BRENDA - Enzyme Database
show all sequences of 1.3.99.4

3-Keto-5alpha-steroid DELTA(1)-dehydrogenase from Rhodococcus erythropolis SQ1 and its orthologue in Mycobacterium tuberculosis H37Rv are highly specific enzymes that function in cholesterol catabolism

Knol, J.; Bodewits, K.; Hessels, G.I.; Dijkhuizen, L.; van der Geize, R.; Biochem. J. 410, 339-346 (2008)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expressed in Escherichia coli BL21(DE3) cells; expressed in Escherichia coli BL21(DE3) cells; expressed in Escherichia coli BL21(DE3) cells
Rhodococcus erythropolis
General Stability
General Stability
Organism
KSTD3 activity is lost upon purification using Ni-NTA column chromatography
Rhodococcus erythropolis
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.003
-
progesterone
apparent value, in 50 mM Tris (pH 7.4), at 30C
Rhodococcus erythropolis
0.005
-
4-androstene-3,17-dione
apparent value, in 50 mM Tris (pH 7.4), at 30C
Rhodococcus erythropolis
0.006
-
5alpha-androstan-3-one-17beta-ol
apparent value, in 50 mM Tris (pH 7.4), at 30C
Rhodococcus erythropolis
0.007
-
5alpha-pregnane-3,20-dione
apparent value, in 50 mM Tris (pH 7.4), at 30C
Rhodococcus erythropolis
0.013
-
5alpha-androstane-3,17-dione
apparent value, in 50 mM Tris (pH 7.4), at 30C
Rhodococcus erythropolis
0.014
-
5alpha-pregnane-3,20-dione
apparent value, in 50 mM Tris (pH 7.4), at 30C
Rhodococcus erythropolis
0.018
-
progesterone
apparent value, in 50 mM Tris (pH 7.4), at 30C
Rhodococcus erythropolis
0.021
-
5alpha-pregnane-3,20-dione
apparent value, in 50 mM Tris (pH 7.4), at 30C
Rhodococcus erythropolis
0.026
-
23,24-bis-nor-5alpha-cholestan-3-one acid
apparent value, in 50 mM Tris (pH 7.4), at 30C
Rhodococcus erythropolis
0.029
-
progesterone
apparent value, in 50 mM Tris (pH 7.4), at 30C
Rhodococcus erythropolis
0.033
-
5alpha-androstane-3,17-dione
apparent value, in 50 mM Tris (pH 7.4), at 30C
Mycobacterium tuberculosis
0.036
-
5alpha-androstan-3-one-17beta-ol
apparent value, in 50 mM Tris (pH 7.4), at 30C
Rhodococcus erythropolis
0.036
-
5alpha-androstane-3,17-dione
apparent value, in 50 mM Tris (pH 7.4), at 30C
Rhodococcus erythropolis
0.058
-
9alpha-hydroxy-4-androstene-3,17-dione
apparent value, in 50 mM Tris (pH 7.4), at 30C
Rhodococcus erythropolis
0.059
-
5alpha-androstane-3,17-dione
apparent value, in 50 mM Tris (pH 7.4), at 30C
Rhodococcus erythropolis
0.064
-
11beta-cortisol
apparent value, in 50 mM Tris (pH 7.4), at 30C
Rhodococcus erythropolis
0.065
-
progesterone
apparent value, in 50 mM Tris (pH 7.4), at 30C
Mycobacterium tuberculosis
0.07
-
5alpha-androstan-3-one-17beta-ol
apparent value, in 50 mM Tris (pH 7.4), at 30C
Rhodococcus erythropolis
0.074
-
23,24-bis-nor-5alpha-cholestan-3-one acid
apparent value, in 50 mM Tris (pH 7.4), at 30C
Rhodococcus erythropolis
0.104
-
4-androstene-3,17-dione
apparent value, in 50 mM Tris (pH 7.4), at 30C
Rhodococcus erythropolis
0.119
-
9alpha-hydroxy-4-androstene-3,17-dione
apparent value, in 50 mM Tris (pH 7.4), at 30C
Rhodococcus erythropolis
0.166
-
5alpha-androstan-3-one-17beta-ol
apparent value, in 50 mM Tris (pH 7.4), at 30C
Mycobacterium tuberculosis
0.557
-
11beta-cortisol
apparent value, in 50 mM Tris (pH 7.4), at 30C
Rhodococcus erythropolis
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
53000
-
SDS-PAGE
Rhodococcus erythropolis
60000
-
SDS-PAGE
Rhodococcus erythropolis
61000
-
SDS-PAGE
Rhodococcus erythropolis
Organism
Organism
UniProt
Commentary
Textmining
Mycobacterium tuberculosis
-
-
-
Mycobacterium tuberculosis H37Rv
-
-
-
Rhodococcus erythropolis
A9QAE5
isozyme KSTD3
-
Rhodococcus erythropolis
Q8RLT5
isozyme KSTD2
-
Rhodococcus erythropolis
Q9RA02
isozyme KSTD1
-
Rhodococcus erythropolis SQ1
A9QAE5
isozyme KSTD3
-
Rhodococcus erythropolis SQ1
Q8RLT5
isozyme KSTD2
-
Rhodococcus erythropolis SQ1
Q9RA02
isozyme KSTD1
-
Purification (Commentary)
Purification (Commentary)
Organism
KSTD3 activity is lost upon purification using Ni-NTA column chromatography
Rhodococcus erythropolis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
11beta-cortisol + 2,6-dichlorophenol-indophenol
-
684994
Rhodococcus erythropolis
(11beta)-11,17,21-trihydroxypregna-1,4-diene-3,20-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
11beta-cortisol + 2,6-dichlorophenol-indophenol
-
684994
Rhodococcus erythropolis SQ1
(11beta)-11,17,21-trihydroxypregna-1,4-diene-3,20-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
23,24-bis-nor-5alpha-cholestan-3-one acid + 2,6-dichlorophenol-indophenol
-
684994
Rhodococcus erythropolis
?
