Cloned (Comment) | Organism |
---|---|
gene carB, sequence comparisons and phylogenetic analysis, recombinant expression in Escherichia coli | Blakeslea trispora |
gene crtI, sequence comparisons and phylogenetic analysis, recombinant expression in Escherichia coli | Pantoea ananatis |
gene crtI, sequence comparisons and phylogenetic analysis, recombinant expression in Escherichia coli | Mycolicibacterium aurum |
gene crtI, sequence comparisons and phylogenetic analysis, recombinant expression in Escherichia coli | Pantoea agglomerans |
gene Rhu_A0493, sequence comparisons and phylogenetic analysis, recombinant expression in Escherichia coli | Rhodospirillum rubrum |
Protein Variants | Comment | Organism |
---|---|---|
additional information | competition between lycopene cyclase and the phytoene desaturases modified the distribution between carotene intermediates when expressed in yeast in the context of the full beta-carotene production pathway | Pantoea ananatis |
additional information | competition between lycopene cyclase and the phytoene desaturases modified the distribution between carotene intermediates when expressed in yeast in the context of the full beta-carotene production pathway | Blakeslea trispora |
additional information | competition between lycopene cyclase and the phytoene desaturases modified the distribution between carotene intermediates when expressed in yeast in the context of the full beta-carotene production pathway | Mycolicibacterium aurum |
additional information | competition between lycopene cyclase and the phytoene desaturases modified the distribution between carotene intermediates when expressed in yeast in the context of the full beta-carotene production pathway | Rhodospirillum rubrum |
additional information | competition between lycopene cyclase and the phytoene desaturases modified the distribution between carotene intermediates when expressed in yeast in the context of the full beta-carotene production pathway | Pantoea agglomerans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | classical Michaelis-Menten kinetic model | Pantoea ananatis | |
additional information | - |
additional information | classical Michaelis-Menten kinetic model | Blakeslea trispora | |
additional information | - |
additional information | classical Michaelis-Menten kinetic model | Mycolicibacterium aurum | |
additional information | - |
additional information | classical Michaelis-Menten kinetic model | Rhodospirillum rubrum | |
additional information | - |
additional information | classical Michaelis-Menten kinetic model | Pantoea agglomerans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Blakeslea trispora | 16020 | - |
membrane | - |
Mycolicibacterium aurum | 16020 | - |
membrane | - |
Rhodospirillum rubrum | 16020 | - |
membrane | - |
Pantoea agglomerans | 16020 | - |
membrane | CrtI from Pantoea ananas associates spontaneously to liposomal membranes but no membrane-spanning region per se is evidenced, suggesting a monotopic binding to membranes | Pantoea ananatis | 16020 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
15-cis-phytoene + acceptor | Pantoea ananatis | - |
all-trans-phytofluene + reduced acceptor | - |
? | |
15-cis-phytoene + acceptor | Blakeslea trispora | - |
all-trans-phytofluene + reduced acceptor | - |
? | |
15-cis-phytoene + acceptor | Mycolicibacterium aurum | - |
all-trans-phytofluene + reduced acceptor | - |
? | |
15-cis-phytoene + acceptor | Rhodospirillum rubrum | - |
all-trans-phytofluene + reduced acceptor | - |
? | |
15-cis-phytoene + acceptor | Pantoea agglomerans | - |
all-trans-phytofluene + reduced acceptor | - |
