Literature summary for 1.3.99.5 extracted from

  • Florin, C.; Koehler, T.; Grandguillot, M.; Plesiat, P.
    Comamonas testosteroni 3-ketosteroid-DELTA4(5alpha)-dehydrogenase (1996), J. Bacteriol., 178, 3322-3330.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli JM109 carrying pXE25, activity is maximal 18times higher than the activity expressed by induced cells of Comamonas testosteroni. Overexpression in Pseudomonas putida KT2440 using pMMB208 as expression vector, activity is maximaly 264times higher than the activity expressed by induced cells of Comamonas testosteroni Comamonas testosteroni

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0161
-
5alpha-androstane-3,17-dione reaction with N,N,N‘,N‘-tetramethyl-1,4-phenylenediamine Comamonas testosteroni
0.0066
-
1-Androstene-3,17-dione reaction with N,N,N‘,N‘-tetramethyl-1,4-phenylenediamine Comamonas testosteroni

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
x * 60000, SDS-PAGE Comamonas testosteroni
58100
-
x * 58100, calculation from nucleotide sequence Comamonas testosteroni

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
testosterone + reduced acceptor Comamonas testosteroni key enzyme in the degradation of steroids having an A:B ring fusion in a trans configuration 5alpha-dihydrotestosterone + acceptor
-
?

Organism

Organism UniProt Comment Textmining
Comamonas testosteroni
-
formerly Pseudomonas testosteroni
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
testosterone + reduced acceptor key enzyme in the degradation of steroids having an A:B ring fusion in a trans configuration Comamonas testosteroni 5alpha-dihydrotestosterone + acceptor
-
?
1-androstene-3,17-dione + phenazine methosulfate acceptor: N,N,N‘,N‘-tetramethyl-1,4-phenylenediamine, menadione, phenazine methosulfate, cytochrome c Comamonas testosteroni 1,4-androstadiene-3,17-dione + reduced phenazine methosulfate
-
ir
5alpha-1-androsten-3,17-dione + acceptor acceptor: N,N,N‘,N‘-tetramethyl-1,4-phenylenediamine, menadione, phenazine methosulfate, cytochrome c Comamonas testosteroni ? + reduced acceptor
-
ir
5alpha-androstane-3,17-dione + N,N,N',N'-tetramethyl-1,4-phenylendiamine
-
Comamonas testosteroni androst-4-ene-3,17-dione + reduced N,N,N',N'-tetramethyl-1,4-phenylendiamine
-
r

Subunits

Subunits Comment Organism
? x * 60000, SDS-PAGE Comamonas testosteroni
? x * 58100, calculation from nucleotide sequence Comamonas testosteroni

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
reaction with 1-androstene-3,17-dione and N,N,N‘,N‘-tetramethyl-1,4-phenylenediamine Comamonas testosteroni

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 50 25°C: about 50% of maximal activity, 50°C: about 20% of maximal activity, reaction with N,N,N‘,N‘-tetramethyl-1,4-phenylenediamine Comamonas testosteroni

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 9 reaction with 1-androstene-3,17-dione and N,N,N‘,N‘-tetramethyl-1,4-phenylenediamine Comamonas testosteroni

pH Range

pH Minimum pH Maximum Comment Organism
7 9 pH 7.0: about 40% of maximal activity, pH 8.0-9.0: maximal activity, reaction with Delta1-androstene-3,17-dione and N,N,N‘,N‘-tetramethyl-1,4-phenylenediamine Comamonas testosteroni