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Literature summary for 1.3.99.32 extracted from

  • Wischgoll, S.; Taubert, M.; Peters, F.; Jehmlich, N.; von Bergen, M.; Boll, M.
    Decarboxylating and nondecarboxylating glutaryl-coenzyme A dehydrogenases in the aromatic metabolism of obligately anaerobic bacteria (2009), J. Bacteriol., 191, 4401-4409.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.3.99.32

Cloned(Commentary)

Cloned (Comment) Organism
screening of a cosmid gene library, DNA and amino acid sequence determination and analysis, expression of His6-tagged GDHDes in Escherichia coli strain BL21(DE3) Desulfococcus multivorans

Inhibitors

Inhibitors Comment Organism Structure
glutaconyl-CoA product inhibition Desulfococcus multivorans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.052
-
 glutaryl-CoA pH 7.8, 30°C Desulfococcus multivorans

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrial inner membrane
-
 Desulfococcus multivorans 5743
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glutaryl-CoA + FAD Desulfococcus multivorans
-
 glutaconyl-CoA + FADH2
-
 ?
additional information Desulfococcus multivorans GDHGeo contains highly conserved amino acid residues that are specifically involved in the decarboxylation of the intermediate glutaconyl-CoA ?
-
 ?

Organism

Organism UniProt Comment Textmining
Desulfococcus multivorans C3UVB0 a sulfate reducing organism
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged GDHDes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Desulfococcus multivorans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutaryl-CoA + FAD
-
 Desulfococcus multivorans glutaconyl-CoA + FADH2
-
 ?
additional information GDHGeo contains highly conserved amino acid residues that are specifically involved in the decarboxylation of the intermediate glutaconyl-CoA Desulfococcus multivorans ?
-
 ?

Subunits

Subunits Comment Organism
homotetramer 4 * 43000-44000 Desulfococcus multivorans

Synonyms

Synonyms Comment Organism
GDH
-
 Desulfococcus multivorans
GDHDes
-
 Desulfococcus multivorans
nondecarboxylating glutaryl-coenzyme A dehydrogenase
-
 Desulfococcus multivorans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Desulfococcus multivorans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
 assay at Desulfococcus multivorans

Cofactor

Cofactor Comment Organism Structure
FAD GDHDes contains 0.6-0.7 FAD/monomer Desulfococcus multivorans