? | |
15-cis-phytoene + acceptor | Rhodospirillum rubrum S1 | - |
all-trans-phytofluene + reduced acceptor | - |
? | |
15-cis-phytoene + acceptor | Rhodospirillum rubrum NCIMB 8255 | - |
all-trans-phytofluene + reduced acceptor | - |
? | |
15-cis-phytoene + acceptor | Rhodospirillum rubrum ATH 1.1.1 | - |
all-trans-phytofluene + reduced acceptor | - |
? | |
15-cis-phytoene + acceptor | Rhodospirillum rubrum ATCC 11170 | - |
all-trans-phytofluene + reduced acceptor | - |
? | |
15-cis-phytoene + acceptor | Rhodospirillum rubrum LMG 4362 | - |
all-trans-phytofluene + reduced acceptor | - |
? | |
15-cis-phytoene + acceptor | Rhodospirillum rubrum DSM 467 | - |
all-trans-phytofluene + reduced acceptor | - |
? | |
all-trans-neurosporene + acceptor | Pantoea ananatis | - |
all-trans-lycopene + reduced acceptor | - |
? | |
all-trans-neurosporene + acceptor | Blakeslea trispora | - |
all-trans-lycopene + reduced acceptor | - |
? | |
all-trans-neurosporene + acceptor | Mycolicibacterium aurum | - |
all-trans-lycopene + reduced acceptor | - |
? | |
all-trans-neurosporene + acceptor | Rhodospirillum rubrum | - |
all-trans-lycopene + reduced acceptor | - |
? | |
all-trans-neurosporene + acceptor | Pantoea agglomerans | - |
all-trans-lycopene + reduced acceptor | - |
? | |
all-trans-neurosporene + acceptor | Rhodospirillum rubrum S1 | - |
all-trans-lycopene + reduced acceptor | - |
? | |
all-trans-neurosporene + acceptor | Rhodospirillum rubrum NCIMB 8255 | - |
all-trans-lycopene + reduced acceptor | - |
? | |
all-trans-neurosporene + acceptor | Rhodospirillum rubrum ATH 1.1.1 | - |
all-trans-lycopene + reduced acceptor | - |
? | |
all-trans-neurosporene + acceptor | Rhodospirillum rubrum ATCC 11170 | - |
all-trans-lycopene + reduced acceptor | - |
? | |
all-trans-neurosporene + acceptor | Rhodospirillum rubrum LMG 4362 | - |
all-trans-lycopene + reduced acceptor | - |
? | |
all-trans-neurosporene + acceptor | Rhodospirillum rubrum DSM 467 | - |
all-trans-lycopene + reduced acceptor | - |
? | |
all-trans-phytofluene + acceptor | Pantoea ananatis | - |
all-trans-zeta-carotene + reduced acceptor | - |
? | |
all-trans-phytofluene + acceptor | Blakeslea trispora | - |
all-trans-zeta-carotene + reduced acceptor | - |
? | |
all-trans-phytofluene + acceptor | Mycolicibacterium aurum | - |
all-trans-zeta-carotene + reduced acceptor | - |
? | |
all-trans-phytofluene + acceptor | Rhodospirillum rubrum | - |
all-trans-zeta-carotene + reduced acceptor | - |
? | |
all-trans-phytofluene + acceptor | Pantoea agglomerans | - |
all-trans-zeta-carotene + reduced acceptor | - |
? | |
all-trans-phytofluene + acceptor | Rhodospirillum rubrum S1 | - |
all-trans-zeta-carotene + reduced acceptor | - |
? | |
all-trans-phytofluene + acceptor | Rhodospirillum rubrum NCIMB 8255 | - |
all-trans-zeta-carotene + reduced acceptor | - |
? | |
all-trans-phytofluene + acceptor | Rhodospirillum rubrum ATH 1.1.1 | - |
all-trans-zeta-carotene + reduced acceptor | - |
? | |
all-trans-phytofluene + acceptor | Rhodospirillum rubrum ATCC 11170 | - |
all-trans-zeta-carotene + reduced acceptor | - |
? | |
all-trans-phytofluene + acceptor | Rhodospirillum rubrum LMG 4362 | - |
all-trans-zeta-carotene + reduced acceptor | - |
? | |
all-trans-phytofluene + acceptor | Rhodospirillum rubrum DSM 467 | - |
all-trans-zeta-carotene + reduced acceptor | - |
? | |
all-trans-zeta-carotene + acceptor | Pantoea ananatis | - |
all-trans-neurosporene + reduced acceptor | - |
? | |
all-trans-zeta-carotene + acceptor | Blakeslea trispora | - |
all-trans-neurosporene + reduced acceptor | - |
? | |
all-trans-zeta-carotene + acceptor | Mycolicibacterium aurum | - |
