Literature summary for 1.4.1.1 extracted from

Agren, D.; Stehr, M.; Berthold, C.L.; Kapoor, S.; Oehlmann, W.; Singh, M.; Schneider, G.
Three-dimensional structures of apo- and holo-L-alanine dehydrogenase from Mycobacterium tuberculosis reveal conformational changes upon coenzyme binding (2008), J. Mol. Biol., 377, 1161-1173.

Search for more literature for 1.4.1.1

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Mycobacterium tuberculosis

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop vapour diffusion method, crystals of apo-AlaDH are grown using 0.4 M ammonium hydrogenphosphate as reservoir solution at 20°C	Mycobacterium tuberculosis

Protein Variants

Protein Variants	Comment	Organism
D270A	inactive	Mycobacterium tuberculosis
D270N	inactive, the bifurcated hydrogen bond between Asp270 and the ribose of NAD is replaced by a single hydrogen bond	Mycobacterium tuberculosis
H96A	inactive	Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.76	-	pyruvate	wild type enzyme, in 1 M NH4Cl/NH3, pH 7.4, at 25°C	Mycobacterium tuberculosis
15.64	-	L-Ala	wild type enzyme, in 125 mM glycine/KOH (pH 10.2), at 25°C	Mycobacterium tuberculosis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
38988	-	6 * 38988, predicted molecular mass	Mycobacterium tuberculosis

Organism

Organism	UniProt	Comment	Textmining
Mycobacterium tuberculosis	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA resin column chromatography	Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-Ala + H2O + NAD+	-	Mycobacterium tuberculosis	pyruvate + NH3 + NADH	-	r

Subunits

Subunits	Comment	Organism
homohexamer	in solution, gel filtration	Mycobacterium tuberculosis
homohexamer	6 * 38988, predicted molecular mass	Mycobacterium tuberculosis

Synonyms

Synonyms	Comment	Organism
L-AlaDH	-	Mycobacterium tuberculosis

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
126	-	L-Ala	wild type enzyme, in 125 mM glycine/KOH (pH 10.2), at 25°C	Mycobacterium tuberculosis
694	-	pyruvate	wild type enzyme, in 1 M NH4Cl/NH3, pH 7.4, at 25°C	Mycobacterium tuberculosis

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	-	Mycobacterium tuberculosis

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Release 2023.1 (January 2023)