Literature summary for 1.3.99.4 extracted from

Brzostek, A.; Sliwinski, T.; Rumijowska-Galewicz, A.; Korycka-Machala, M.; Dziadek, J.
Identification and targeted disruption of the gene encoding the main 3-ketosteroid dehydrogenase in Mycobacterium smegmatis (2005), Microbiology, 151, 2393-2402.

Search for more literature for 1.3.99.4

Application

Application	Comment	Organism
additional information	ksdD-1 and ksdD-2 display respectively high (78%) and low (33%) amino acid sequence identity with the putative ksdD gene of Mycobacterium tuberculosis	Mycolicibacterium smegmatis
additional information	the two putative Mycobacterium tuberculosis KsdDs, MT3641 and MT0809, complement the Mycobacterium smegmatis deltaksdD-1 deltaksdD-2 double mutant	Mycobacterium tuberculosis

Cloned(Commentary)

Cloned (Comment)	Organism
into the pJam2 expression vector under the acetamidase promoter and introduced into the deltaksdD-1 mutant	Mycolicibacterium smegmatis
under control of the Phsp promoter and introduced into mutant cells of Mycobacterium smegmatis	Mycobacterium tuberculosis

Protein Variants

Protein Variants	Comment	Organism
additional information	targeted disruption of the MSMEG5898 (ksdD-1) gene, but not the MSMEG4855 (ksdD-2) gene, results in partial inactivation of the cholesterol degradation pathway and accumulation of the intermediate 4-androstene-3,17-dione, reversible by the introduction of the wild-type ksdD-1 gene into deltaksdD-1 or overexpression of ksdD-2	Mycolicibacterium smegmatis

Organism

Organism	UniProt	Comment	Textmining
Mycobacterium tuberculosis	-	-	-
Mycobacterium tuberculosis H37Ra / ATCC 25177	-	-	-
Mycolicibacterium smegmatis	-	-	-
Mycolicibacterium smegmatis mc(2)155 / ATCC 700084	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
cholesterol + acceptor	KsdD2 exhibits low but detectable activity in the cholesterol degradation process	Mycolicibacterium smegmatis	?	-	?
cholesterol + acceptor	KsdD2 exhibits low but detectable activity in the cholesterol degradation process	Mycolicibacterium smegmatis mc(2)155 / ATCC 700084	?	-	?

Synonyms

Synonyms	Comment	Organism
3-ketosteroid delta1-dehydrogenase	-	Mycolicibacterium smegmatis
3-ketosteroid delta1-dehydrogenase	-	Mycobacterium tuberculosis
KsdD	-	Mycobacterium tuberculosis
KsdD1	-	Mycolicibacterium smegmatis
KsdD2	-	Mycolicibacterium smegmatis

Cofactor

Cofactor	Comment	Organism	Structure
FAD	-	Mycolicibacterium smegmatis

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Release 2023.1 (January 2023)