all-trans-neurosporene + reduced acceptor | - |
? | |
all-trans-zeta-carotene + acceptor | Rhodospirillum rubrum | - |
all-trans-neurosporene + reduced acceptor | - |
? | |
all-trans-zeta-carotene + acceptor | Pantoea agglomerans | - |
all-trans-neurosporene + reduced acceptor | - |
? | |
all-trans-zeta-carotene + acceptor | Rhodospirillum rubrum S1 | - |
all-trans-neurosporene + reduced acceptor | - |
? | |
all-trans-zeta-carotene + acceptor | Rhodospirillum rubrum NCIMB 8255 | - |
all-trans-neurosporene + reduced acceptor | - |
? | |
all-trans-zeta-carotene + acceptor | Rhodospirillum rubrum ATH 1.1.1 | - |
all-trans-neurosporene + reduced acceptor | - |
? | |
all-trans-zeta-carotene + acceptor | Rhodospirillum rubrum ATCC 11170 | - |
all-trans-neurosporene + reduced acceptor | - |
? | |
all-trans-zeta-carotene + acceptor | Rhodospirillum rubrum LMG 4362 | - |
all-trans-neurosporene + reduced acceptor | - |
? | |
all-trans-zeta-carotene + acceptor | Rhodospirillum rubrum DSM 467 | - |
all-trans-neurosporene + reduced acceptor | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Blakeslea trispora | Q67GI0 | Choanephora trispora | - |
Mycolicibacterium aurum | Q9K566 | Mycobacterium aurum | - |
Pantoea agglomerans | E9LFG2 | Erwinia herbicola or Pantoea agglomerans | - |
Pantoea ananatis | P21685 | Erwinia uredovora | - |
Rhodospirillum rubrum | Q2RX47 | - |
- |
Rhodospirillum rubrum ATCC 11170 | Q2RX47 | - |
- |
Rhodospirillum rubrum ATH 1.1.1 | Q2RX47 | - |
- |
Rhodospirillum rubrum DSM 467 | Q2RX47 | - |
- |
Rhodospirillum rubrum LMG 4362 | Q2RX47 | - |
- |
Rhodospirillum rubrum NCIMB 8255 | Q2RX47 | - |
- |
Rhodospirillum rubrum S1 | Q2RX47 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
15-cis-phytoene + acceptor | - |
Pantoea ananatis | all-trans-phytofluene + reduced acceptor | - |
? | |
15-cis-phytoene + acceptor | - |
Blakeslea trispora | all-trans-phytofluene + reduced acceptor | - |
? | |
15-cis-phytoene + acceptor | - |
Mycolicibacterium aurum | all-trans-phytofluene + reduced acceptor | - |
? | |
15-cis-phytoene + acceptor | - |
Rhodospirillum rubrum | all-trans-phytofluene + reduced acceptor | - |
? | |
15-cis-phytoene + acceptor | - |
Pantoea agglomerans | all-trans-phytofluene + reduced acceptor | - |
? | |
15-cis-phytoene + acceptor | - |
Rhodospirillum rubrum S1 | all-trans-phytofluene + reduced acceptor | - |
? | |
15-cis-phytoene + acceptor | - |
Rhodospirillum rubrum NCIMB 8255 | all-trans-phytofluene + reduced acceptor | - |
? | |
15-cis-phytoene + acceptor | - |
Rhodospirillum rubrum ATH 1.1.1 | all-trans-phytofluene + reduced acceptor | - |
? | |
15-cis-phytoene + acceptor | - |
Rhodospirillum rubrum ATCC 11170 | all-trans-phytofluene + reduced acceptor | - |
? | |
15-cis-phytoene + acceptor | - |
Rhodospirillum rubrum LMG 4362 | all-trans-phytofluene + reduced acceptor | - |
? | |
15-cis-phytoene + acceptor | - |
Rhodospirillum rubrum DSM 467 | all-trans-phytofluene + reduced acceptor | - |
? | |
all-trans-neurosporene + acceptor | - |
Pantoea ananatis | all-trans-lycopene + reduced acceptor | - |
? | |
all-trans-neurosporene + acceptor | - |
Blakeslea trispora | all-trans-lycopene + reduced acceptor | - |
? | |
all-trans-neurosporene + acceptor | - |
Mycolicibacterium aurum | all-trans-lycopene + reduced acceptor | - |
? | |
all-trans-neurosporene + acceptor | - |
Rhodospirillum rubrum | all-trans-lycopene + reduced acceptor | - |
? | |
all-trans-neurosporene + acceptor | - |
Pantoea agglomerans | all-trans-lycopene + reduced acceptor | - |
? | |
all-trans-neurosporene + acceptor | - |
Rhodospirillum rubrum S1 | all-trans-lycopene + reduced acceptor | - |
