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Literature summary for 1.3.99.B2 extracted from

  • Foerster-Fromme, K.; Chattopadhyay, A.; Jendrossek, D.
    Biochemical characterization of AtuD from Pseudomonas aeruginosa, the first member of a new subgroup of acyl-CoA dehydrogenases with specificity for citronellyl-CoA (2008), Microbiology, 154, 789-796.
    View publication on PubMed
Search for more literature for 1.3.99.B2

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pseudomonas aeruginosa

Protein Variants

Protein Variants Comment Organism
additional information gene insertion mutant is not able to grow on citronellol, geraniol, citronellate, geranylate as sole carbon source Pseudomonas aeruginosa
additional information gene insertion mutant mutants grow slightly more slowly on citronellol and citronellate but show normal growth on geraniol and geranylate Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0016
-
 citronellyl-CoA pH 7.0, 30°C Pseudomonas aeruginosa
0.018
-
 citronellyl-CoA pH 7.0, 30°C Pseudomonas aeruginosa
0.13
-
 octanoyl-CoA pH 7.0, 30°C Pseudomonas aeruginosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41000
-
 x * 41000, SDS-PAGE, x * 42000, calculated Pseudomonas aeruginosa
41000
-
 x * 41000, SDS-PAGE, x * 42700, calculated Pseudomonas aeruginosa
42000
-
 x * 41000, SDS-PAGE, x * 42000, calculated Pseudomonas aeruginosa
42700
-
 x * 41000, SDS-PAGE, x * 42700, calculated Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa Q9HZV8 gene atud; specific expression in cells growing on acyclic terpenes, absent in isovalerate- or succinate-grown cells
-
Pseudomonas aeruginosa Q9I3H8 gene PA1535, second citronellyl-CoA dehydrogenase, less specific; specific expression in cells growing on acyclic terpenes, absent in isovalerate- or succinate-grown cells
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
citronellyl-CoA + 2,6-dichlorophenolindophenol
-
 Pseudomonas aeruginosa cis-geranyl-CoA + reduced 2,6-dichlorophenolindophenol
-
 ?
additional information no substrates: 5-methylhex-4-enoyl-CoA or isovaleryl-CoA Pseudomonas aeruginosa ?
-
 ?
additional information no substrates: octanoyl-CoA, 5-methylhex-4-enoyl-CoA or isovaleryl-CoA Pseudomonas aeruginosa ?
-
 ?
octanoyl-CoA + 2,6-dichlorophenolindophenol
-
 Pseudomonas aeruginosa cis-geranyl-CoA + reduced 2,6-dichlorophenolindophenol
-
 ?

Subunits

Subunits Comment Organism
? x * 41000, SDS-PAGE, x * 42000, calculated Pseudomonas aeruginosa
? x * 41000, SDS-PAGE, x * 42700, calculated Pseudomonas aeruginosa

Cofactor

Cofactor Comment Organism Structure
FAD purified and concentrated protein is yellow, and spectral analysis confirms the presence of FAD as cofactor Pseudomonas aeruginosa