Cloned (Comment) | Organism |
---|---|
gene ald, expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Priestia megaterium |
Crystallization (Comment) | Organism |
---|---|
purified recombinant His6-tagged enzyme, hanging drop vapour diffusion method, 15 mg/ml protein in 20 mM Tris-HCl, pH 8.0, mixed with 0.1 M HEPES, pH 8.0, 12% w/v PEG 8000, and 8% v/v ethylene glycol, 4°C, method optimization, X-ray diffraction structure determination and analysis at 2.35 A resolution | Priestia megaterium |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
x * 42000, recombinant His6-tagged enzme, SDS-PAGE | Priestia megaterium |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-alanine + H2O + NAD+ | Priestia megaterium | - |
pyruvate + NH3 + NADH + H+ | - |
r | |
L-alanine + H2O + NAD+ | Priestia megaterium WSH-002 | - |
pyruvate + NH3 + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Priestia megaterium | A0A0L1M9S8 | gene ald | - |
Priestia megaterium WSH-002 | A0A0L1M9S8 | gene ald | - |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration | Priestia megaterium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-alanine + H2O + NAD+ | - |
Priestia megaterium | pyruvate + NH3 + NADH + H+ | - |
r | |
L-alanine + H2O + NAD+ | - |
Priestia megaterium WSH-002 | pyruvate + NH3 + NADH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 42000, recombinant His6-tagged enzme, SDS-PAGE | Priestia megaterium |
Synonyms | Comment | Organism |
---|---|---|
L-AlaDH | - |
Priestia megaterium |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Priestia megaterium | |
NADH | - |
Priestia megaterium |