? | |
all-trans-neurosporene + acceptor | - |
Rhodospirillum rubrum NCIMB 8255 | all-trans-lycopene + reduced acceptor | - |
? | |
all-trans-neurosporene + acceptor | - |
Rhodospirillum rubrum ATH 1.1.1 | all-trans-lycopene + reduced acceptor | - |
? | |
all-trans-neurosporene + acceptor | - |
Rhodospirillum rubrum ATCC 11170 | all-trans-lycopene + reduced acceptor | - |
? | |
all-trans-neurosporene + acceptor | - |
Rhodospirillum rubrum LMG 4362 | all-trans-lycopene + reduced acceptor | - |
? | |
all-trans-neurosporene + acceptor | - |
Rhodospirillum rubrum DSM 467 | all-trans-lycopene + reduced acceptor | - |
? | |
all-trans-phytofluene + acceptor | - |
Pantoea ananatis | all-trans-zeta-carotene + reduced acceptor | - |
? | |
all-trans-phytofluene + acceptor | - |
Blakeslea trispora | all-trans-zeta-carotene + reduced acceptor | - |
? | |
all-trans-phytofluene + acceptor | - |
Mycolicibacterium aurum | all-trans-zeta-carotene + reduced acceptor | - |
? | |
all-trans-phytofluene + acceptor | - |
Rhodospirillum rubrum | all-trans-zeta-carotene + reduced acceptor | - |
? | |
all-trans-phytofluene + acceptor | - |
Pantoea agglomerans | all-trans-zeta-carotene + reduced acceptor | - |
? | |
all-trans-phytofluene + acceptor | - |
Rhodospirillum rubrum S1 | all-trans-zeta-carotene + reduced acceptor | - |
? | |
all-trans-phytofluene + acceptor | - |
Rhodospirillum rubrum NCIMB 8255 | all-trans-zeta-carotene + reduced acceptor | - |
? | |
all-trans-phytofluene + acceptor | - |
Rhodospirillum rubrum ATH 1.1.1 | all-trans-zeta-carotene + reduced acceptor | - |
? | |
all-trans-phytofluene + acceptor | - |
Rhodospirillum rubrum ATCC 11170 | all-trans-zeta-carotene + reduced acceptor | - |
? | |
all-trans-phytofluene + acceptor | - |
Rhodospirillum rubrum LMG 4362 | all-trans-zeta-carotene + reduced acceptor | - |
? | |
all-trans-phytofluene + acceptor | - |
Rhodospirillum rubrum DSM 467 | all-trans-zeta-carotene + reduced acceptor | - |
? | |
all-trans-zeta-carotene + acceptor | - |
Pantoea ananatis | all-trans-neurosporene + reduced acceptor | - |
? | |
all-trans-zeta-carotene + acceptor | - |
Blakeslea trispora | all-trans-neurosporene + reduced acceptor | - |
? | |
all-trans-zeta-carotene + acceptor | - |
Mycolicibacterium aurum | all-trans-neurosporene + reduced acceptor | - |
? | |
all-trans-zeta-carotene + acceptor | - |
Rhodospirillum rubrum | all-trans-neurosporene + reduced acceptor | - |
? | |
all-trans-zeta-carotene + acceptor | - |
Pantoea agglomerans | all-trans-neurosporene + reduced acceptor | - |
? | |
all-trans-zeta-carotene + acceptor | - |
Rhodospirillum rubrum S1 | all-trans-neurosporene + reduced acceptor | - |
? | |
all-trans-zeta-carotene + acceptor | - |
Rhodospirillum rubrum NCIMB 8255 | all-trans-neurosporene + reduced acceptor | - |
? | |
all-trans-zeta-carotene + acceptor | - |
Rhodospirillum rubrum ATH 1.1.1 | all-trans-neurosporene + reduced acceptor | - |
? | |
all-trans-zeta-carotene + acceptor | - |
Rhodospirillum rubrum ATCC 11170 | all-trans-neurosporene + reduced acceptor | - |
? | |
all-trans-zeta-carotene + acceptor | - |
Rhodospirillum rubrum LMG 4362 | all-trans-neurosporene + reduced acceptor | - |
? | |
all-trans-zeta-carotene + acceptor | - |
Rhodospirillum rubrum DSM 467 | all-trans-neurosporene + reduced acceptor | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CarB | - |
Blakeslea trispora |
CrtI | - |
Pantoea ananatis |
CrtI | - |
Blakeslea trispora |
CrtI | - |
Mycolicibacterium aurum |
CrtI | - |
Rhodospirillum rubrum |
CrtI | - |
Pantoea agglomerans |
PDS | - |
Pantoea ananatis |
PDS | - |
