electron acceptor
?
-
-
-
additional information
Ureibacillus thermosphaericus A1
meso-diaminopimelate dehydrogenase catalyzes the NAD(P)-dependent oxidative deamination of meso-diaminopimelate stereoselectively acting on the D-configuration of meso-diaminopimelate. The enzyme is highly selective for meso-diaminopimelate as the electron donor, and NADP+ but not NAD+ can serve as the electron acceptor
?
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant enzyme from Escherichia coli strain Rosetta (DE3) by heat treatment and affinity chromatography, native enzyme 47fold to homogeneity by ammonium sulfate fractionation, and hydrophobic interaction and anion exchange chromatography, followed by preparative slab PAGE
Ureibacillus thermosphaericus
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
8.28
-
purified enzyme, pH 7.2, 50°C
Ureibacillus thermosphaericus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
meso-2,6-diaminoheptanedioate + H2O + NADP+
-
723913
Ureibacillus thermosphaericus
L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+
-
-
-
?
meso-2,6-diaminoheptanedioate + H2O + NADP+
-
723913
Ureibacillus thermosphaericus A1
L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+
-
-
-
?
additional information
meso-diaminopimelate dehydrogenase catalyzes the NAD(P)-dependent oxidative deamination of meso-diaminopimelate stereoselectively acting on the D-configuration of meso-diaminopimelate. The enzyme is highly selective for meso-diaminopimelate as the electron donor, and NADP+ but not NAD+ can serve as the electron acceptor
723913
Ureibacillus thermosphaericus
?
-
-
-
-
additional information
no activity with DL-2-aminopimelate, D-glutamate, L-glutamate, D-aspartate, L-aspartate, D-alanine, L-alanine, D-valine, L-valine, D-lysine, L-lysine, D-phenylalanine, L-phenylalanine, D-leucine, L-leucine, D-threonine, L-threonine, D-serine, L-serine, D-tryptophan, L-tryptophan, D-cysteine, L-cysteine, D-histidine, L-histidine, D-methionine, D-arginine, D-proline, D-asparagine, D-glutamine, D-isoleucine, and D-ornithine, and with NAD+
723913
Ureibacillus thermosphaericus
?
-
-
-
-
additional information
meso-diaminopimelate dehydrogenase catalyzes the NAD(P)-dependent oxidative deamination of meso-diaminopimelate stereoselectively acting on the D-configuration of meso-diaminopimelate. The enzyme is highly selective for meso-diaminopimelate as the electron donor, and NADP+ but not NAD+ can serve as the electron acceptor
723913
Ureibacillus thermosphaericus A1
?
-
-
-
-
additional information
no activity with DL-2-aminopimelate, D-glutamate, L-glutamate, D-aspartate, L-aspartate, D-alanine, L-alanine, D-valine, L-valine, D-lysine, L-lysine, D-phenylalanine, L-phenylalanine, D-leucine, L-leucine, D-threonine, L-threonine, D-serine, L-serine, D-tryptophan, L-tryptophan, D-cysteine, L-cysteine, D-histidine, L-histidine, D-methionine, D-arginine, D-proline, D-asparagine, D-glutamine, D-isoleucine, and D-ornithine, and with NAD+
723913
Ureibacillus thermosphaericus A1
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
homodimer
2 * 40000 SDS-PAGE
Ureibacillus thermosphaericus
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
65
-
-
Ureibacillus thermosphaericus
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
60
-
purified enzyme, 30 min, pH 7.2, fully stable
Ureibacillus thermosphaericus
65
-
purified enzyme, 30 min, pH 7.2, loss of 50% activity
Ureibacillus thermosphaericus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
10.5
-
-
Ureibacillus thermosphaericus
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
5
11
purified enzyme, 30 min, 50°C, fully stable
Ureibacillus thermosphaericus
Other publictions for EC 1.4.1.16
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
741705
Gao
A newly determined member of ...
Symbiobacterium thermophilum, Symbiobacterium thermophilum IAM14863
Appl. Environ. Microbiol.