-
-
-
?
23,24-bis-nor-5alpha-cholestan-3-one acid + 2,6-dichlorophenol-indophenol
-
684994
Rhodococcus erythropolis SQ1
?
-
-
-
?
4-androstene-3,17-dione + 2,6-dichlorophenol-indophenol
-
684994
Rhodococcus erythropolis
1,4-androstene-3,17-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
4-androstene-3,17-dione + 2,6-dichlorophenol-indophenol
-
684994
Rhodococcus erythropolis SQ1
1,4-androstene-3,17-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
4-pregnene-3,20-dione + acceptor
-
684994
Rhodococcus erythropolis
1,4-pregnene-3,20 dione + reduced acceptor
-
-
-
-
4-pregnene-3,20-dione + acceptor
-
684994
Rhodococcus erythropolis SQ1
1,4-pregnene-3,20 dione + reduced acceptor
-
-
-
-
5alpha-androstan-3-one-17beta-ol + 2,6-dichlorophenol-indophenol
-
684994
Rhodococcus erythropolis
(5alpha,17beta)-17-hydroxyandrost-1-en-3-one + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
5alpha-androstan-3-one-17beta-ol + 2,6-dichlorophenol-indophenol
KSTD3 shows highest activity on 5alpha-androstan-3-one-17beta-ol which is also known as 17beta-hydroxy-5alpha-androstane-3-one
684994
Rhodococcus erythropolis
(5alpha,17beta)-17-hydroxyandrost-1-en-3-one + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
5alpha-androstan-3-one-17beta-ol + 2,6-dichlorophenol-indophenol
KSTD3 shows highest activity on 5alpha-testosterone which is also known as 17beta-hydroxy-5alpha-androstane-3-one
684994
Mycobacterium tuberculosis
(5alpha,17beta)-17-hydroxyandrost-1-en-3-one + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
5alpha-androstan-3-one-17beta-ol + 2,6-dichlorophenol-indophenol
KSTD3 shows highest activity on 5alpha-androstan-3-one-17beta-ol which is also known as 17beta-hydroxy-5alpha-androstane-3-one
684994
Rhodococcus erythropolis SQ1
(5alpha,17beta)-17-hydroxyandrost-1-en-3-one + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
5alpha-androstan-3-one-17beta-ol + 2,6-dichlorophenol-indophenol
-
684994
Rhodococcus erythropolis SQ1
(5alpha,17beta)-17-hydroxyandrost-1-en-3-one + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
5alpha-androstan-3-one-17beta-ol + 2,6-dichlorophenol-indophenol
KSTD3 shows highest activity on 5alpha-testosterone which is also known as 17beta-hydroxy-5alpha-androstane-3-one
684994
Mycobacterium tuberculosis H37Rv
(5alpha,17beta)-17-hydroxyandrost-1-en-3-one + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
5alpha-androstane-3,17-dione + 2,6-dichlorophenol-indophenol
-
684994
Rhodococcus erythropolis
5beta-androst-1-en-3,17-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
5alpha-androstane-3,17-dione + 2,6-dichlorophenol-indophenol
KSTD3 shows highest activity with 5alpha-androstane-3,17-dione
684994
Mycobacterium tuberculosis
5beta-androst-1-en-3,17-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
5alpha-androstane-3,17-dione + 2,6-dichlorophenol-indophenol
KSTD3 shows highest activity with 5alpha-androstane-3,17-dione
684994
Rhodococcus erythropolis
5beta-androst-1-en-3,17-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
5alpha-androstane-3,17-dione + 2,6-dichlorophenol-indophenol
KSTD3 shows highest activity with 5alpha-androstane-3,17-dione
684994
Rhodococcus erythropolis SQ1
5beta-androst-1-en-3,17-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
5alpha-androstane-3,17-dione + 2,6-dichlorophenol-indophenol
KSTD3 shows highest activity with 5alpha-androstane-3,17-dione
684994
Mycobacterium tuberculosis H37Rv
5beta-androst-1-en-3,17-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
5alpha-pregnane-3,20-dione + 2,6-dichlorophenol-indophenol
-
684994
Rhodococcus erythropolis
5alpha-pregn-1-en-3,20-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
5alpha-pregnane-3,20-dione + 2,6-dichlorophenol-indophenol
-
684994
Rhodococcus erythropolis SQ1
5alpha-pregn-1-en-3,20-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
9alpha-hydroxy-4-androstene-3,17-dione + 2,6-dichlorophenol-indophenol
-
684994
Rhodococcus erythropolis
9alpha-hydroxy-1,4-androstadiene 3,17-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
9alpha-hydroxy-4-androstene-3,17-dione + 2,6-dichlorophenol-indophenol
preferred substrate of isozyme KSTD2
684994
Rhodococcus erythropolis
9alpha-hydroxy-1,4-androstadiene 3,17-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
9alpha-hydroxy-4-androstene-3,17-dione + 2,6-dichlorophenol-indophenol
-
684994
Rhodococcus erythropolis
9alpha-hydroxy-androst-1,4-diene-3,17-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
additional information
4-androstene-3,17-dione, 9alpha-hydroxy-4-androstene-3,17-dione, 11beta-cortisol, 23,24-bis-nor-5alpha-cholestan-3-one acid, 5beta-androstane-3,17-dione, 1-(5alpha)-androstene-3,17-dione, 11alpha-hydroxy-4-norandrostene-3,17-dione, 1,4-androstadiene-3,17-dione, dehydroisoandrosterone, 4-cholesten-3-one, 5alpha-cholestan-3-one, and 5alpha-androstan-3beta-ol,17-one are no substrates
684994
Rhodococcus erythropolis
?