Blakeslea trispora |
PDS | - |
Mycolicibacterium aurum |
PDS | - |
Rhodospirillum rubrum |
PDS | - |
Pantoea agglomerans |
phytoene desaturase | - |
Pantoea ananatis |
phytoene desaturase | - |
Blakeslea trispora |
phytoene desaturase | - |
Mycolicibacterium aurum |
phytoene desaturase | - |
Rhodospirillum rubrum |
phytoene desaturase | - |
Pantoea agglomerans |
Rru_A0493 | - |
Rhodospirillum rubrum |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the CrtI family of enzymes, analysis of the phylogenetic tree of a subset of phytoene desaturases from the CrtI family, overview. Recombinant expression of eight codon optimized CrtI enzymes from different clades in a bacterial system reveals that three CrtI enzymes can catalyse up to six desaturations, forming tetradehydrolycopene. Existence of characteristic patterns of desaturated molecules associated with various CrtI clades. Variations in the reaction rates and binding constants can explain the various carotene patterns observed. Relationship between genetic and functional evolution of certain CrtI enzymes, overview | Pantoea ananatis |
evolution | the enzyme belongs to the CrtI family of enzymes, analysis of the phylogenetic tree of a subset of phytoene desaturases from the CrtI family, overview. Recombinant expression of eight codon optimized CrtI enzymes from different clades in a bacterial system reveals that three CrtI enzymes can catalyse up to six desaturations, forming tetradehydrolycopene. Existence of characteristic patterns of desaturated molecules associated with various CrtI clades. Variations in the reaction rates and binding constants can explain the various carotene patterns observed. Relationship between genetic and functional evolution of certain CrtI enzymes, overview | Blakeslea trispora |
evolution | the enzyme belongs to the CrtI family of enzymes, analysis of the phylogenetic tree of a subset of phytoene desaturases from the CrtI family, overview. Recombinant expression of eight codon optimized CrtI enzymes from different clades in a bacterial system reveals that three CrtI enzymes can catalyse up to six desaturations, forming tetradehydrolycopene. Existence of characteristic patterns of desaturated molecules associated with various CrtI clades. Variations in the reaction rates and binding constants can explain the various carotene patterns observed. Relationship between genetic and functional evolution of certain CrtI enzymes, overview | Mycolicibacterium aurum |
evolution | the enzyme belongs to the CrtI family of enzymes, analysis of the phylogenetic tree of a subset of phytoene desaturases from the CrtI family, overview. Recombinant expression of eight codon optimized CrtI enzymes from different clades in a bacterial system reveals that three CrtI enzymes can catalyse up to six desaturations, forming tetradehydrolycopene. Existence of characteristic patterns of desaturated molecules associated with various CrtI clades. Variations in the reaction rates and binding constants can explain the various carotene patterns observed. Relationship between genetic and functional evolution of certain CrtI enzymes, overview | Rhodospirillum rubrum |
evolution | the enzyme belongs to the CrtI family of enzymes, analysis of the phylogenetic tree of a subset of phytoene desaturases from the CrtI family, overview. Recombinant expression of eight codon optimized CrtI enzymes from different clades in a bacterial system reveals that three CrtI enzymes can catalyse up to six desaturations, formingtetradehydrolycopene. Existence of characteristic patterns of desaturated molecules associated with various CrtI clades. Variations in the reaction rates and binding constants can explain the various carotene patterns observed. Relationship between genetic and functional evolution of certain CrtI enzymes, overview | Pantoea agglomerans |