83
e00476
2017
-
-
1
-
2
-
-
12
-
-
-
2
-
3
-
-
-
-
-
-
-
-
11
-
-
-
-
12
-
-
-
1
-
-
-
-
-
1
1
-
2
-
-
-
-
12
-
-
-
2
-
-
-
-
-
-
-
-
11
-
-
-
-
12
-
-
-
-
-
-
-
-
12
12
741514
Akita
Structural insight into the t ...
Ureibacillus thermosphaericus
Acta Crystallogr. Sect. D
71
1136-1146
2015
-
-
1
1
-
-
-
-
-
-
-
1
-
4
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
1
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
742282
Liu
Structural analysis reveals t ...
Clostridium tetani, Clostridium tetani E88
ChemBioChem
16
924-929
2015
-
-
1
1
1
-
-
-
-
-
-
2
-
3
-
-
1
-
-
-
3
-
8
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
1
1
-
-
-
-
-
-
-
-
2
-
-
-
1
-
-
3
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
742303
Zhao
Effect of residue Y76 on co-e ...
Symbiobacterium thermophilum
Chin. J. Biotechnol.
31
1108-1118
2015
-
-
-
-
1
-
-
-
-
-
-
1
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
743593
Stone
Identification of small-molec ...
Porphyromonas gingivalis, Porphyromonas gingivalis W83
PLoS ONE
10
e0141126
2015
-
-
1
-
-
-
11
2
-
-
-
2
-
5
-
-
1
-
-
-
-
-
2
1
-
-
-
-
-
-
-
1
-
-
4
-
-
1
1
-
-
-
4
11
-
2
-
-
-
2
-
-
-
1
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
742276
Liu
Structural and mutational stu ...
Symbiobacterium thermophilum
ChemBioChem
15
217-222
2014
-
-
1
1
9
-
-
-
-
-
-
1
-
2
-
-
1
-
-
-
-
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
1
9
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
724034
Gao
Engineering the meso-diaminopi ...
Symbiobacterium thermophilum
Appl. Environ. Microbiol.
79
5078-5081
2013
-
-
1
-
10
-
-
9
-
-
-
1
-
2
-
-
1
-
-
-
10
-
2
-
1
-
-
10
1
-
-
2
-
-
-
-
-
1
2
-
10
-
-
-
-
9
-
-
-
1
-
-
-
1
-
-
10
-
2
-
1
-
-
10
1
-
-
-
-
1
1
-
-
-
724026
Gao
A novel meso-Diaminopimelate d ...
Symbiobacterium thermophilum
Appl. Environ. Microbiol.
78
8595-8600
2012
-
1
1
-
3
-
-
8
-
-
-
2
-
3
-
-
1
-
-
-
-
-
4
-
1
-
4
8
2
-
-
2
-
-
-
-
1
1
2
-
3
-
-
-
-
8
-
-
-
2
-
-
-
1
-
-
-
-
4
-
1
-
4
8
2
-
-
-
-
1
1
-
-
-
724643
Akita
Creation of a thermostable NAD ...
Ureibacillus thermosphaericus, Ureibacillus thermosphaericus A1
Biotechnol. Lett.
34
1693-1699
2012
-
-
1
-
1
-
8
1
-
1
-
2
-
5
-
-
1
-
-
-
-
-
2
-
2
-
1
-
2
-
-
2
-
-
-
-
-
1
2
-
1
-
-
8
-
1
-
1
-
2
-
-
-
1
-
-
-
-
2
-
2
-
1
-
2
-
-
-
-
1
1
-
-
-
723913
Akita
Highly stable meso-diaminopime ...
Ureibacillus thermosphaericus, Ureibacillus thermosphaericus A1
AMB Express
1
43
2011
-
-
1
-
-
-
6
-
-
-
2
4
-
7
-
-
1
-
-
-
1
-
6
1
1
-
2
-
1
-
1
1
-
-
-
-
-
1
1
-
-
-
-
6
-
-
-
-
2
4
-
-
-
1
-
-
1
-
6
1
1
-
2
-
1
-
1
-
-
-
-
-
-
-
712938
Kind
Systems-wide metabolic pathway ...
Corynebacterium glutamicum
Metab. Eng.
