Cloned (Comment) | Organism |
---|---|
- |
Mycobacterium tuberculosis |
Crystallization (Comment) | Organism |
---|---|
using the hanging-drop method crystal structures of the apo enzyme as also a ternary complex with NAD and pyruvate is reported. Each chain of the enzyme can be divided into catalytic and NAD-binding domains, respectively. The individual subunits associate into a hexamer with the catalytic domains on the outside as observed in the apo and holo enzyme crystals. The structures have captured open and closed conformations of the enzyme and clarify that a domain rearrangement step must take place during the reaction as opposed to alterations in intersubunit interactions | Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
SDS-PAGE | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WQB1 | - |
- |
Mycobacterium tuberculosis H37Rv | P9WQB1 | - |
- |
Purification (Comment) | Organism |
---|---|
using affinity chromatography | Mycobacterium tuberculosis |
Storage Stability | Organism |
---|---|
4°C, stable up to 1 week without degradation | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate + NH3 + NADH | the structural analysis leads to the identification of a water molecule which is hydrogen bonded to the active site His 96 and probably drives the conversion of the iminopyruvate intermediate to a carbinolamine | Mycobacterium tuberculosis | L-alanine + H2O + NAD+ | - |
? | |
pyruvate + NH3 + NADH | the structural analysis leads to the identification of a water molecule which is hydrogen bonded to the active site His 96 and probably drives the conversion of the iminopyruvate intermediate to a carbinolamine | Mycobacterium tuberculosis H37Rv | L-alanine + H2O + NAD+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 40000 Da, gel filtration and crystal structure | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
alanine dehydrogenase | - |
Mycobacterium tuberculosis |
ALD | - |
Mycobacterium tuberculosis |
MtbALD | - |
Mycobacterium tuberculosis |
Rv2780 | - |
Mycobacterium tuberculosis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Mycobacterium tuberculosis |