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Literature summary for 1.4.1.13 extracted from

  • Mei, B.; Jiao, R.
    Purification and properties of glutamate synthase from Nocardia mediterranei (1988), J. Bacteriol., 170, 1940-1944.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.4.1.13

General Stability

General Stability Organism
2 mM alpha-ketoglutarate, 10 mM beta-mercaptoethanol and 10% glycerol when present together markedly enhance stability Amycolatopsis mediterranei
unprotected enzyme is unstable, losing 65% of its activity in 24 hours at 4°C Amycolatopsis mediterranei

Inhibitors

Inhibitors Comment Organism Structure
D-glutamate
-
 Amycolatopsis mediterranei
glycine less than 50% inhibition at 5 mM Amycolatopsis mediterranei
isocitrate 35% inhibition at 5 mM Amycolatopsis mediterranei
L-alanine less than 50% inhibition at 5 mM Amycolatopsis mediterranei
L-asparagine less than 50% inhibition at 5 mM Amycolatopsis mediterranei
L-glutamate potent inhibitor Amycolatopsis mediterranei
L-histidine less than 50% inhibition at 5 mM Amycolatopsis mediterranei
L-leucine less than 50% inhibition at 5 mM Amycolatopsis mediterranei
L-malate more than 50% inhibition at 5 mM Amycolatopsis mediterranei
L-methionine shows no effect Amycolatopsis mediterranei
L-serine less than 50% inhibition at 5 mM Amycolatopsis mediterranei
L-tryptophan strong inhibitor Amycolatopsis mediterranei
NADP+ potent inhibitor Amycolatopsis mediterranei
succinate 25% inhibition at 5 mM Amycolatopsis mediterranei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.053
-
 alpha-ketoglutarate
-
 Amycolatopsis mediterranei
0.077
-
 L-glutamine
-
 Amycolatopsis mediterranei
0.11
-
 NADPH
-
 Amycolatopsis mediterranei

Metals/Ions

Metals/Ions Comment Organism Structure
Iron iron-sulfur protein Amycolatopsis mediterranei
Iron contains 7.5 mol of nonheme iron Amycolatopsis mediterranei

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
 1 * 145000 + 1 * 55000, SDS-PAGE Amycolatopsis mediterranei
145000
-
 1 * 145000 + 1 * 55000, SDS-PAGE Amycolatopsis mediterranei
195000
-
 sucrose density gradient centrifugation Amycolatopsis mediterranei

Organism

Organism UniProt Comment Textmining
Amycolatopsis mediterranei
-
-
-

Oxidation Stability

Oxidation Stability Organism
presence of reducing agents is essential for stabilization Amycolatopsis mediterranei

Purification (Commentary)

Purification (Comment) Organism
using protamine sulfate precipitation, ammonium sulfate precipitation, column chromatography on DE52, DEAE-Sephadex, hydroxyapatite, phenyl-Sepharose CL-4B and Bio-Gel filtration Amycolatopsis mediterranei

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.48
-
-
 Amycolatopsis mediterranei

Storage Stability

Storage Stability Organism
4°C, unprotected enzyme loses 65% of activity after 24 h Amycolatopsis mediterranei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamine + 2-oxoglutarate + NADPH + H+ in the reverse reaction ammonia can act instead of glutamine, but more slowly Amycolatopsis mediterranei L-glutamate + NADP+
-
 ?
NH3 + 2-oxoglutarate + NADPH + H+ 14% relative activity to L-glutamine Amycolatopsis mediterranei L-glutamate + NADP+
-
 ?

Subunits

Subunits Comment Organism
dimer 1 * 145000 + 1 * 55000, SDS-PAGE Amycolatopsis mediterranei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8.5 broad optimum Amycolatopsis mediterranei

Cofactor

Cofactor Comment Organism Structure
flavin contains FAD and FMN Amycolatopsis mediterranei
flavin ratio FAD: FMN is 1.1 Amycolatopsis mediterranei
additional information the enzyme contains 7.2 mol of acid-labile sulfur per 200,000 g of protein Amycolatopsis mediterranei
NADH no activity with NADH Amycolatopsis mediterranei
NADPH
-
 Amycolatopsis mediterranei