12
341-351
2010
-
-
1
-
1
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
391524
Cirilli
The three-dimensional structur ...
Corynebacterium glutamicum, Lysinibacillus sphaericus
Protein Sci.
9
2034-2037
2000
-
-
-
1
-
-
1
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
2
1
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
391522
Wang
Substrate binding and conforma ...
Corynebacterium glutamicum
Protein Sci.
7
293-299
1998
-
-
1
-
-
-
-
2
-
-
1
-
-
4
-
-
1
1
-
-
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
2
-
-
1
-
-
-
-
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
391523
Scapin
Substrate and inhibitor bindin ...
Corynebacterium glutamicum
Biochemistry
37
3278-3285
1998
-
-
1
1
-
-
1
-
-
-
-
-
-
3
-
-
1
1
-
-
-
-
1
-
-
-
-
-
-
-
-
1
2
-
-
-
-
1
1
1
-
-
-
1
2
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
391520
Scapin
Three-dimensional structure of ...
Corynebacterium glutamicum
Biochemistry
35
13540-13551
1996
-
-
1
1
-
-
-
-
-
-
-
-
-
5
-
-
1
1
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
391521
Reddy
Expression, purification, and ...
Corynebacterium glutamicum
Proteins Struct. Funct. Genet.
25
514-516
1996
-
-
1
1
-
-
-
-
-
-
1
-
-
2
-
-
1
-
-
-
1
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
1
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
1
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2149
Abbott
-
Synthesis and testing of heter ...
Lysinibacillus sphaericus
J. Am. Chem. Soc.
116
6513-6520
1994
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
2
-
-
-
-
-
1
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2153
Chatterjee
Biosynthesis of lysine in plan ...
no activity in Chlamydomonas reinhardtii, no activity in Glycine max, no activity in Nicotiana tabacum, no activity in Zea mays
Plant Mol. Biol.
26
285-290
1994
-
-
-
-
-
-
-
-
-
-
-
-
-
9
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2145
Lam
Analogs of diaminopimelic acid ...
Lysinibacillus sphaericus
J. Biol. Chem.
263
11814-11819
1988
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4124
Yeh
General organization of the ge ...
Corynebacterium glutamicum, Corynebacterium glutamicum AS019
Mol. Gen. Genet.
212
105-111
1988
-
-
1
-
-
-
-
-
-
-
-
2
-
5
-
-
-
-
-
-
1
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
1
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390193
Cremer
Regulation of enzymes of lysin ...
Corynebacterium glutamicum
J. Gen. Microbiol.
134
3221-3229
1988
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
391502
Ishino
-
Cloning and sequencing of the ...
Corynebacterium glutamicum, Corynebacterium glutamicum RRL-5
Agric. Biol. Chem.
52
2903-2909
1988
-
-
1
-
-
-
-
-
-
-
1
2
-
2
-
-
-
-
-
-
2
-
4
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
1
2
-
-
-
-
-
-
2
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
391504
Ishino
Nucleotide sequence of the mes ...
Corynebacterium glutamicum
Nucleic Acids Res.
15
3917
1987
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390194
Bartlett
-
Regulation of the enzymes of l ...
Lysinibacillus sphaericus
J. Gen. Microbiol.
132
3169-3177
1986
-
-
-
-
-
-
3
-
-
-
-
1
-
1
-
-
-
-
-
-
3
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
3
-
-
-
-
-
1
-
-
-
-
-
-
3
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
391505
Misono
-
Characterization of meso-diami ...
Achromobacter polymorph, Achromobacter superficialis, Agrobacterium tumefaciens, Alcaligenes viscolactis, Bacillus amyloliquefaciens, Bacillus cereus, Bacillus licheniformis, Bacterium mycoides, Brevibacillus brevis, Brevibacterium sp., Corynebacterium ammoniagenes, Corynebacterium glutamicum, Corynebacterium pseudodiphtheriticum, Enterobacter cloacae, Flavobacterium suaveolens, Glutamicibacter protophormiae, Hafnia alvei, Kocuria rosea, Lysinibacillus sphaericus, Micrococcus luteus, Morganella morganii, no activity in Escherichia coli, no activity in Klebsiella pneumoniae, no activity in Staphylococcus aureus, Providencia rettgeri, Pseudomonas aeruginosa, Pseudomonas alkanolytica, Pseudomonas chlororaphis subsp. aureofaciens, Pseudomonas cruciviae, Pseudomonas fragi, Pseudomonas putida, Terrabacter tumescens
Agric. Biol. Chem.
