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Literature summary for 1.4.1.1 extracted from

  • Porumb, H.; Vancea, D.; Muresan, L.; Presecan, E.; Lascu, I.; Petrescu, I.; Porumb, T.; Pop, R.; Barzu, O.
    Structural and catalytic properties of L-alanine dehydrogenase from Bacillus cereus (1987), J. Biol. Chem., 262, 4610-4615.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Cibacron blue
-
Bacillus cereus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
6 * 42000, SDS-PAGE Bacillus cereus
255000
-
non-denaturing PAGE Bacillus cereus

Organism

Organism UniProt Comment Textmining
Bacillus cereus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Bacillus cereus

Renatured (Commentary)

Renatured (Comment) Organism
the ability of denatured immobilized subunits to pick up subunits from solution proves their capacity to fold back to the native conformation after urea treatment Bacillus cereus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Ala + H2O + NAD+
-
Bacillus cereus pyruvate + NH3 + NADH
-
r

Subunits

Subunits Comment Organism
hexamer 6 * 42000, SDS-PAGE Bacillus cereus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5 9 reductive amination Bacillus cereus
10.5 11 oxidative deamination Bacillus cereus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Bacillus cereus
NADH
-
Bacillus cereus