metabolism | carotenoid biosynthesis starts with the symmetrical condensation of two geranylgeranyl diphosphate molecules, forming phytoene. A series of successive desaturation reactions convert phytoene into phytofluene, zeta-carotene, neurosporene, lycopene. These desaturation reactions can be accomplished by a single enzyme (poly-trans pathway) or through a cascade of different enzymes (poly-cis pathway). In algae and plants, four different enzymes are necessary to form the final product (all-trans-lycopene). The phytoene and the zeta-carotene desaturases (PDS and ZDS, respectively) add double bonds in the cis-conformation. ZISO (zeta-carotene isomerase) and CRTISO (prolycopene isomerase) convert the cis-carotenes into di-cis-zeta-carotene and all-trans-lycopene, respectively. By contrast to other phytoene desaturases, CrtI are versatile enzymes classified into four enzymatic subgroups (EC 1.3.99.28, EC 1.3.99.29, EC 1.3.99.30, and EC 1.3.99.31) based on the last product they presumably produce (from zeta-carotene to didehydrolycopene). Carotene diversity can be further expanded in later steps with the addition of one or two rings by lycopene cyclases, thereby producing an extensive variety of symmetrical or asymmetrical cyclised carotenes, such as beta-zeacarotene, dehydro-beta-carotene, gamma-carotene, beta-carotene, and the fungi-specific torulene. When expressed in heterologous hosts, CrtI enzymes exhibit distinct desaturation patterns, CrtI enzyme activities may depend on the experimental conditions and thus be inconsistent with the patterns generated in the natural host. Pantoea ananatis CrtI produces lycopene in vivo, but also tetradehydrolycopene in vitro | Pantoea ananatis |
metabolism | carotenoid biosynthesis starts with the symmetrical condensation of two geranylgeranyl diphosphate molecules, forming phytoene. A series of successive desaturation reactions convert phytoene into phytofluene, zeta-carotene, neurosporene, lycopene. These desaturation reactions can be accomplished by a single enzyme (poly-trans pathway) or through a cascade of different enzymes (poly-cis pathway). In algae and plants, four different enzymes are necessary to form the final product (all-trans-lycopene). The phytoene and the zeta-carotene desaturases (PDS and ZDS, respectively) add double bonds in the cis-conformation. ZISO (zeta-carotene isomerase) and CRTISO (prolycopene isomerase) convert the cis-carotenes into di-cis-zeta-carotene and all-trans-lycopene, respectively. By contrast to other phytoene desaturases, CrtI are versatile enzymes classified into four enzymatic subgroups (EC 1.3.99.28, EC 1.3.99.29, EC 1.3.99.30, and EC 1.3.99.31) based on the last product they presumably produce (from zeta-carotene to didehydrolycopene). Carotene diversity can be further expanded in later steps with the addition of one or two rings by lycopene cyclases, thereby producing an extensive variety of symmetrical or asymmetrical cyclised carotenes, such as beta-zeacarotene, dehydro-beta-carotene, gamma-carotene, beta-carotene, and the fungi-specific torulene. When expressed in heterologous hosts, CrtI enzymes exhibit distinct desaturation patterns, CrtI enzyme activities may depend on the experimental conditions and thus be inconsistent with the patterns generated in the natural host. Mycolicibacterium aurum CrtI produces lycopene in vivo and in vitro | Mycolicibacterium aurum |