-
-
-
-
additional information
4-androstene-3,17-dione, 9alpha-hydroxy-4-androstene-3,17-dione, 11beta-cortisol, 23,24-bisnor-5alpha-cholestan-3-one acid, 5beta-androstane-3,17-dione, [1-(5alpha)-androstene-3,17-dione], 11alpha-hydroxy-4-norandrostene-3,17-dione, 1,4-androstadiene-3,17-dione, dehydroisoandrosterone, 5alpha-pregnane-3,20-dione, 4-cholesten-3-one, 5alpha-cholestan-3-one, and 5alpha-androstan-3beta-ol, 17-one are no substrates
684994
Mycobacterium tuberculosis
?
-
-
-
-
additional information
5beta-androstane-3,17-dione, 1-(5alpha)-androstene-3,17-dione, 11alpha-hydroxy-4-norandrostene-3,17-dione, 1,4-androstadiene-3,17-dione, dehydroisoandrosterone, 4-cholesten-3-one, 5alpha-cholestan-3-one, and 5alpha-androstan-3beta-ol,17-one are no substrates
684994
Rhodococcus erythropolis
?
-
-
-
-
additional information
5beta-androstane-3,17-dione, [1-(5alpha)-androstene-3,17-dione], 11alpha-hydroxy-4-norandrostene-3,17-dione, 1,4-androstadiene-3,17-dione, dehydroisoandrosterone, 4-cholesten-3-one, 5alpha-cholestan-3-one, and 5alpha-androstan-3beta-ol,17-one are no substrates
684994
Rhodococcus erythropolis
?
-
-
-
-
additional information
4-androstene-3,17-dione, 9alpha-hydroxy-4-androstene-3,17-dione, 11beta-cortisol, 23,24-bis-nor-5alpha-cholestan-3-one acid, 5beta-androstane-3,17-dione, 1-(5alpha)-androstene-3,17-dione, 11alpha-hydroxy-4-norandrostene-3,17-dione, 1,4-androstadiene-3,17-dione, dehydroisoandrosterone, 4-cholesten-3-one, 5alpha-cholestan-3-one, and 5alpha-androstan-3beta-ol,17-one are no substrates
684994
Rhodococcus erythropolis SQ1
?
-
-
-
-
additional information
5beta-androstane-3,17-dione, [1-(5alpha)-androstene-3,17-dione], 11alpha-hydroxy-4-norandrostene-3,17-dione, 1,4-androstadiene-3,17-dione, dehydroisoandrosterone, 4-cholesten-3-one, 5alpha-cholestan-3-one, and 5alpha-androstan-3beta-ol,17-one are no substrates
684994
Rhodococcus erythropolis SQ1
?
-
-
-
-
additional information
5beta-androstane-3,17-dione, 1-(5alpha)-androstene-3,17-dione, 11alpha-hydroxy-4-norandrostene-3,17-dione, 1,4-androstadiene-3,17-dione, dehydroisoandrosterone, 4-cholesten-3-one, 5alpha-cholestan-3-one, and 5alpha-androstan-3beta-ol,17-one are no substrates
684994
Rhodococcus erythropolis SQ1
?
-
-
-
-
additional information
4-androstene-3,17-dione, 9alpha-hydroxy-4-androstene-3,17-dione, 11beta-cortisol, 23,24-bisnor-5alpha-cholestan-3-one acid, 5beta-androstane-3,17-dione, [1-(5alpha)-androstene-3,17-dione], 11alpha-hydroxy-4-norandrostene-3,17-dione, 1,4-androstadiene-3,17-dione, dehydroisoandrosterone, 5alpha-pregnane-3,20-dione, 4-cholesten-3-one, 5alpha-cholestan-3-one, and 5alpha-androstan-3beta-ol, 17-one are no substrates
684994
Mycobacterium tuberculosis H37Rv
?
