BRENDA - Enzyme Database show
show all sequences of 1.3.99.B2

Biochemical characterization of AtuD from Pseudomonas aeruginosa, the first member of a new subgroup of acyl-CoA dehydrogenases with specificity for citronellyl-CoA

Foerster-Fromme, K.; Chattopadhyay, A.; Jendrossek, D.; Microbiology 154, 789-796 (2008)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli; expression in Escherichia coli
Pseudomonas aeruginosa
Engineering
Amino acid exchange
Commentary
Organism
additional information
gene insertion mutant is not able to grow on citronellol, geraniol, citronellate, geranylate as sole carbon source; gene insertion mutant mutants grow slightly more slowly on citronellol and citronellate but show normal growth on geraniol and geranylate
Pseudomonas aeruginosa
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0016
-
citronellyl-CoA
pH 7.0, 30C
Pseudomonas aeruginosa
0.018
-
citronellyl-CoA
pH 7.0, 30C
Pseudomonas aeruginosa
0.13
-
octanoyl-CoA
pH 7.0, 30C
Pseudomonas aeruginosa
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
41000
-
x * 41000, SDS-PAGE, x * 42000, calculated; x * 41000, SDS-PAGE, x * 42700, calculated
Pseudomonas aeruginosa
42000
-
x * 41000, SDS-PAGE, x * 42000, calculated
Pseudomonas aeruginosa
42700
-
x * 41000, SDS-PAGE, x * 42700, calculated
Pseudomonas aeruginosa
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas aeruginosa
Q9HZV8
gene atud; specific expression in cells growing on acyclic terpenes, absent in isovalerate- or succinate-grown cells
-
Pseudomonas aeruginosa
Q9I3H8
gene PA1535, second citronellyl-CoA dehydrogenase, less specific; specific expression in cells growing on acyclic terpenes, absent in isovalerate- or succinate-grown cells
-
Purification (Commentary)
Commentary
Organism
recombinant protein; recombinant protein
Pseudomonas aeruginosa
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
citronellyl-CoA + 2,6-dichlorophenolindophenol
-
694044
Pseudomonas aeruginosa
cis-geranyl-CoA + reduced 2,6-dichlorophenolindophenol
-
-
-
?
additional information
no substrates: 5-methylhex-4-enoyl-CoA or isovaleryl-CoA
694044
Pseudomonas aeruginosa
?
-
-
-
-
additional information
no substrates: octanoyl-CoA, 5-methylhex-4-enoyl-CoA or isovaleryl-CoA
694044
Pseudomonas aeruginosa
?
-
-
-
-
octanoyl-CoA + 2,6-dichlorophenolindophenol
-
694044
Pseudomonas aeruginosa
cis-geranyl-CoA + reduced 2,6-dichlorophenolindophenol
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 41000, SDS-PAGE, x * 42000, calculated; x * 41000, SDS-PAGE, x * 42700, calculated
Pseudomonas aeruginosa
Cofactor
Cofactor
Commentary
Organism
Structure
FAD
purified and concentrated protein is yellow, and spectral analysis confirms the presence of FAD as cofactor; purified and concentrated protein is yellow, and spectral analysis confirms the presence of FAD as cofactor
Pseudomonas aeruginosa
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Pseudomonas aeruginosa
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FAD
purified and concentrated protein is yellow, and spectral analysis confirms the presence of FAD as cofactor
Pseudomonas aeruginosa
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
gene insertion mutant is not able to grow on citronellol, geraniol, citronellate, geranylate as sole carbon source
Pseudomonas aeruginosa
additional information
gene insertion mutant mutants grow slightly more slowly on citronellol and citronellate but show normal growth on geraniol and geranylate
Pseudomonas aeruginosa
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0016
-
citronellyl-CoA
pH 7.0, 30C
Pseudomonas aeruginosa
0.018
-
citronellyl-CoA
pH 7.0, 30C
Pseudomonas aeruginosa
0.13
-
octanoyl-CoA
pH 7.0, 30C
Pseudomonas aeruginosa
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
41000
-
x * 41000, SDS-PAGE, x * 42700, calculated
Pseudomonas aeruginosa
41000
-
x * 41000, SDS-PAGE, x * 42000, calculated
Pseudomonas aeruginosa
42000
-
x * 41000, SDS-PAGE, x * 42000, calculated
Pseudomonas aeruginosa
42700
-
x * 41000, SDS-PAGE, x * 42700, calculated
Pseudomonas aeruginosa
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant protein
Pseudomonas aeruginosa
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
citronellyl-CoA + 2,6-dichlorophenolindophenol
-
694044
Pseudomonas aeruginosa
cis-geranyl-CoA + reduced 2,6-dichlorophenolindophenol
-
-
-
?
additional information
no substrates: 5-methylhex-4-enoyl-CoA or isovaleryl-CoA
694044
Pseudomonas aeruginosa
?
-
-
-
-
additional information
no substrates: octanoyl-CoA, 5-methylhex-4-enoyl-CoA or isovaleryl-CoA
694044
Pseudomonas aeruginosa
?
-
-
-
-
octanoyl-CoA + 2,6-dichlorophenolindophenol
-
694044
Pseudomonas aeruginosa
cis-geranyl-CoA + reduced 2,6-dichlorophenolindophenol
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 41000, SDS-PAGE, x * 42700, calculated
Pseudomonas aeruginosa
?
x * 41000, SDS-PAGE, x * 42000, calculated
Pseudomonas aeruginosa
Other publictions for EC 1.3.99.B2
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
694044
Foerster-Fromme
Biochemical characterization o ...
Pseudomonas aeruginosa
Microbiology
154
789-796
2008
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