50
2729-2734
1986
-
-
-
-
-
-
22
9
-
-
3
8
-
32
-
-
1
-
-
-
1
2
37
3
-
-
2
-
2
-
1
31
2
-
-
-
-
-
31
-
-
-
-
22
2
9
-
-
3
8
-
-
-
1
-
-
1
2
37
3
-
-
2
-
2
-
1
-
-
-
-
-
-
-
391507
Misono
-
Purification and properties of ...
Brevibacterium sp., Brevibacterium sp. ICR 7000
Agric. Biol. Chem.
50
1329-1330
1986
-
-
-
-
-
-
5
8
-
-
2
2
-
2
-
-
1
-
-
-
1
-
4
1
-
-
1
-
2
-
1
2
-
-
-
-
-
-
2
-
-
-
-
5
-
8
-
-
2
2
-
-
-
1
-
-
1
-
4
1
-
-
1
-
2
-
1
-
-
-
-
-
-
-
2139
Bartlett
-
Species of Bacillus that make ...
Lysinibacillus sphaericus, Paenibacillus macerans, Sporosarcina globispora, Sporosarcina pasteurii
J. Gen. Microbiol.
131
2145-2152
1985
-
-
-
-
-
-
-
-
-
-
-
4
-
4
-
-
-
-
-
-
3
-
8
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
3
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
391508
White
-
2,6-Diaminopimelate ...
Lysinibacillus sphaericus
Methods Enzym. Anal. , 3rd Ed. (Bergmeyer, H. U. , ed. )
8
377-383
1985
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
1
2
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
2
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
391510
Ishino
-
Involvement of meso-alpha,epsi ...
Corynebacterium glutamicum
Agric. Biol. Chem.
48
2257-2260
1984
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
391511
White
-
The essential role of diaminop ...
Lysinibacillus sphaericus
J. Gen. Microbiol.
129
739-749
1983
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
2
-
2
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
2
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
391512
Misono
-
meso-alpha,epsilon-Diaminopime ...
Lysinibacillus sphaericus
Agric. Biol. Chem.
45
1455-1460
1981
-
-
-
-
-
-
6
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
6
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
391513
Misono
Properties of meso-alpha,epsil ...
Lysinibacillus sphaericus
J. Biol. Chem.
255
10599-10605
1980
-
-
-
-
-
-
7
6
-
-
2
1
-
1
-
-
1
1
-
-
-
2
2
1
-
-
1
-
2
2
1
1
3
-
-
-
-
-
1
-
-
-
-
7
3
6
-
-
2
1
-
-
-
1
-
-
-
2
2
1
-
-
1
-
2
2
1
-
-
-
-
-
-
-
391514
Misono
-
Purification and properties of ...
Lysinibacillus sphaericus
Agric. Biol. Chem.
44
227-229
1980
-
-
-
-
-
-
-
3
-
-
1
1
-
1
-
-
1
-
-
-
1
-
2
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
3
-
-
1
1
-
-
-
1
-
-
1
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
391515
Misono
meso-alpha,epsilon-Diaminopime ...
Brevibacterium sp., Corynebacterium glutamicum, Erwinia aroidea, Lysinibacillus sphaericus, Proteus mirabilis, Proteus vulgaris, Pseudomonas fluorescens, Sarcina subflava
J. Bacteriol.
137
22-27
1979
-
-
-
-
-
-
-
-
-
-
-
4
-
8
-
-
-
-
-
-
-
-
12
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
12
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
391516
Misono
Occurrence of meso-alpha, epsi ...
Lysinibacillus sphaericus
Biochem. Biophys. Res. Commun.
72
89-93
1976
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
2
-
2
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
2
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-