-
-
-
-
progesterone + 2,6-dichlorophenol-indophenol
-
684994
Mycobacterium tuberculosis
pregna-1,4-diene-3,20-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
progesterone + 2,6-dichlorophenol-indophenol
-
684994
Rhodococcus erythropolis
pregna-1,4-diene-3,20-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
progesterone + 2,6-dichlorophenol-indophenol
-
684994
Mycobacterium tuberculosis H37Rv
pregna-1,4-diene-3,20-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
3-keto-5alpha-steroid DELTA(1)-dehydrogenase
-
Mycobacterium tuberculosis
3-keto-5alpha-steroid DELTA(1)-dehydrogenase
-
Rhodococcus erythropolis
4-ene-3-oxosteroid: (acceptor)-1-ene-oxoreductase
-
Mycobacterium tuberculosis
4-ene-3-oxosteroid: (acceptor)-1-ene-oxoreductase
-
Rhodococcus erythropolis
KSTD1
isozyme
Rhodococcus erythropolis
KSTD2
isozyme
Rhodococcus erythropolis
KSTD3
isozyme
Rhodococcus erythropolis
KSTD3 orthologue
-
Mycobacterium tuberculosis
Rv3537
-
Mycobacterium tuberculosis
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Rhodococcus erythropolis
General Stability (protein specific)
General Stability
Organism
KSTD3 activity is lost upon purification using Ni-NTA column chromatography
Rhodococcus erythropolis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.003
-
progesterone
apparent value, in 50 mM Tris (pH 7.4), at 30C
Rhodococcus erythropolis
0.005
-
4-androstene-3,17-dione
apparent value, in 50 mM Tris (pH 7.4), at 30C
Rhodococcus erythropolis
0.006
-
5alpha-androstan-3-one-17beta-ol
apparent value, in 50 mM Tris (pH 7.4), at 30C
Rhodococcus erythropolis
0.007
-
5alpha-pregnane-3,20-dione
apparent value, in 50 mM Tris (pH 7.4), at 30C
Rhodococcus erythropolis
0.013
-
5alpha-androstane-3,17-dione
apparent value, in 50 mM Tris (pH 7.4), at 30C
Rhodococcus erythropolis
0.014
-
5alpha-pregnane-3,20-dione
apparent value, in 50 mM Tris (pH 7.4), at 30C
Rhodococcus erythropolis
0.018
-
progesterone
apparent value, in 50 mM Tris (pH 7.4), at 30C
Rhodococcus erythropolis
0.021
-
5alpha-pregnane-3,20-dione
apparent value, in 50 mM Tris (pH 7.4), at 30C
Rhodococcus erythropolis
0.026
-
23,24-bis-nor-5alpha-cholestan-3-one acid
apparent value, in 50 mM Tris (pH 7.4), at 30C
Rhodococcus erythropolis
0.029
-
progesterone
apparent value, in 50 mM Tris (pH 7.4), at 30C
Rhodococcus erythropolis
0.033
-
5alpha-androstane-3,17-dione
apparent value, in 50 mM Tris (pH 7.4), at 30C
Mycobacterium tuberculosis
0.036
-
5alpha-androstan-3-one-17beta-ol
apparent value, in 50 mM Tris (pH 7.4), at 30C
Rhodococcus erythropolis
0.036
-
5alpha-androstane-3,17-dione
apparent value, in 50 mM Tris (pH 7.4), at 30C
Rhodococcus erythropolis
0.058
-
9alpha-hydroxy-4-androstene-3,17-dione
apparent value, in 50 mM Tris (pH 7.4), at 30C
Rhodococcus erythropolis
0.059
-
5alpha-androstane-3,17-dione
apparent value, in 50 mM Tris (pH 7.4), at 30C
Rhodococcus erythropolis
0.064
-
11beta-cortisol
apparent value, in 50 mM Tris (pH 7.4), at 30C
Rhodococcus erythropolis
0.065
-
progesterone
apparent value, in 50 mM Tris (pH 7.4), at 30C
Mycobacterium tuberculosis
0.07
-
5alpha-androstan-3-one-17beta-ol
apparent value, in 50 mM Tris (pH 7.4), at 30C
Rhodococcus erythropolis
0.074
-
23,24-bis-nor-5alpha-cholestan-3-one acid
apparent value, in 50 mM Tris (pH 7.4), at 30C
Rhodococcus erythropolis
0.104
-
4-androstene-3,17-dione
apparent value, in 50 mM Tris (pH 7.4), at 30C
Rhodococcus erythropolis
0.119
-
9alpha-hydroxy-4-androstene-3,17-dione
apparent value, in 50 mM Tris (pH 7.4), at 30C
Rhodococcus erythropolis
0.166
-
5alpha-androstan-3-one-17beta-ol
apparent value, in 50 mM Tris (pH 7.4), at 30C
Mycobacterium tuberculosis
0.557
-
11beta-cortisol
apparent value, in 50 mM Tris (pH 7.4), at 30C
Rhodococcus erythropolis
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
53000
-
SDS-PAGE
Rhodococcus erythropolis
60000
-
SDS-PAGE
Rhodococcus erythropolis
61000
-
SDS-PAGE
Rhodococcus erythropolis
Purification (Commentary) (protein specific)
Commentary
Organism
KSTD3 activity is lost upon purification using Ni-NTA column chromatography
Rhodococcus erythropolis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
11beta-cortisol + 2,6-dichlorophenol-indophenol
-
684994
Rhodococcus erythropolis
(11beta)-11,17,21-trihydroxypregna-1,4-diene-3,20-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
11beta-cortisol + 2,6-dichlorophenol-indophenol
-
684994
Rhodococcus erythropolis SQ1
(11beta)-11,17,21-trihydroxypregna-1,4-diene-3,20-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
23,24-bis-nor-5alpha-cholestan-3-one acid + 2,6-dichlorophenol-indophenol
-
684994
Rhodococcus erythropolis
?
-
-
-
?
23,24-bis-nor-5alpha-cholestan-3-one acid + 2,6-dichlorophenol-indophenol
-
684994
Rhodococcus erythropolis SQ1
?