metabolism | carotenoid biosynthesis starts with the symmetrical condensation of two geranylgeranyl diphosphate molecules, forming phytoene. A series of successive desaturation reactions convert phytoene into phytofluene, zeta-carotene, neurosporene, lycopene. These desaturation reactions can be accomplished by a single enzyme (poly-trans pathway) or through a cascade of different enzymes (poly-cis pathway). In algae and plants, four different enzymes are necessary to form the final product (all-trans-lycopene). The phytoene and the zeta-carotene desaturases (PDS and ZDS, respectively) add double bonds in the cis-conformation. ZISO (zeta-carotene isomerase) and CRTISO (prolycopene isomerase) convert the cis-carotenes into di-cis-zeta-carotene and all-trans-lycopene, respectively. By contrast to other phytoene desaturases, CrtI are versatile enzymes classified into four enzymatic subgroups (EC 1.3.99.28, EC 1.3.99.29, EC 1.3.99.30, and EC 1.3.99.31) based on the last product they presumably produce (from zeta-carotene to didehydrolycopene). Carotene diversity can be further expanded in later steps with the addition of one or two rings by lycopene cyclases, thereby producing an extensive variety of symmetrical or asymmetrical cyclised carotenes, such as beta-zeacarotene, dehydro-beta-carotene, gamma-carotene, beta-carotene, and the fungi-specific torulene. When expressed in heterologous hosts, CrtI enzymes exhibit distinct desaturation patterns, CrtI enzyme activities may depend on the experimental conditions and thus be inconsistent with the patterns generated in the natural host. Rhodospirillum rubrum CrtI produces lycopene in vivo and in vitro | Rhodospirillum rubrum |
metabolism | carotenoid biosynthesis starts with the symmetrical condensation of two geranylgeranyl diphosphate molecules, forming phytoene. A series of successive desaturation reactions convert phytoene into phytofluene, zeta-carotene, neurosporene, lycopene. These desaturation reactions can be accomplished by a single enzyme (poly-trans pathway) or through a cascade of different enzymes (poly-cis pathway). In algae and plants, four different enzymes are necessary to form the final product (all-trans-lycopene). The phytoene and the zeta-carotene desaturases (PDS and ZDS, respectively) add double bonds in the cis-conformation. ZISO (zeta-carotene isomerase) and CRTISO (prolycopene isomerase) convert the cis-carotenes into di-cis-zeta-carotene and all-trans-lycopene, respectively. By contrast to other phytoene desaturases, CrtI are versatile enzymes classified into four enzymatic subgroups (EC 1.3.99.28, EC 1.3.99.29, EC 1.3.99.30, and EC 1.3.99.31) based on the last product they presumably produce (from zeta-carotene to didehydrolycopene). Carotene diversity can be further expanded in later steps with the addition of one or two rings by lycopene cyclases, thereby producing an extensive variety of symmetrical or asymmetrical cyclised carotenes, such as beta-zeacarotene, dehydro-beta-carotene, gamma-carotene, beta-carotene, and the fungi-specific torulene. When expressed in heterologous hosts, CrtI enzymes exhibit distinct desaturation patterns, CrtI enzyme activities may depend on the experimental conditions and thus be inconsistent with the patterns generated in the natural host. Blakeslea trispora CrtI produces lycopene in vivo and in vitro, but also didehydrolycopene in vivo (see also EC 1.3.99.30) | Blakeslea trispora |
