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Literature summary for 1.4.1.1 extracted from

  • Ashida, H.; Galkin, A.; Kulakova, L.; Sawa, Y.; Nakajima, N.; Esaki, N.
    Conversion of cofactor specificities of alanine dehydrogenases by site-directed mutagenesis (2004), J. Mol. Catal. B, 30, 173-176.
No PubMed abstract available
Search for more literature for 1.4.1.1

Protein Variants

Protein Variants Comment Organism
D198A mutant enzyme shows higher efficiency with NADP+ as coenzyme than with NAD+ Shewanella sp.
D198G mutant enzyme shows higher efficiency with NADP+ as coenzyme than with NAD+ Shewanella sp.
D198L mutant enzyme shows higher efficiency with NADP+ as coenzyme than with NAD+ Shewanella sp.
D198R acts also on NADP+ Phormidium lapideum
D198V mutant enzyme shows higher efficiency with NADP+ as coenzyme than with NAD+ Shewanella sp.
R199I specific for NAD+ as the wild-type enzyme Phormidium lapideum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
 NAD+ 30°C, mutant enzyme R199I Shewanella sp.
0.035
-
 NAD+ 30°C, wild-type enzyme Shewanella sp.
0.036
-
 NAD+ 45°C, wild-type enzyme Phormidium lapideum
0.15
-
 NAD+ 45°C, mutant enzyme I198R Phormidium lapideum
0.35
-
 NADP+ 30°C, mutant enzyme D198AI Shewanella sp.
0.67
-
 NADP+ 30°C, mutant enzyme D198G Shewanella sp.
1.1
-
 NADP+ 30°C, mutant enzyme D198V Shewanella sp.
2.1
-
 NADP+ 30°C, mutant enzyme D198L Shewanella sp.
2.8
-
 NADP+ 45°C, mutant enzyme I198R Phormidium lapideum
3
-
 NAD+ 30°C, mutant enzyme D198G Shewanella sp.
4
-
 NADP+ 30°C, wild-type enzyme Shewanella sp.
5.2
-
 NAD+ 30°C, mutant enzyme D198V Shewanella sp.
5.6
-
 NAD+ 30°C, mutant enzyme D198AI Shewanella sp.
11.4
-
 NAD+ 30°C, mutant enzyme D198L Shewanella sp.

Organism

Organism UniProt Comment Textmining
Phormidium lapideum O52942 Ac10
-
Phormidium lapideum Ac10 O52942 Ac10
-
Shewanella sp.
-
 Ac10
-
Shewanella sp. Ac10
-
 Ac10
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Ala + H2O + NAD+
-
 Shewanella sp. pyruvate + NH3 + NADH
-
 ?
L-Ala + H2O + NAD+
-
 Phormidium lapideum pyruvate + NH3 + NADH
-
 ?
L-Ala + H2O + NAD+
-
 Phormidium lapideum Ac10 pyruvate + NH3 + NADH
-
 ?
L-Ala + H2O + NAD+
-
 Shewanella sp. Ac10 pyruvate + NH3 + NADH
-
 ?
L-Ala + H2O + NADP+ wild-type enzyme and mutant enzyme R199I acts specifically on NAD+, mutant enzyme I198R is also active with NADP+ Phormidium lapideum pyruvate + NH3 + NADPH
-
 ?
L-Ala + H2O + NADP+ wild-type enzyme shows high activity with NAD+ and low activity with NADP+. The mutant enzymes D198G, D198A, D198V and D198L show higher efficiency with NADP+ than with NAD+ as coenzyme Shewanella sp. pyruvate + NH3 + NADPH
-
 ?
L-Ala + H2O + NADP+ wild-type enzyme and mutant enzyme R199I acts specifically on NAD+, mutant enzyme I198R is also active with NADP+ Phormidium lapideum Ac10 pyruvate + NH3 + NADPH
-
 ?
L-Ala + H2O + NADP+ wild-type enzyme shows high activity with NAD+ and low activity with NADP+. The mutant enzymes D198G, D198A, D198V and D198L show higher efficiency with NADP+ than with NAD+ as coenzyme Shewanella sp. Ac10 pyruvate + NH3 + NADPH
-
 ?

Synonyms

Synonyms Comment Organism
PlaAlaDH
-
 Phormidium lapideum
SheAlaDH
-
 Shewanella sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3
-
 NADP+ 45°C, mutant enzyme I198R Phormidium lapideum
1.6
-
 NADP+ 30°C, wild-type enzyme Shewanella sp.
1.9
-
 NAD+ 30°C, mutant enzyme D198L Shewanella sp.
4.8
-
 NADP+ 30°C, mutant enzyme D198L Shewanella sp.
9.5
-
 NAD+ 30°C, mutant enzyme R199I Shewanella sp.
24.1
-
 NADP+ 30°C, mutant enzyme D198G Shewanella sp.
34.7
-
 NAD+ 30°C, wild-type enzyme Shewanella sp.
37.8
-
 NADP+ 30°C, mutant enzyme D198AI Shewanella sp.
41.2
-
 NAD+ 30°C, mutant enzyme D198G Shewanella sp.
41.2
-
 NAD+ 30°C, mutant enzyme D198V Shewanella sp.
43.5
-
 NAD+ 30°C, mutant enzyme D198AI Shewanella sp.
50.2
-
 NADP+ 30°C, mutant enzyme D198V Shewanella sp.
95.4
-
 NAD+ 45°C, mutant enzyme I198R Phormidium lapideum
95.5
-
 NAD+ 45°C, wild-type enzyme Phormidium lapideum

Cofactor

Cofactor Comment Organism Structure
NAD+ wild-type enzyme and mutant enzyme R199I acts specifically on NAD+, mutant enzyme I198R is also active with NADP+ Phormidium lapideum
NAD+ wild-type enzyme shows high activity with NAD+ and low activity with NADP+. The mutant enzymes D198G, D198A, D198V and D198L show higher efficiency with NADP+ than with NAD+ as coenzyme Shewanella sp.
NADP+ wild-type enzyme and mutant enzyme R199I acts specifically on NAD+, mutant enzyme I198R is also active with NADP+ Phormidium lapideum
NADP+ wild-type enzyme shows high activity with NAD+ and low activity with NADP+. The mutant enzymes D198G, D198A, D198V and D198L show higher efficiency with NADP+ than with NAD+ as coenzyme Shewanella sp.