-
-
-
?
4-androstene-3,17-dione + 2,6-dichlorophenol-indophenol
-
684994
Rhodococcus erythropolis
1,4-androstene-3,17-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
4-androstene-3,17-dione + 2,6-dichlorophenol-indophenol
-
684994
Rhodococcus erythropolis SQ1
1,4-androstene-3,17-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
4-pregnene-3,20-dione + acceptor
-
684994
Rhodococcus erythropolis
1,4-pregnene-3,20 dione + reduced acceptor
-
-
-
-
4-pregnene-3,20-dione + acceptor
-
684994
Rhodococcus erythropolis SQ1
1,4-pregnene-3,20 dione + reduced acceptor
-
-
-
-
5alpha-androstan-3-one-17beta-ol + 2,6-dichlorophenol-indophenol
-
684994
Rhodococcus erythropolis
(5alpha,17beta)-17-hydroxyandrost-1-en-3-one + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
5alpha-androstan-3-one-17beta-ol + 2,6-dichlorophenol-indophenol
KSTD3 shows highest activity on 5alpha-androstan-3-one-17beta-ol which is also known as 17beta-hydroxy-5alpha-androstane-3-one
684994
Rhodococcus erythropolis
(5alpha,17beta)-17-hydroxyandrost-1-en-3-one + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
5alpha-androstan-3-one-17beta-ol + 2,6-dichlorophenol-indophenol
KSTD3 shows highest activity on 5alpha-testosterone which is also known as 17beta-hydroxy-5alpha-androstane-3-one
684994
Mycobacterium tuberculosis
(5alpha,17beta)-17-hydroxyandrost-1-en-3-one + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
5alpha-androstan-3-one-17beta-ol + 2,6-dichlorophenol-indophenol
KSTD3 shows highest activity on 5alpha-androstan-3-one-17beta-ol which is also known as 17beta-hydroxy-5alpha-androstane-3-one
684994
Rhodococcus erythropolis SQ1
(5alpha,17beta)-17-hydroxyandrost-1-en-3-one + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
5alpha-androstan-3-one-17beta-ol + 2,6-dichlorophenol-indophenol
-
684994
Rhodococcus erythropolis SQ1
(5alpha,17beta)-17-hydroxyandrost-1-en-3-one + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
5alpha-androstan-3-one-17beta-ol + 2,6-dichlorophenol-indophenol
KSTD3 shows highest activity on 5alpha-testosterone which is also known as 17beta-hydroxy-5alpha-androstane-3-one
684994
Mycobacterium tuberculosis H37Rv
(5alpha,17beta)-17-hydroxyandrost-1-en-3-one + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
5alpha-androstane-3,17-dione + 2,6-dichlorophenol-indophenol
-
684994
Rhodococcus erythropolis
5beta-androst-1-en-3,17-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
5alpha-androstane-3,17-dione + 2,6-dichlorophenol-indophenol
KSTD3 shows highest activity with 5alpha-androstane-3,17-dione
684994
Mycobacterium tuberculosis
5beta-androst-1-en-3,17-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
5alpha-androstane-3,17-dione + 2,6-dichlorophenol-indophenol
KSTD3 shows highest activity with 5alpha-androstane-3,17-dione
684994
Rhodococcus erythropolis
5beta-androst-1-en-3,17-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
5alpha-androstane-3,17-dione + 2,6-dichlorophenol-indophenol
KSTD3 shows highest activity with 5alpha-androstane-3,17-dione
684994
Rhodococcus erythropolis SQ1
5beta-androst-1-en-3,17-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
5alpha-androstane-3,17-dione + 2,6-dichlorophenol-indophenol
KSTD3 shows highest activity with 5alpha-androstane-3,17-dione
684994
Mycobacterium tuberculosis H37Rv
5beta-androst-1-en-3,17-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
5alpha-pregnane-3,20-dione + 2,6-dichlorophenol-indophenol
-
684994
Rhodococcus erythropolis
5alpha-pregn-1-en-3,20-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
5alpha-pregnane-3,20-dione + 2,6-dichlorophenol-indophenol
-
684994
Rhodococcus erythropolis SQ1
5alpha-pregn-1-en-3,20-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
9alpha-hydroxy-4-androstene-3,17-dione + 2,6-dichlorophenol-indophenol
-
684994
Rhodococcus erythropolis
9alpha-hydroxy-1,4-androstadiene 3,17-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
9alpha-hydroxy-4-androstene-3,17-dione + 2,6-dichlorophenol-indophenol
preferred substrate of isozyme KSTD2
684994
Rhodococcus erythropolis
9alpha-hydroxy-1,4-androstadiene 3,17-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
9alpha-hydroxy-4-androstene-3,17-dione + 2,6-dichlorophenol-indophenol
-
684994
Rhodococcus erythropolis
9alpha-hydroxy-androst-1,4-diene-3,17-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
additional information
4-androstene-3,17-dione, 9alpha-hydroxy-4-androstene-3,17-dione, 11beta-cortisol, 23,24-bis-nor-5alpha-cholestan-3-one acid, 5beta-androstane-3,17-dione, 1-(5alpha)-androstene-3,17-dione, 11alpha-hydroxy-4-norandrostene-3,17-dione, 1,4-androstadiene-3,17-dione, dehydroisoandrosterone, 4-cholesten-3-one, 5alpha-cholestan-3-one, and 5alpha-androstan-3beta-ol,17-one are no substrates
684994
Rhodococcus erythropolis
?
