Cloned (Comment) | Organism |
---|---|
- |
Mycobacterium tuberculosis |
Protein Variants | Comment | Organism |
---|---|---|
F94S | mutation alters its substrate specificity pattern, enabling activity toward a range of larger amino acids | Mycobacterium tuberculosis |
F94S/Y117L | mutant shows improved activity toward hydrophobic amino acids | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
24 | - |
L-serine | wild-type, pH 10.2, temperature not specified in the publication | Mycobacterium tuberculosis | |
53 | - |
L-alanine | wild-type, pH 10.2, temperature not specified in the publication | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WQB1 | - |
- |
Mycobacterium tuberculosis H37Rv | P9WQB1 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-2-amino-4-pentenoate + H2O + NAD+ | - |
Mycobacterium tuberculosis | 2-oxo-4-pentenoate + NH3 + NADH | - |
? | |
L-2-amino-4-pentenoate + H2O + NAD+ | - |
Mycobacterium tuberculosis H37Rv | 2-oxo-4-pentenoate + NH3 + NADH | - |
? | |
L-alanine + H2O + NAD+ | - |
Mycobacterium tuberculosis | pyruvate + NH3 + NADH + H+ | - |
? | |
L-alanine + H2O + NAD+ | - |
Mycobacterium tuberculosis H37Rv | pyruvate + NH3 + NADH + H+ | - |
? | |
L-homophenylalanine + H2O + NADH + H+ | - |
Mycobacterium tuberculosis | 2-oxo-4-phenylbutanoate + NH3 + NAD+ | - |
? | |
L-homoserine + H2O + NAD+ | - |
Mycobacterium tuberculosis | 4-hydroxy-2-oxobutanoate + NH3 + NADH | - |
? | |
L-leucine + H2O + NAD+ | - |
Mycobacterium tuberculosis | 4-methyl-2-oxopentanoate + NH3 + NADH | - |
? | |
L-methionine + H2O + NAD+ | - |
Mycobacterium tuberculosis | 4-(methylsulfanyl)-2-oxobutanoate + NH3 + NADH | - |
? | |
L-norleucine + H2O + NADH + H+ | - |
Mycobacterium tuberculosis | 2-oxohexanoate + NH3 + NAD+ | - |
? | |
L-norvaline + H2O + NAD+ | - |
Mycobacterium tuberculosis | 2-oxopentanoate + NH3 + NADH | - |
? | |
L-norvaline + H2O + NAD+ | - |
Mycobacterium tuberculosis H37Rv | 2-oxopentanoate + NH3 + NADH | - |
? | |
L-serine + H2O + NAD+ | - |
Mycobacterium tuberculosis | 3-hydroxypyruvate + NH3 + NADH | - |
? | |
L-serine + H2O + NAD+ | - |
Mycobacterium tuberculosis H37Rv | 3-hydroxypyruvate + NH3 + NADH | - |
? | |
L-valine + H2O + NAD+ | - |
Mycobacterium tuberculosis | 3-methyl-2-oxobutanoate + NH3 + NADH | - |
? | |
L-valine + H2O + NAD+ | - |
Mycobacterium tuberculosis H37Rv | 3-methyl-2-oxobutanoate + NH3 + NADH | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.24 | - |
L-serine | wild-type, pH 10.2, temperature not specified in the publication | Mycobacterium tuberculosis | |
7.5 | - |
L-alanine | wild-type, pH 10.2, temperature not specified in the publication | Mycobacterium tuberculosis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00035 | - |
L-alanine | mutant F94S/Y117L, pH 10.2, temperature not specified in the publication | Mycobacterium tuberculosis | |
0.0004 | - |
L-norvaline | wild-type, pH 10.2, temperature not specified in the publication | Mycobacterium tuberculosis | |
0.00088 | - |
L-leucine | mutant F94S, pH 10.2, temperature not specified in the publication | Mycobacterium tuberculosis | |
0.0015 | - |
L-2-amino-4-pentenoate | wild-type, pH 10.2, temperature not specified in the publication | Mycobacterium tuberculosis | |
0.0016 | - |
L-methionine | mutant F94S, pH 10.2, temperature not specified in the publication | Mycobacterium tuberculosis | |
0.0017 | - |
L-norvaline | mutant F94S, pH 10.2, temperature not specified in the publication | Mycobacterium tuberculosis | |
0.0022 | - |
L-alanine | mutant F94S, pH 10.2, temperature not specified in the publication | Mycobacterium tuberculosis | |
0.0026 | - |
L-methionine | mutant F94S/Y117L, pH 10.2, temperature not specified in the publication | Mycobacterium tuberculosis | |
0.0027 | - |
L-leucine | mutant F94S/Y117L, pH 10.2, temperature not specified in the publication | Mycobacterium tuberculosis | |
0.004 | - |
L-2-amino-4-pentenoate | mutant F94S, pH 10.2, temperature not specified in the publication | Mycobacterium tuberculosis | |
0.0046 | - |
L-valine | wild-type, pH 10.2, temperature not specified in the publication | Mycobacterium tuberculosis | |
0.005 | - |
L-2-amino-4-pentenoate | mutant F94S/Y117L, pH 10.2, temperature not specified in the publication | Mycobacterium tuberculosis | |
0.006 | - |
L-norleucine | mutant F94S, pH 10.2, temperature not specified in the publication | Mycobacterium tuberculosis | |
0.01 | - |
L-serine | wild-type, pH 10.2, temperature not specified in the publication | Mycobacterium tuberculosis | |
0.013 | - |
L-norleucine | mutant F94S/Y117L, pH 10.2, temperature not specified in the publication | Mycobacterium tuberculosis | |
0.015 | - |
L-homoserine | wild-type, pH 10.2, temperature not specified in the publication | Mycobacterium tuberculosis | |
0.019 | - |
L-homophenylalanine | mutant F94S, pH 10.2, temperature not specified in the publication | Mycobacterium tuberculosis | |
0.037 | - |
L-homophenylalanine | mutant F94S/Y117L, pH 10.2, temperature not specified in the publication | Mycobacterium tuberculosis | |
0.04 | - |
L-norvaline | mutant F94S/Y117L, pH 10.2, temperature not specified in the publication | Mycobacterium tuberculosis | |
1.4 | - |
L-alanine | wild-type, pH 10.2, temperature not specified in the publication | Mycobacterium tuberculosis |