metabolism | carotenoid biosynthesis starts with the symmetrical condensation of two geranylgeranyl diphosphate molecules, forming phytoene. A series of successive desaturation reactions convert phytoene into phytofluene, zeta-carotene, neurosporene, lycopene. These desaturation reactions can be accomplished by a single enzyme (poly-trans pathway) or through a cascade of different enzymes (poly-cis pathway). In algae and plants, four different enzymes are necessary to form the final product (all-trans-lycopene). The phytoene and the zeta-carotene desaturases (PDS and ZDS, respectively) add double bonds in the cis-conformation. ZISO (zeta-carotene isomerase) and CRTISO (prolycopene isomerase) convert the cis-carotenes into di-cis-zeta-carotene and all-trans-lycopene, respectively. By contrast to other phytoene desaturases, CrtI are versatile enzymes classified into four enzymatic subgroups (EC 1.3.99.28, EC 1.3.99.29, EC 1.3.99.30, and EC 1.3.99.31) based on the last product they presumably produce (from zeta-carotene to didehydrolycopene). Carotene diversity can be further expanded in later steps with the addition of one or two rings by lycopene cyclases, thereby producing an extensive variety of symmetrical or asymmetrical cyclised carotenes, such as beta-zeacarotene, dehydro-beta-carotene, gamma-carotene, beta-carotene, and the fungi-specific torulene. When expressed in heterologous hosts, CrtI enzymes exhibit distinct desaturation patterns, CrtI enzyme activities may depend on the experimental conditions and thus be inconsistent with the patterns generated in the natural host. Enterobacter agglomerans CrtI produces lycopene in vivo and in vitro, but also tetradehydrolycopene in vitro | Pantoea agglomerans |
additional information | comparison of the natural evolution and kinetic properties of selected CrtI enzymes expressed and assayed under standardised conditions. Potentially all CrtI enzymes can catalyse desaturation reactions that progress beyond the already observed end-products and the pattern of products formed originates from variations in the reaction rates rather than affinity constants | Pantoea ananatis |
additional information | comparison of the natural evolution and kinetic properties of selected CrtI enzymes expressed and assayed under standardised conditions. Potentially all CrtI enzymes can catalyse desaturation reactions that progress beyond the already observed end-products and the pattern of products formed originates from variations in the reaction rates rather than affinity constants | Blakeslea trispora |
additional information | comparison of the natural evolution and kinetic properties of selected CrtI enzymes expressed and assayed under standardised conditions. Potentially all CrtI enzymes can catalyse desaturation reactions that progress beyond the already observed end-products and the pattern of products formed originates from variations in the reaction rates rather than affinity constants | Mycolicibacterium aurum |
additional information | comparison of the natural evolution and kinetic properties of selected CrtI enzymes expressed and assayed under standardised conditions. Potentially all CrtI enzymes can catalyse desaturation reactions that progress beyond the already observed end-products and the pattern of products formed originates from variations in the reaction rates rather than affinity constants | Rhodospirillum rubrum |
additional information | comparison of the natural evolution and kinetic properties of selected CrtI enzymes expressed and assayed under standardised conditions. Potentially all CrtI enzymes can catalyse desaturation reactions that progress beyond the already observed end-products and the pattern of products formed originates from variations in the reaction rates rather than affinity constants | Pantoea agglomerans |