-
-
-
-
additional information
4-androstene-3,17-dione, 9alpha-hydroxy-4-androstene-3,17-dione, 11beta-cortisol, 23,24-bisnor-5alpha-cholestan-3-one acid, 5beta-androstane-3,17-dione, [1-(5alpha)-androstene-3,17-dione], 11alpha-hydroxy-4-norandrostene-3,17-dione, 1,4-androstadiene-3,17-dione, dehydroisoandrosterone, 5alpha-pregnane-3,20-dione, 4-cholesten-3-one, 5alpha-cholestan-3-one, and 5alpha-androstan-3beta-ol, 17-one are no substrates
684994
Mycobacterium tuberculosis
?
-
-
-
-
additional information
5beta-androstane-3,17-dione, 1-(5alpha)-androstene-3,17-dione, 11alpha-hydroxy-4-norandrostene-3,17-dione, 1,4-androstadiene-3,17-dione, dehydroisoandrosterone, 4-cholesten-3-one, 5alpha-cholestan-3-one, and 5alpha-androstan-3beta-ol,17-one are no substrates
684994
Rhodococcus erythropolis
?
-
-
-
-
additional information
5beta-androstane-3,17-dione, [1-(5alpha)-androstene-3,17-dione], 11alpha-hydroxy-4-norandrostene-3,17-dione, 1,4-androstadiene-3,17-dione, dehydroisoandrosterone, 4-cholesten-3-one, 5alpha-cholestan-3-one, and 5alpha-androstan-3beta-ol,17-one are no substrates
684994
Rhodococcus erythropolis
?
-
-
-
-
additional information
4-androstene-3,17-dione, 9alpha-hydroxy-4-androstene-3,17-dione, 11beta-cortisol, 23,24-bis-nor-5alpha-cholestan-3-one acid, 5beta-androstane-3,17-dione, 1-(5alpha)-androstene-3,17-dione, 11alpha-hydroxy-4-norandrostene-3,17-dione, 1,4-androstadiene-3,17-dione, dehydroisoandrosterone, 4-cholesten-3-one, 5alpha-cholestan-3-one, and 5alpha-androstan-3beta-ol,17-one are no substrates
684994
Rhodococcus erythropolis SQ1
?
-
-
-
-
additional information
5beta-androstane-3,17-dione, [1-(5alpha)-androstene-3,17-dione], 11alpha-hydroxy-4-norandrostene-3,17-dione, 1,4-androstadiene-3,17-dione, dehydroisoandrosterone, 4-cholesten-3-one, 5alpha-cholestan-3-one, and 5alpha-androstan-3beta-ol,17-one are no substrates
684994
Rhodococcus erythropolis SQ1
?
-
-
-
-
additional information
5beta-androstane-3,17-dione, 1-(5alpha)-androstene-3,17-dione, 11alpha-hydroxy-4-norandrostene-3,17-dione, 1,4-androstadiene-3,17-dione, dehydroisoandrosterone, 4-cholesten-3-one, 5alpha-cholestan-3-one, and 5alpha-androstan-3beta-ol,17-one are no substrates
684994
Rhodococcus erythropolis SQ1
?
-
-
-
-
additional information
4-androstene-3,17-dione, 9alpha-hydroxy-4-androstene-3,17-dione, 11beta-cortisol, 23,24-bisnor-5alpha-cholestan-3-one acid, 5beta-androstane-3,17-dione, [1-(5alpha)-androstene-3,17-dione], 11alpha-hydroxy-4-norandrostene-3,17-dione, 1,4-androstadiene-3,17-dione, dehydroisoandrosterone, 5alpha-pregnane-3,20-dione, 4-cholesten-3-one, 5alpha-cholestan-3-one, and 5alpha-androstan-3beta-ol, 17-one are no substrates
684994
Mycobacterium tuberculosis H37Rv
?
-
-
-
-
progesterone + 2,6-dichlorophenol-indophenol
-
684994
Mycobacterium tuberculosis
pregna-1,4-diene-3,20-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
progesterone + 2,6-dichlorophenol-indophenol
-
684994
Rhodococcus erythropolis
pregna-1,4-diene-3,20-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
progesterone + 2,6-dichlorophenol-indophenol
-
684994
Mycobacterium tuberculosis H37Rv
pregna-1,4-diene-3,20-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
Other publictions for EC 1.3.99.4
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
743236
Guevara
Functional differentiation of ...
Rhodococcus ruber, Rhodococcus ruber Chol-4
Microb. Cell Fact.
16
42
2017
-
-
-
-
-
-
-
20
-
-
-
-
-
7
-
-
-
-
-
-
-
-
33
1
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
20
-
-
-
-
-
-
-
-
-
-
-
-
33
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
743031
Shao
A mutant form of 3-ketosteroi ...
Mycolicibacterium neoaurum, Mycolicibacterium neoaurum JC-12, Mycolicibacterium neoaurum ST-095
J. Ind. Microbiol. Biotechnol.
43
691-701
2016
-
-
1
-
1
-
-
2
-
-
-
-
-
4
-
-
-
-
-
-
1
-
3
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
-
3
1
-
-
-
2
-
-
-
-
-
-
-
-
2
2
743221
Yao
Characterization and engineer ...
