Literature summary for 1.3.99.41 extracted from

Reisch, C.R.; Stoudemayer, M.J.; Varaljay, V.A.; Amster, I.J.; Moran, M.A.; Whitman, W.B.
Novel pathway for assimilation of dimethylsulphoniopropionate widespread in marine bacteria (2011), Nature, 473, 208-211.

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Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
62854	-	x * 62854, calculated from sequence	Ruegeria pomeroyi

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
3-(methylthio)propionyl-CoA + acceptor	Ruegeria pomeroyi	once released from phytoplankton, marine bacteria degrade dimethylsulfoniopropionate by either the cleavage pathway (to form the volatile gas dimethylsulfide) or the demethylation pathway (yielding methanethiol, which is readily assimilated or oxidized). The enzyme DmdC, 3-(methylthio)propionyl-CoA dehydrogenase, catalyzes the first step in the demethylation pathway	(2E)-3-(methylthio)prop-2-enoyl-CoA + reduced acceptor	-	?

Organism

Organism	UniProt	Comment	Textmining
Ruegeria pomeroyi	Q5LLW7	-	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
1.23	-	chemostat, pH and temperature not specified in the publication	Ruegeria pomeroyi

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3-(methylthio)propionyl-CoA + acceptor	-	Ruegeria pomeroyi	(2E)-3-(methylthio)prop-2-enoyl-CoA + reduced acceptor	-	?
3-(methylthio)propionyl-CoA + acceptor	once released from phytoplankton, marine bacteria degrade dimethylsulfoniopropionate by either the cleavage pathway (to form the volatile gas dimethylsulfide) or the demethylation pathway (yielding methanethiol, which is readily assimilated or oxidized). The enzyme DmdC, 3-(methylthio)propionyl-CoA dehydrogenase, catalyzes the first step in the demethylation pathway	Ruegeria pomeroyi	(2E)-3-(methylthio)prop-2-enoyl-CoA + reduced acceptor	-	?

Subunits

Subunits	Comment	Organism
?	x * 62854, calculated from sequence	Ruegeria pomeroyi

Synonyms

Synonyms	Comment	Organism
3-methylmercaptopropionyl-CoA dehydrogenase	-	Ruegeria pomeroyi
dmdC	-	Ruegeria pomeroyi
SPO3804	locus name	Ruegeria pomeroyi

Expression

Organism	Comment	Expression
Ruegeria pomeroyi	the amount of transcripts for dmdB, dmdC and dmdD increases during growth of wild-type enzyme on methylmercaptopropionate or 3-(dimethylsulphonio)propanoate, as expected if the pathway is required for 3-(methylthio)propanoate metabolism. Following growth with 3-(dimethylsulphonio)propanoate, the levels of DmdC and DmdD activity in the deletion mutant dmdC are greatly reduced	up

General Information

General Information	Comment	Organism
malfunction	a dmdC2 mutant (SPO3804::Tn5) can not grow on 3-(methylthio)propanoate as the sole source of carbon, indicating that this pathway is essential for growth on 3-(methylthio)propanoate. In contrast, the mutant grows similarly to wild-type with 3-(dimethylsulphonio)propanoate, indicating that the cleavage pathway, initiated by DddQ or DddP13, remains capable of supporting growth	Ruegeria pomeroyi
metabolism	in extracts of chemostat-grown cells the levels of DmdB, DmdC and DmdD activities exceed the minimum level, 57 nmol/min*mg of protein, necessary to support growth. The amount of transcripts for dmdB, dmdC and dmdD increase during growth on 3-(methylthio)propanoate or 3-(dimethylsulphonio)propanoate, as expected if the pathway is required for 3-(methylthio)propanoate metabolism	Ruegeria pomeroyi

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Release 2023.1 (January 2023)