Mycolicibacterium neoaurum, Mycolicibacterium neoaurum ATCC 25795
Metab. Eng.
24
181-191
2014
-
1
1
-
-
-
-
14
-
-
-
-
-
6
-
-
-
-
-
-
-
-
28
1
3
-
-
-
-
-
-
-
-
-
-
-
-
3
3
-
-
-
-
-
-
-
14
-
-
-
-
-
-
-
-
-
-
-
-
28
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
724670
Brzezinska
The role of 3-ketosteroid 1(2) ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
BMC Microbiol.
13
43
2013
-
-
-
-
-
-
-
-
-
-
-
-
-
175
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
1
1
-
-
725830
Zhang
Bioconversion of 4-androstene- ...
Mycolicibacterium neoaurum, Mycolicibacterium neoaurum JC-12
J. Steroid Biochem. Mol. Biol.
135
36-42
2013
-
-
1
-
-
-
-
-
-
-
-
2
-
3
-
-
-
-
-
-
1
-
4
-
2
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
1
-
4
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
742850
Rohman
Crystal structure and site-di ...
Rhodococcus erythropolis, Rhodococcus erythropolis SQ1
J. Biol. Chem.
288
35559-35568
2013
-
-
1
1
3
-
-
3
-
-
-
-
-
4
-
-
-
-
-
-
1
-
6
-
2
-
-
-
3
-
-
-
1
-
-
-
-
-
1
1
1
3
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
1
-
6
-
-
-
-
3
-
-
-
-
-
-
-
-
3
3
723868
Rohman
Purification, crystallization ...
Rhodococcus erythropolis, Rhodococcus erythropolis SQ1
Acta Crystallogr. Sect. F
68
551-556
2012
-
-
1
1
-
-
-
-
-
-
1
2
-
4
-
-
1
-
-
-
-
-
2
1
2
-
-
-
-
-
-
1
1
-
-
-
-
-
1
1
1
-
-
-
-
-
-
-
-
1
2
-
-
-
1
-
-
-
-
2
1
-
-
-
-
-
-
1
-
-
1
1
-
-
-
724074
Chen
Characterization and applicati ...
Aspergillus fumigatus, Aspergillus fumigatus CICC 40167
Appl. Microbiol. Biotechnol.
96
133-142
2012
-
1
1
-
-
-
-
-
1
-
1
2
-
2
-
-
1
-
-
-
-
-
2
1
3
1
-
-
-
1
-
-
2
-
1
-
-
1
1
2
-
-
-
-
-
-
-
1
-
1
2
-
-
-
1
-
-
-
-
2
1
1
-
-
-
1
-
-
1
-
1
1
-
-
-
724000
Wei
Inactivation and augmentation ...
Mycolicibacterium neoaurum, Mycolicibacterium neoaurum NwIB-01
Appl. Environ. Microbiol.
76
4578-4582
2010
-
1
1
-
-
-
-
-
-
-
-
2
-
2
-
-
1
-
-
-
1
-
6
-
3
1
-
-
-
1
-
-
1
-
-
-
-
1
1
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
1
-
-
1
-
6
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
684994
Knol
3-Keto-5alpha-steroid DELTA(1) ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv, Rhodococcus erythropolis, Rhodococcus erythropolis SQ1
Biochem. J.
410
339-346
2008
-
-
1
-
-
1
-
23
-
-
3
-
-
184
-
-
1
-
-
-
-
-
49
-
13
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
1
-
-
-
23
-
-
3
-
-
-
-
1
-
-
-
-
49
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
688748
Li
Expression of ksdD gene encodi ...
Pimelobacter simplex
Lett. Appl. Microbiol.
44
563-568
2007
-
-
1
-
-
-
-
-
-
-
1
-
-
4
-
-
-
-
-
-
2
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
675829
Brzostek
Identification and targeted di ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Ra / ATCC 25177, Mycolicibacterium smegmatis, Mycolicibacterium smegmatis mc(2)155 / ATCC 700084
Microbiology
151
2393-2402
2005
-
2
2
-
1
-
-
-
-
-
-
-
-
31
-
-
-
-
-
-
-
-
2
-
5
-
-
-
-
-
-
-
1
-
-
-
-
2
2
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
654270
Horinouchi
A new bacterial steroid degrad ...
Comamonas testosteroni, Comamonas testosteroni TA441
Appl. Environ. Microbiol.
69
4421-4430
2003
-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
657152
Adham
-
Biochemical studies on the mic ...
Pseudomonas fluorescens
Proc. Biochem.
38
897-902
2003
1
-
-
-
-
-
5
-
-
6
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
5
-
-
-
6
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
656722
van der Geize
Molecular and functional chara ...
Rhodococcus erythropolis, Rhodococcus erythropolis SQ1
Microbiology
148
3285-3292
2002
-
-
1
-
2
-
-
2
-
-
-
4
-
6
-
-
-
-
-
-
-
-
8
-
2
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
2
-
-
-
4
-
-
-
-
-
-
-
-
8
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
391391
Van der Geize
Unmarked gene deletion mutagen ...
Rhodococcus erythropolis
FEMS Microbiol. Lett.
205
197-202
2001
-
-
1
-
-
-
-
-
-
-
-
-
-
6
-
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1
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-
391394
Fujii
Essential tyrosine residues in ...
Rhodococcus rhodochrous
J. Biochem.
126
662-667
1999
-
-
1
-
-
-
1
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1
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1
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-
-
391396
Morii
3-Ketosteroid-DELTA1-dehydroge ...
Rhodococcus rhodochrous
J. Biochem.
124
1026-1032
1998
-
-
1
-
-
-
-
1
1
-
1
-
-
5
-
-
1
-
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1
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1
1
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1
1
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1
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1
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391392
Choi
Purification and characterizat ...
Pimelobacter simplex
J. Biochem.
117
1043-1049
1995
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-
-
-
-
-
5
1
-
-
1
-
1
1
-
-
1
-
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1
5
1
-
1
1
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1
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5
-
1
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1
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1
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1
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1
5
1
1
1
-
-
1
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-
-
-
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-
-
-
-
391393
Choi
Secretory overproduction of Ar ...
Pimelobacter simplex
Appl. Microbiol. Biotechnol.
43
1044-1049
1995
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-
-
-
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1
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1
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1
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-
391395
Molnar
Molecular cloning, expression ...
Pimelobacter simplex
Mol. Microbiol.
15
895-905
1995
-
-
1
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1
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1
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1
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1
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391375
Itagaki
Purification and characterizat ...
Gordonia rubripertincta
Biochim. Biophys. Acta
1038
60-67
1990
-
-
-
-
-
-
2
11
-
-
1
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-
1
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-
1
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-
1
1
15
1
-
1
-
2
-
2
2
-
1
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-
1
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-
-
2
-
11
-
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1
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1
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-
1
1
15
1
1
-
2
-
2
2
-
-
-
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-
-
-
-
391378
Itagaki
Spectral properties of 3-ketos ...
Gordonia rubripertincta
Biochim. Biophys. Acta
1040
281-286
1990
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-
-
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1
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1
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2
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1
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1
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1
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2
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391379
Kloosterman IV
-
Effect of supersaturated aqueo ...
Pimelobacter simplex
Enzyme Microb. Technol.
6
113-116
1984
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-
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1
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1
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1
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-
391385
Udvardy
-
Kinetics of the formation of s ...
Pimelobacter simplex
FEMS Symp.
13
21-33
1982
-
-
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1
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-
391384
Atrat
Steroid transformation with im ...
Mycolicibacterium phlei, Nocardia erythropolis, Rhodococcus opacus
Z. Allg. Mikrobiol.
21
3-6
1981
-
-
-
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-
3
-
-
-
-
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-
3
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3
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-
391376
Atrat
-
Efficient purification of a mi ...
Rhodococcus opacus
J. Chromatogr.
189
279-283
1980
-
-
-
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1
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-
1
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1
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-
-
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-
391389
Hrhold
Steroid transforming enzymes i ...
Rhodococcus opacus
Z. Allg. Mikrobiol.
20
23-32
1980
-
-
-
-
-
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-
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1
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-
-
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-
391377
Groh
Steroid-transforming enzymes f ...
Rhodococcus opacus
Z. Allg. Mikrobiol.
19
727-730
1979
-
-
-
-
-
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-
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3
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2
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-
2
-
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-
-
-
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-
-
-
-
391388
Hrhold
Steroid-transforming enzymes f ...
Rhodococcus opacus
Z. Allg. Mikrobiol.
19
731-739
1979
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-
-
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1
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-
391387
Lestrovaja
Steroid transforming enzymes f ...
Rhodococcus opacus
Z. Allg. Mikrobiol.
18
189-196
1978
-
-
-
-
-
-
1
1
-
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-
1
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-
1
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1
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2
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1
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1
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1
-
1
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1
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1
-
2
-
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-
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-
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-
391390
Abul-Hajj
Isolation of vitamin K2(35) fr ...
Ilyonectria destructans, Pimelobacter simplex, Plectosphaerella cucumerina, Rhodococcus hoagii
J. Biol. Chem.
253
2356-2360
1978
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4
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15
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15
-
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-
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-
391386
Hrhold
Steroid-transforming enzymes f ...
Rhodococcus opacus
Z. Allg. Mikrobiol.
16
559-561
1976
-
-
-
-
-
-
-
-
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1
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-
1
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1
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-
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-
-
-
-
-
-
-
-
-
391380
Penasse
The active center of the 3-oxo ...
Pimelobacter simplex
Eur. J. Biochem.
47
555-559
1974
-
-
-
-
-
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-
1
-
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1
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1
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1
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-
1
-
-
-
-
-
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-
-
-
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-
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-
-
391382
Rodig
The stereochemistry of hydroge ...
Saccharomyces cerevisiae
Biochem. Biophys. Res. Commun.
56
467-470
1974
-
-
-
-
-
-
-
-
-
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-
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-
2
-
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-
1
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1
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-
1
-
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-
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-
-
-
-
-
-
-
-
391381
Aries
-
Degradation of steroids by int ...
Clostridium paraputrificum
Biochim. Biophys. Acta
248
482-488
1971
-
-
-
-
-
-
-
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-
1
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5
-
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1
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-
5
-
-
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-
1
-
-
-
-
-
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-
-
391383
Levy
Bacterial oxidation of steroid ...
Comamonas testosteroni
J. Biol. Chem.
234
2014-2021
1959
-
-
-
-
-
-
8
-
1
-
-
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-
1
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-
1
-
-
-
-
-
9
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
8
-
-
1
-
-
-
-
-
-
1
-
-
-
-
9
-
1
-
-
-
1
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-